Restriction map for
pF1KSDA1383
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI Alw44I AlwI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbrPI BbvCI BclI BfrI
BglII BlnI BsaAI BsaBI BsiWI
BsmBI BsoBI BspDI BssHII BssSI
Bst1107I BstBI BstYI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I Eco47III
EcoRV EcoT14I FbaI FspI HindIII
HpaI KpnI KspI MamI MfeI
MflI MluI MunI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccIII 454 TCCGGA T^CCGGA
AocI 988 CCTGAGG CC^T[ACGT]AGG
AseI 1165 ATTAAT AT^TAAT
AsnI 1165 ATTAAT AT^TAAT
AspHI 2474 GAGCTC G[AT]GC[AT]^C
AvaII 329 GGACC G^G[AT]CC
BalI 416 TGGCCA TGG^CCA
BbeI 368 GGCGCC GGCGC^C
BbsI 1385 GTCTTC GAAGAC(2/6)
BcgI 1305 CGAATCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2704 GGATAC GTATCC(6/5)
BglI 360 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 564 GCTGAGC GC^T[ACGT]AGC
BpmI 34 CTGGAG CTGGAG(16/14)
Bpu1102I 564 GCTGAGC GC^T[ACGT]AGC
BpuAI 1385 GTCTTC GAAGAC(2/6)
BsaI 883 GAGACC GGTCTC(1/5)
BsaWI 454 TCCGGA [AT]^CCGG[AT]
BseAI 454 TCCGGA T^CCGGA
BsiHKAI 2474 GAGCTC G[AT]GC[AT]^C
BsmAI 883 GAGAC GTCTC(1/5)
BsmI 981 GAATGC GAATGC(1/-1)
Bsp1407I 2313 TGTACA T^GTACA
BspEI 454 TCCGGA T^CCGGA
BspHI 2175 TCATGA T^CATGA
BspMI 2561 ACCTGC ACCTGC(4/8)
BsrGI 2313 TGTACA T^GTACA
BstAPI 275 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2306 GGTCACC G^GT[ACGT]ACC
BstPI 2306 GGTCACC G^GT[ACGT]ACC
BstXI 194 CCACGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 988 CCTGAGG CC^T[ACGT]AGG
CelII 564 GCTGAGC GC^T[ACGT]AGC
DraI 1518 TTTAAA TTT^AAA
DraII 397 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 446 CGGCCG C^GGCCG
EclXI 446 CGGCCG C^GGCCG
Eco52I 446 CGGCCG C^GGCCG
Eco81I 988 CCTGAGG CC^T[ACGT]AGG
EcoNI 852 CCTAAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 397 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 2306 GGTCACC G^GT[ACGT]ACC
EcoRI 1610 GAATTC G^AATTC
EcoT22I 1697 ATGCAT ATGCA^T
FseI 443 GGCCGGCC GGCCGG^CC
HgaI 375 GACGC GACGC(5/10)
HincII 1966 GTTGAC GT[CT]^[GA]AC
HindII 1966 GTTGAC GT[CT]^[GA]AC
KasI 368 GGCGCC G^GCGCC
MluNI 416 TGGCCA TGG^CCA
MroI 454 TCCGGA T^CCGGA
MscI 416 TGGCCA TGG^CCA
NarI 368 GGCGCC GG^CGCC
NsiI 1697 ATGCAT ATGCA^T
PshBI 1165 ATTAAT AT^TAAT
RcaI 2175 TCATGA T^CATGA
SacI 2474 GAGCTC GAGCT^C
SapI 24 GCTCTTC GCTCTTC(1/4)
SfoI 368 GGCGCC GGC^GCC
SspBI 2313 TGTACA T^GTACA
StuI 137 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 368 GGCGCC G[GA]^CG[CT]C
375 GACGCC
AflIII 814 ACATGT A^C[GA][CT]GT
1141 ACATGT
BanI 368 GGCGCC G^G[CT][GA]CC
1450 GGTGCC
BanII 1753 GAGCCC G[GA]GC[CT]^C
2474 GAGCTC
BsaHI 368 GGCGCC G[GA]^CG[CT]C
375 GACGCC
BsgI 310 CTGCAC GTGCAG(16/14)
947 CTGCAC
BsiEI 267 CGGTCG CG[GA][CT]^CG
446 CGGCCG
BspLU11I 814 ACATGT A^CATGT
1141 ACATGT
DpnII 2437 GATC ^GATC
2611 GATC
DsaI 167 CCGTGG C^C[GA][CT]GG
244 CCGTGG
EaeI 416 TGGCCA [CT]^GGCC[GA]
446 CGGCCG
HaeII 226 GGCGCT [GA]GCGC^[CT]
368 GGCGCC
Hin1I 368 GGCGCC G[GA]^CG[CT]C
375 GACGCC
MaeII 480 ACGT A^CGT
1515 ACGT
MboI 2437 GATC ^GATC
2611 GATC
MslI 2025 CATTCTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2238 CACAGGAGTG
NaeI 317 GCCGGC GCC^GGC
444 GCCGGC
NdeII 2437 GATC ^GATC
2611 GATC
NgoMIV 317 GCCGGC G^CCGGC
444 GCCGGC
PvuII 355 CAGCTG CAG^CTG
649 CAGCTG
Sau3AI 2437 GATC ^GATC
2611 GATC
TaqI 498 TCGA T^CGA
1161 TCGA
TthHB8I 498 TCGA T^CGA
1161 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI BbvI BcnI BfaI
BmyI BsaJI BseRI BsiYI BslI
BsmFI Bsp1286I BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NlaIII NlaIV NspI PleI PstI
RsaI Sau96I ScrFI SfaNI SfcI
SmlI SspI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI