##################################
# NO CUT ENZYMES (   40 enzymes) #
##################################

AccIII      AclI        AflII       AgeI        AscI        
AseI        AsnI        AvrII       BbeI        BfrI        
BlnI        BseAI       BsiWI       BspEI       BstBI       
CpoI        DraI        FseI        KasI        MfeI        
MluI        MroI        MunI        NarI        NdeI        
NheI        NruI        NspV        PinAI       PmeI        
PshAI       PshBI       Psp1406I    RsrII       SfoI        
SfuI        SgrAI       SnaBI       SspI        SwaI        


###################################
# ONE CUT ENZYMES (   26 enzymes) #
###################################

Acc65I       6570  GGTACC               G^GTACC
Asp718I      6570  GGTACC               G^GTACC
BbrPI        2909  CACGTG               CAC^GTG
BciVI        8628  GGATAC               GTATCC(6/5)
BssHII       9120  GCGCGC               G^CGCGC
Bst1107I     8007  GTATAC               GTA^TAC
BstZ17I      8007  GTATAC               GTA^TAC
EcoRV        7966  GATATC               GAT^ATC
EcoT22I      9291  ATGCAT               ATGCA^T
HindIII       411  AAGCTT               A^AGCTT
HpaI        12465  GTTAAC               GTT^AAC
KpnI         6570  GGTACC               GGTAC^C
KspI         7063  CCGCGG               CCGC^GG
NotI         7769  GCGGCCGC             GC^GGCCGC
NsiI         9291  ATGCAT               ATGCA^T
PacI         1936  TTAATTAA             TTAAT^TAA
PaeR7I       1590  CTCGAG               C^TCGAG
PmaCI        2909  CACGTG               CAC^GTG
PmlI         2909  CACGTG               CAC^GTG
PvuI         9610  CGATCG               CGAT^CG
SacII        7063  CCGCGG               CCGC^GG
SalI         3507  GTCGAC               G^TCGAC
SfiI         4245  GGCCATCAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI        12069  ACTAGT               A^CTAGT
XbaI         1290  TCTAGA               T^CTAGA
XhoI         1590  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AatII        5637  GACGTC               GACGT^C
            11968  GACGTC              
AccI         3507  GTCGAC               GT^[AC][GT]AC
             8007  GTATAC              
AocI           43  CCTCAGG              CC^T[ACGT]AGG
             7317  CCTGAGG             
BsaWI        4702  ACCGGA               [AT]^CCGG[AT]
            10686  ACCGGA              
BsmBI        9403  GAGACG               CGTCTC(1/5)
            11881  GAGACG              
BspLU11I     4118  ACATGT               A^CATGT
             5834  ACATGT              
Bsu36I         43  CCTCAGG              CC^T[ACGT]AGG
             7317  CCTGAGG             
EagI         4040  CGGCCG               C^GGCCG
             7770  CGGCCG              
EclXI        4040  CGGCCG               C^GGCCG
             7770  CGGCCG              
Eco52I       4040  CGGCCG               C^GGCCG
             7770  CGGCCG              
Eco81I         43  CCTCAGG              CC^T[ACGT]AGG
             7317  CCTGAGG             


#########################################
# 3 OR MORE CUT ENZYMES (  181 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AflIII      AhdI        AluI        Alw44I      AlwI        
AlwNI       Aor51HI     ApaI        ApaLI       ApoI        
Asp700I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       BalI        BamHI       BanI        
BanII       BbsI        BbvCI       BbvI        BcgI        
BclI        BcnI        BfaI        BglI        BglII       
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BsaJI       
BseRI       BsgI        BsiEI       BsiHKAI     BsiYI       
BslI        BsmAI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspHI       BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssKI       BssSI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
ClaI        DdeI        DpnII       DraII       DraIII      
DrdI        DsaI        EaeI        Eam1105I    EarI        
Eco47III    EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoT14I     FauI        FbaI        Fnu4HI      
FokI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MamI        
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NaeI        NciI        
NcoI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflFI       PflMI       PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfaNI       SfcI        SmaI        
SmlI        SphI        Sse8387I    SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
Van91I      XcmI        XhoII       XmaCI       XmaI        
XmnI