################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI AlwI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanII BbeI BbsI BbvCI BcgI BciVI BfrI BlnI BpuAI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspMI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DrdI Eam1105I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HincII HindII HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### AcsI 280 GAATTT [GA]^AATT[CT] AcyI 73 GACGCC G[GA]^CG[CT]C AfaI 392 GTAC GT^AC Alw44I 423 GTGCAC G^TGCAC AlwNI 736 CAGTCGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 983 AGCGCT AGC^GCT ApaLI 423 GTGCAC G^TGCAC ApoI 280 GAATTT [GA]^AATT[CT] AspHI 423 GTGCAC G[AT]GC[AT]^C AspI 73 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 445 CTCGGG C^[CT]CG[GA]G BalI 371 TGGCCA TGG^CCA BanI 65 GGCACC G^G[CT][GA]CC BbrPI 561 CACGTG CAC^GTG BfaI 1075 CTAG C^TAG BglI 76 GCCGTCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1172 AGATCT A^GATCT BlpI 48 GCTGAGC GC^T[ACGT]AGC BpmI 18 CTCCAG CTGGAG(16/14) Bpu1102I 48 GCTGAGC GC^T[ACGT]AGC BsaAI 561 CACGTG [CT]AC^GT[GA] BsaBI 1057 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 73 GACGCC G[GA]^CG[CT]C BseRI 833 CTCCTC GAGGAG(10/8) BsiHKAI 423 GTGCAC G[AT]GC[AT]^C BsmBI 771 CGTCTC CGTCTC(1/5) BsoBI 445 CTCGGG C^[CT]CG[GA]G BspLU11I 1112 ACATGT A^CATGT BsrBI 432 GAGCGG CCGCTC(-3/-3) BsrDI 1125 CATTGC GCAATG(2/0) BsrFI 394 ACCGGC [GA]^CCGG[CT] BstAPI 941 GCAGGAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1143 GGTGACC G^GT[ACGT]ACC BstPI 1143 GGTGACC G^GT[ACGT]ACC BstYI 1172 AGATCT [GA]^GATC[CT] CelII 48 GCTGAGC GC^T[ACGT]AGC Cfr10I 394 ACCGGC [GA]^CCGG[CT] DraII 620 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 375 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1118 CCATGG C^C[GA][CT]GG Eco47III 983 AGCGCT AGC^GCT EcoO109I 620 GGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 1143 GGTGACC G^GT[ACGT]ACC HaeII 983 AGCGCT [GA]GCGC^[CT] Hin1I 73 GACGCC G[GA]^CG[CT]C HindIII 547 AAGCTT A^AGCTT MaeI 1075 CTAG C^TAG MamI 1057 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 1172 AGATCT [GA]^GATC[CT] MluNI 371 TGGCCA TGG^CCA MscI 371 TGGCCA TGG^CCA MseI 302 TTAA T^TAA MspA1I 631 CAGCGG C[AC]G^C[GT]G NcoI 1118 CCATGG C^CATGG PflFI 73 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1045 GACTC GAGTC(4/5) PmaCI 561 CACGTG CAC^GTG PmlI 561 CACGTG CAC^GTG PpuMI 620 GGGACCC [GA]G^G[AT]CC[CT] PshAI 163 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsaI 392 GTAC GT^AC SmlI 987 CTCAAG C^T[CT][GA]AG Tru9I 302 TTAA T^TAA Tth111I 73 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1130 CCAGGGGACTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1172 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 87 CGCG CG^CG 428 CGCG AvaII 621 GGACC G^G[AT]CC 1154 GGACC BclI 116 TGATCA T^GATCA 386 TGATCA BmyI 64 GGGCAC G[AGT]GC[ACT]^C 423 GTGCAC BsgI 733 GTGCAG GTGCAG(16/14) 972 GTGCAG BsmAI 161 GAGAC GTCTC(1/5) 772 GTCTC Bsp1286I 64 GGGCAC G[AGT]GC[ACT]^C 423 GTGCAC BstUI 87 CGCG CG^CG 428 CGCG CfoI 88 GCGC GCG^C 984 GCGC EagI 5 CGGCCG C^GGCCG 83 CGGCCG EarI 640 CTCTTC CTCTTC(1/4) 826 GAAGAG EclXI 5 CGGCCG C^GGCCG 83 CGGCCG Eco52I 5 CGGCCG C^GGCCG 83 CGGCCG EcoT14I 101 CCAAGG C^C[AT][AT]GG 1118 CCATGG FbaI 116 TGATCA T^GATCA 386 TGATCA HhaI 88 GCGC GCG^C 984 GCGC HinP1I 88 GCGC G^CGC 984 GCGC Ksp632I 640 CTCTTC CTCTTC(1/4) 826 GAAGAG MslI 666 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1113 CATGTCCATG MvnI 87 CGCG CG^CG 428 CGCG NspI 440 ACATGC [GA]CATG^[CT] 1112 ACATGT PflMI 192 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 374 CCACAGAGTGG PstI 939 CTGCAG CTGCA^G 1200 CTGCAG SfaNI 652 GCATC GCATC(5/9) 665 GCATC SfcI 939 CTGCAG C^T[GA][CT]AG 1200 CTGCAG StyI 101 CCAAGG C^C[AT][AT]GG 1118 CCATGG TaqI 59 TCGA T^CGA 1010 TCGA TfiI 853 GATTC G^A[AT]TC 1033 GATTC Tsp45I 79 GTCAC ^GT[GC]AC 1144 GTGAC Tsp509I 281 AATT ^AATT 795 AATT TthHB8I 59 TCGA T^CGA 1010 TCGA Van91I 192 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 374 CCACAGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 50 enzymes) # ######################################### AciI AflIII AluI BbvI BcnI BsaJI BsiEI BsiYI BslI BsmFI BspHI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV RcaI Sau3AI Sau96I ScrFI TseI TspRI