Restriction map for
pF1KSDA1392
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AlwNI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbeI BbvCI BclI BfrI BlnI
BlpI Bpu1102I BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI DraIII EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI SacI SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 470 GGTACC G^GTACC
AgeI 497 ACCGGT A^CCGGT
AocI 545 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1702 AGCGCT AGC^GCT
ApaI 1989 GGGCCC GGGCC^C
Asp718I 470 GGTACC G^GTACC
BbrPI 306 CACGTG CAC^GTG
BciVI 488 GGATAC GTATCC(6/5)
BglI 1947 GCCTGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 383 AGATCT A^GATCT
BsaBI 933 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 998 TCATGA T^CATGA
BsrBI 2419 GAGCGG CCGCTC(-3/-3)
BsrFI 497 ACCGGT [GA]^CCGG[CT]
Bsu36I 545 CCTCAGG CC^T[ACGT]AGG
Cfr10I 497 ACCGGT [GA]^CCGG[CT]
DraI 823 TTTAAA TTT^AAA
DrdI 1125 GACACGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1702 AGCGCT AGC^GCT
Eco81I 545 CCTCAGG CC^T[ACGT]AGG
EcoRV 1168 GATATC GAT^ATC
HaeII 1702 AGCGCT [GA]GCGC^[CT]
HindIII 2759 AAGCTT A^AGCTT
KpnI 470 GGTACC GGTAC^C
KspI 122 CCGCGG CCGC^GG
MamI 933 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 627 GCATGC [GA]CATG^[CT]
PinAI 497 ACCGGT A^CCGGT
PmaCI 306 CACGTG CAC^GTG
PmlI 306 CACGTG CAC^GTG
RcaI 998 TCATGA T^CATGA
SacII 122 CCGCGG CCGC^GG
SmaI 523 CCCGGG CCC^GGG
SphI 627 GCATGC GCATG^C
StuI 1600 AGGCCT AGG^CCT
TfiI 2570 GATTC G^A[AT]TC
XmaCI 523 CCCGGG C^CCGGG
XmaI 523 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 1521 GACGCC G[GA]^CG[CT]C
2033 GACGCC
BalI 239 TGGCCA TGG^CCA
848 TGGCCA
BcgI 577 CGAAACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
250 GCAATGAACTCG
BpmI 395 CTCCAG CTGGAG(16/14)
1938 CTCCAG
BsaAI 306 CACGTG [CT]AC^GT[GA]
2582 CACGTA
BsaHI 1521 GACGCC G[GA]^CG[CT]C
2033 GACGCC
BsgI 106 CTGCAC GTGCAG(16/14)
1604 CTGCAC
BspMI 2190 ACCTGC ACCTGC(4/8)
1923 GCAGGT
BstF5I 1748 CATCC GGATG(2/0)
1320 GGATG
CpoI 56 CGGACCG CG^G[AT]CCG
68 CGGACCG
EaeI 239 TGGCCA [CT]^GGCC[GA]
848 TGGCCA
EarI 2117 CTCTTC CTCTTC(1/4)
877 GAAGAG
FokI 1748 CATCC GGATG(9/13)
1320 GGATG
Hin1I 1521 GACGCC G[GA]^CG[CT]C
2033 GACGCC
Ksp632I 2117 CTCTTC CTCTTC(1/4)
877 GAAGAG
MluNI 239 TGGCCA TGG^CCA
848 TGGCCA
MscI 239 TGGCCA TGG^CCA
848 TGGCCA
PaeR7I 1288 CTCGAG C^TCGAG
2416 CTCGAG
PflMI 1747 CCATCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2402 CCATGCAGTGG
PstI 603 CTGCAG CTGCA^G
1545 CTGCAG
PvuII 844 CAGCTG CAG^CTG
1175 CAGCTG
RsrII 56 CGGACCG CG^G[AT]CCG
68 CGGACCG
ScaI 724 AGTACT AGT^ACT
1591 AGTACT
SfaNI 1356 GATGC GCATC(5/9)
2552 GCATC
SmlI 1288 CTCGAG C^T[CT][GA]AG
2416 CTCGAG
Van91I 1747 CCATCCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2402 CCATGCAGTGG
XhoI 1288 CTCGAG C^TCGAG
2416 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AcsI AfaI AhdI
AluI Alw44I AlwI ApaLI ApoI
AspEI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpuAI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI Eam1105I EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII