Restriction map for
pF1KSDA1437
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
Acc65I AclI AflIII AgeI AocI
AscI AseI AsnI Asp718I AviII
AvrII BbeI BbrPI BciVI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssHII BstBI BstXI
Bsu36I CelII ClaI CpoI DraI
DrdI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 1099 GACGTC GACGT^C
AcsI 433 AAATTC [GA]^AATT[CT]
AflII 1930 CTTAAG C^TTAAG
AhdI 583 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1163 AGCGCT AGC^GCT
ApoI 433 AAATTC [GA]^AATT[CT]
AspEI 583 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 642 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2386 CCCGAG C^[CT]CG[GA]G
BalI 941 TGGCCA TGG^CCA
BamHI 53 GGATCC G^GATCC
BbvCI 2073 CCTCAGC CCTCAGC(-5/-2)
BcgI 2012 GCAACAAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 815 TGATCA T^GATCA
BfrI 1930 CTTAAG C^TTAAG
BsiEI 596 CGACCG CG[GA][CT]^CG
BsoBI 2386 CCCGAG C^[CT]CG[GA]G
BssSI 507 CACGAG CACGAG(-5/-1)
Bst1107I 1011 GTATAC GTA^TAC
BstAPI 2229 GCAGTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1011 GTATAC GTA^TAC
DraIII 463 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 583 GACAAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1163 AGCGCT AGC^GCT
EcoNI 1179 CCTGTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 815 TGATCA T^GATCA
MluNI 941 TGGCCA TGG^CCA
MscI 941 TGGCCA TGG^CCA
MseI 1931 TTAA T^TAA
NaeI 552 GCCGGC GCC^GGC
NcoI 578 CCATGG C^CATGG
NgoMIV 552 GCCGGC G^CCGGC
PflFI 642 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 933 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1210 CAGCTG CAG^CTG
SbfI 1845 CCTGCAGG CCTGCA^GG
Sse8387I 1845 CCTGCAGG CCTGCA^GG
Tru9I 1931 TTAA T^TAA
Tsp509I 434 AATT ^AATT
Tth111I 642 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 933 CCACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 446 CCAGCTCGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 847 GTCTAC GT^[AC][GT]AC
1011 GTATAC
AccIII 749 TCCGGA T^CCGGA
2172 TCCGGA
AcyI 963 GGCGTC G[GA]^CG[CT]C
1099 GACGTC
Alw44I 856 GTGCAC G^TGCAC
1699 GTGCAC
ApaLI 856 GTGCAC G^TGCAC
1699 GTGCAC
BanI 308 GGCACC G^G[CT][GA]CC
1732 GGCACC
BcnI 207 CCGGG CC^[GC]GG
228 CCCGG
BfaI 982 CTAG C^TAG
2137 CTAG
BsaAI 322 TACGTG [CT]AC^GT[GA]
853 TACGTG
BsaHI 963 GGCGTC G[GA]^CG[CT]C
1099 GACGTC
BseAI 749 TCCGGA T^CCGGA
2172 TCCGGA
BsmBI 682 GAGACG CGTCTC(1/5)
2161 GAGACG
BsmFI 127 GGGAC GGGAC(10/14)
770 GGGAC
BspEI 749 TCCGGA T^CCGGA
2172 TCCGGA
BsrDI 1392 CATTGC GCAATG(2/0)
194 GCAATG
BstEII 138 GGTCACC G^GT[ACGT]ACC
177 GGTCACC
BstPI 138 GGTCACC G^GT[ACGT]ACC
177 GGTCACC
EcoO65I 138 GGTCACC G^GT[ACGT]ACC
177 GGTCACC
Hin1I 963 GGCGTC G[GA]^CG[CT]C
1099 GACGTC
MaeI 982 CTAG C^TAG
2137 CTAG
MroI 749 TCCGGA T^CCGGA
2172 TCCGGA
NciI 207 CCGGG CC^[GC]GG
228 CCCGG
NspI 791 ACATGC [GA]CATG^[CT]
1007 GCATGT
PpuMI 1346 AGGTCCT [GA]G^G[AT]CC[CT]
2363 AGGACCT
SapI 2040 GCTCTTC GCTCTTC(1/4)
2178 GCTCTTC
ScaI 368 AGTACT AGT^ACT
1049 AGTACT
SexAI 380 ACCTGGT A^CC[AT]GGT
1331 ACCTGGT
TfiI 3 GATTC G^A[AT]TC
199 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI Asp700I AspHI AvaII
BanII BbsI BbvI BglI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI PstI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TseI
Tsp45I TspRI TthHB8I XhoII XmnI