Restriction map for
pF1KSDA1397
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI Aor51HI ApaI
AscI Asp700I Asp718I AspEI AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraII Eam1105I Eco47III Eco81I
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NarI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PpuMI
Psp1406I PvuI RcaI RsrII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1180 GACGTC GACGT^C
AcyI 1180 GACGTC G[GA]^CG[CT]C
AflIII 511 ACGTGT A^C[GA][CT]GT
Alw44I 876 GTGCAC G^TGCAC
ApaLI 876 GTGCAC G^TGCAC
AseI 1957 ATTAAT AT^TAAT
AsnI 1957 ATTAAT AT^TAAT
AviII 1709 TGCGCA TGC^GCA
BsaHI 1180 GACGTC G[GA]^CG[CT]C
BseRI 155 GAGGAG GAGGAG(10/8)
BsmBI 111 GAGACG CGTCTC(1/5)
BsmI 596 GAATGC GAATGC(1/-1)
BsrFI 1303 GCCGGC [GA]^CCGG[CT]
BstEII 1051 GGTCACC G^GT[ACGT]ACC
BstPI 1051 GGTCACC G^GT[ACGT]ACC
BstYI 1263 AGATCC [GA]^GATC[CT]
Cfr10I 1303 GCCGGC [GA]^CCGG[CT]
DraIII 1114 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1174 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 557 CGGCCG C^GGCCG
EarI 957 GAAGAG CTCTTC(1/4)
EclXI 557 CGGCCG C^GGCCG
Eco52I 557 CGGCCG C^GGCCG
EcoNI 666 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1051 GGTCACC G^GT[ACGT]ACC
FspI 1709 TGCGCA TGC^GCA
HaeII 1025 AGCGCC [GA]GCGC^[CT]
HgaI 113 GACGC GACGC(5/10)
Hin1I 1180 GACGTC G[GA]^CG[CT]C
HincII 361 GTCAAC GT[CT]^[GA]AC
HindII 361 GTCAAC GT[CT]^[GA]AC
Ksp632I 957 GAAGAG CTCTTC(1/4)
MflI 1263 AGATCC [GA]^GATC[CT]
NaeI 1303 GCCGGC GCC^GGC
NdeI 519 CATATG CA^TATG
NgoMIV 1303 GCCGGC G^CCGGC
NheI 1606 GCTAGC G^CTAGC
NspI 641 GCATGT [GA]CATG^[CT]
PshAI 473 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1957 ATTAAT AT^TAAT
PstI 1129 CTGCAG CTGCA^G
SacI 1681 GAGCTC GAGCT^C
ScaI 205 AGTACT AGT^ACT
SexAI 2010 ACCAGGT A^CC[AT]GGT
SfcI 1129 CTGCAG C^T[GA][CT]AG
SmaI 1667 CCCGGG CCC^GGG
SmlI 413 CTCAAG C^T[CT][GA]AG
XhoII 1263 AGATCC [GA]^GATC[CT]
XmaCI 1667 CCCGGG C^CCGGG
XmaI 1667 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 1264 GATCC GGATC(4/5)
1631 GGATC
AspI 628 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1282 GACTTTGTC
AvaI 789 CTCGGG C^[CT]CG[GA]G
1667 CCCGGG
BalI 137 TGGCCA TGG^CCA
290 TGGCCA
BbrPI 294 CACGTG CAC^GTG
393 CACGTG
BbsI 1762 GAAGAC GAAGAC(2/6)
1969 GTCTTC
BfaI 182 CTAG C^TAG
1607 CTAG
BpuAI 1762 GAAGAC GAAGAC(2/6)
1969 GTCTTC
BsiEI 366 CGACCG CG[GA][CT]^CG
557 CGGCCG
BsoBI 789 CTCGGG C^[CT]CG[GA]G
1667 CCCGGG
BsrBI 843 CCGCTC CCGCTC(-3/-3)
1312 GAGCGG
BssSI 619 CACGAG CACGAG(-5/-1)
1018 CTCGTG
MaeI 182 CTAG C^TAG
1607 CTAG
MluNI 137 TGGCCA TGG^CCA
290 TGGCCA
MscI 137 TGGCCA TGG^CCA
290 TGGCCA
NcoI 965 CCATGG C^CATGG
1946 CCATGG
PflFI 628 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1282 GACTTTGTC
PflMI 282 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCATCTGCTGG
PmaCI 294 CACGTG CAC^GTG
393 CACGTG
PmlI 294 CACGTG CAC^GTG
393 CACGTG
PvuII 1132 CAGCTG CAG^CTG
1527 CAGCTG
TfiI 327 GATTC G^A[AT]TC
1060 GATTC
Tth111I 628 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1282 GACTTTGTC
Van91I 282 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1040 CCATCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaII BanII
BbvI BcnI BmyI BpmI BsaAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI KspI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I