Restriction map for
pF1KSDA1442
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AseI
AsnI Asp700I Asp718I AviII AvrII
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BseAI
BsiWI BspDI BspEI BspHI BsrDI
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI Eco47III
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
MaeI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tsp509I XbaI
XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 997 GTCTAC GT^[AC][GT]AC
AhdI 385 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 691 GTGCAC G^TGCAC
AlwNI 1159 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 174 CCTCAGG CC^T[ACGT]AGG
ApaI 1327 GGGCCC GGGCC^C
ApaLI 691 GTGCAC G^TGCAC
AspEI 385 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 526 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 311 GGATCC G^GATCC
BanI 799 GGCGCC G^G[CT][GA]CC
BbeI 799 GGCGCC GGCGC^C
BbrPI 661 CACGTG CAC^GTG
BsaI 1073 GAGACC GGTCTC(1/5)
BseRI 37 GAGGAG GAGGAG(10/8)
BsmI 591 GAATGC GAATGC(1/-1)
Bsp1407I 1202 TGTACA T^GTACA
BspLU11I 683 ACATGT A^CATGT
BspMI 843 GCAGGT ACCTGC(4/8)
BsrGI 1202 TGTACA T^GTACA
BsrI 1612 CCAGT ACTGG(1/-1)
BstEII 942 GGTGACC G^GT[ACGT]ACC
BstPI 942 GGTGACC G^GT[ACGT]ACC
BstYI 311 GGATCC [GA]^GATC[CT]
Bsu36I 174 CCTCAGG CC^T[ACGT]AGG
DraIII 196 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 385 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 492 GAAGAG CTCTTC(1/4)
Eco81I 174 CCTCAGG CC^T[ACGT]AGG
EcoNI 30 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 942 GGTGACC G^GT[ACGT]ACC
FseI 1461 GGCCGGCC GGCCGG^CC
KasI 799 GGCGCC G^GCGCC
Ksp632I 492 GAAGAG CTCTTC(1/4)
MflI 311 GGATCC [GA]^GATC[CT]
NarI 799 GGCGCC GG^CGCC
PflFI 526 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 624 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 385 GACTC GAGTC(4/5)
PmaCI 661 CACGTG CAC^GTG
PmlI 661 CACGTG CAC^GTG
PpuMI 1480 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 1353 CTGCAG CTGCA^G
PvuII 1350 CAGCTG CAG^CTG
SacI 877 GAGCTC GAGCT^C
SapI 492 GAAGAGC GCTCTTC(1/4)
SfoI 799 GGCGCC GGC^GCC
SmlI 553 CTCAAG C^T[CT][GA]AG
SspBI 1202 TGTACA T^GTACA
StuI 1575 AGGCCT AGG^CCT
TspRI 68 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 526 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 624 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 311 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 660 ACACGT A^C[GA][CT]GT
683 ACATGT
AlwI 312 GATCC GGATC(4/5)
311 GGATC
AscI 720 GGCGCGCC GG^CGCGCC
1447 GGCGCGCC
BalI 206 TGGCCA TGG^CCA
1298 TGGCCA
BsaAI 370 TACGTG [CT]AC^GT[GA]
661 CACGTG
BsaWI 488 ACCGGA [AT]^CCGG[AT]
506 ACCGGA
BsgI 471 GTGCAG GTGCAG(16/14)
904 GTGCAG
BsmBI 376 CGTCTC CGTCTC(1/5)
514 GAGACG
BsrBI 1228 CCGCTC CCGCTC(-3/-3)
1404 GAGCGG
BssSI 140 CACGAG CACGAG(-5/-1)
865 CTCGTG
BstAPI 473 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
974 GCAAGGGATGC
DpnII 312 GATC ^GATC
465 GATC
DraII 1326 GGGGCCC [GA]G^G[ACGT]CC[CT]
1480 GGGTCCC
EagI 987 CGGCCG C^GGCCG
1115 CGGCCG
EclXI 987 CGGCCG C^GGCCG
1115 CGGCCG
Eco52I 987 CGGCCG C^GGCCG
1115 CGGCCG
EcoO109I 1326 GGGGCCC [GA]G^G[ACGT]CC[CT]
1480 GGGTCCC
EcoT14I 1091 CCAAGG C^C[AT][AT]GG
1526 CCAAGG
HaeII 799 GGCGCC [GA]GCGC^[CT]
1532 AGCGCC
MboI 312 GATC ^GATC
465 GATC
MluNI 206 TGGCCA TGG^CCA
1298 TGGCCA
MscI 206 TGGCCA TGG^CCA
1298 TGGCCA
NaeI 55 GCCGGC GCC^GGC
1462 GCCGGC
NdeII 312 GATC ^GATC
465 GATC
NgoMIV 55 GCCGGC G^CCGGC
1462 GCCGGC
NspI 611 ACATGC [GA]CATG^[CT]
683 ACATGT
Sau3AI 312 GATC ^GATC
465 GATC
SfcI 1045 CTACAG C^T[GA][CT]AG
1353 CTGCAG
StyI 1091 CCAAGG C^C[AT][AT]GG
1526 CCAAGG
TfiI 600 GAATC G^A[AT]TC
1034 GATTC
Tsp45I 485 GTGAC ^GT[GC]AC
943 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanII BbvI
BcnI BglI BmyI BsaHI BsaJI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BssHII
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DsaI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KspI MaeII MaeIII MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV RsaI
SacII Sau96I ScrFI SfaNI SmaI
TaqI TseI TthHB8I XmaCI XmaI