Restriction map for
pF1KSDA1439
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AvaI AviII AvrII BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I CelII ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoT22I FbaI
FseI FspI Hin1I HpaI KasI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspBI SspI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 1517 GGTACC G^GTACC
Alw44I 1279 GTGCAC G^TGCAC
AlwNI 713 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1174 CCTCAGG CC^T[ACGT]AGG
ApaLI 1279 GTGCAC G^TGCAC
Asp700I 49 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1517 GGTACC G^GTACC
AspI 1506 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1334 TGGCCA TGG^CCA
BamHI 1476 GGATCC G^GATCC
BcgI 1087 GCAACACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 682 CTAG C^TAG
BpmI 378 CTGGAG CTGGAG(16/14)
BsgI 1397 GTGCAG GTGCAG(16/14)
BsmBI 1180 GAGACG CGTCTC(1/5)
BsmI 947 GAATGC GAATGC(1/-1)
BspMI 95 ACCTGC ACCTGC(4/8)
BsrBI 10 CCGCTC CCGCTC(-3/-3)
BsrDI 781 GCAATG GCAATG(2/0)
BsrFI 1353 GCCGGT [GA]^CCGG[CT]
BsrI 718 ACTGG ACTGG(1/-1)
BstXI 1128 CCAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1476 GGATCC [GA]^GATC[CT]
Bsu36I 1174 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1353 GCCGGT [GA]^CCGG[CT]
DpnII 1477 GATC ^GATC
DraI 409 TTTAAA TTT^AAA
DraII 1065 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 643 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1471 CCACGG C^C[GA][CT]GG
EaeI 1334 TGGCCA [CT]^GGCC[GA]
Eco81I 1174 CCTCAGG CC^T[ACGT]AGG
EcoNI 357 CCTCCGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1065 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 244 GATATC GAT^ATC
FauI 1444 CCCGC CCCGC(4/6)
HapII 1354 CCGG C^CGG
HincII 1383 GTCAAC GT[CT]^[GA]AC
HindII 1383 GTCAAC GT[CT]^[GA]AC
HindIII 1482 AAGCTT A^AGCTT
HpaII 1354 CCGG C^CGG
KpnI 1517 GGTACC GGTAC^C
MaeI 682 CTAG C^TAG
MboI 1477 GATC ^GATC
MflI 1476 GGATCC [GA]^GATC[CT]
MluNI 1334 TGGCCA TGG^CCA
MscI 1334 TGGCCA TGG^CCA
MslI 1299 CACCCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 421 CCGCTG C[AC]G^C[GT]G
MspI 1354 CCGG C^CGG
NdeII 1477 GATC ^GATC
PflFI 1506 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1065 AGGACCC [GA]G^G[AT]CC[CT]
SacI 816 GAGCTC GAGCT^C
Sau3AI 1477 GATC ^GATC
SbfI 96 CCTGCAGG CCTGCA^GG
ScaI 430 AGTACT AGT^ACT
SmlI 832 CTCAAG C^T[CT][GA]AG
Sse8387I 96 CCTGCAGG CCTGCA^GG
StuI 1339 AGGCCT AGG^CCT
Tth111I 1506 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 612 CCAGCCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1476 GGATCC [GA]^GATC[CT]
XmnI 49 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 431 GTAC GT^AC
1518 GTAC
AlwI 1477 GATCC GGATC(4/5)
1476 GGATC
AspHI 816 GAGCTC G[AT]GC[AT]^C
1279 GTGCAC
BanI 1356 GGTGCC G^G[CT][GA]CC
1517 GGTACC
BanII 478 GGGCTC G[GA]GC[CT]^C
816 GAGCTC
BsiHKAI 816 GAGCTC G[AT]GC[AT]^C
1279 GTGCAC
BsmAI 1180 GAGAC GTCTC(1/5)
17 GTCTC
BsmFI 456 GTCCC GGGAC(10/14)
578 GTCCC
EarI 1100 CTCTTC CTCTTC(1/4)
154 GAAGAG
EcoT14I 888 CCAAGG C^C[AT][AT]GG
1269 CCAAGG
HgaI 595 GACGC GACGC(5/10)
1182 GACGC
Ksp632I 1100 CTCTTC CTCTTC(1/4)
154 GAAGAG
NspI 549 GCATGC [GA]CATG^[CT]
1083 GCATGC
PflMI 616 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
901 CCAGGAAGTGG
PleI 1098 GACTC GAGTC(4/5)
771 GAGTC
PstI 97 CTGCAG CTGCA^G
710 CTGCAG
RsaI 431 GTAC GT^AC
1518 GTAC
SphI 549 GCATGC GCATG^C
1083 GCATGC
StyI 888 CCAAGG C^C[AT][AT]GG
1269 CCAAGG
TfiI 559 GATTC G^A[AT]TC
1454 GATTC
Van91I 616 CCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
901 CCAGGAAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeII HaeIII HhaI
HinP1I HinfI HphI ItaI MaeII
MaeIII MboII MnlI MseI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I