Restriction map for
pF1KSDA1453
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccI AclI AflII AgeI ApaI
AscI AseI AsnI Asp700I AvrII
BamHI BbeI BbrPI BciVI BclI
BfrI BlnI BsiWI BspDI BspHI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DrdI EcoO65I EcoRV EcoT22I FbaI
FseI HpaI KasI KspI MfeI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 1181 GGTACC G^GTACC
AccIII 1035 TCCGGA T^CCGGA
AhdI 2368 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1114 GTGCAC G^TGCAC
AocI 2353 CCTCAGG CC^T[ACGT]AGG
ApaLI 1114 GTGCAC G^TGCAC
Asp718I 1181 GGTACC G^GTACC
AspEI 2368 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 283 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2974 TGCGCA TGC^GCA
BanI 1181 GGTACC G^G[CT][GA]CC
BbvCI 3348 CCTCAGC CCTCAGC(-5/-2)
BglII 1687 AGATCT A^GATCT
BlpI 3018 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3018 GCTGAGC GC^T[ACGT]AGC
BsaWI 1035 TCCGGA [AT]^CCGG[AT]
BseAI 1035 TCCGGA T^CCGGA
BsgI 2785 CTGCAC GTGCAG(16/14)
Bsp1407I 2618 TGTACA T^GTACA
BspEI 1035 TCCGGA T^CCGGA
BspLU11I 899 ACATGT A^CATGT
BsrGI 2618 TGTACA T^GTACA
Bsu36I 2353 CCTCAGG CC^T[ACGT]AGG
CelII 3018 GCTGAGC GC^T[ACGT]AGC
DraIII 2091 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2368 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2353 CCTCAGG CC^T[ACGT]AGG
FspI 2974 TGCGCA TGC^GCA
HincII 2359 GTCAAC GT[CT]^[GA]AC
HindII 2359 GTCAAC GT[CT]^[GA]AC
KpnI 1181 GGTACC GGTAC^C
MluI 753 ACGCGT A^CGCGT
MroI 1035 TCCGGA T^CCGGA
PflFI 283 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2116 GACCTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2578 CTGCAG CTGCA^G
SbfI 2577 CCTGCAGG CCTGCA^GG
SmlI 982 CTCAAG C^T[CT][GA]AG
Sse8387I 2577 CCTGCAGG CCTGCA^GG
SspBI 2618 TGTACA T^GTACA
SspI 838 AATATT AAT^ATT
Tth111I 283 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 723 CCATCAAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AatII 808 GACGTC GACGT^C
2310 GACGTC
Aor51HI 938 AGCGCT AGC^GCT
1804 AGCGCT
BalI 1944 TGGCCA TGG^CCA
2691 TGGCCA
BbsI 3166 GAAGAC GAAGAC(2/6)
349 GTCTTC
BcgI 836 CGAATATTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2342 CGACGGCGCTGC
BpuAI 3166 GAAGAC GAAGAC(2/6)
349 GTCTTC
BsaBI 2827 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2908 GATGGGCATC
BsaI 575 GAGACC GGTCTC(1/5)
3190 GAGACC
BsmI 891 GAATGC GAATGC(1/-1)
1511 GCATTC
BsrFI 237 ACCGGC [GA]^CCGG[CT]
2107 ACCGGC
BstYI 821 AGATCC [GA]^GATC[CT]
1687 AGATCT
Cfr10I 237 ACCGGC [GA]^CCGG[CT]
2107 ACCGGC
EagI 39 CGGCCG C^GGCCG
1329 CGGCCG
EclXI 39 CGGCCG C^GGCCG
1329 CGGCCG
Eco47III 938 AGCGCT AGC^GCT
1804 AGCGCT
Eco52I 39 CGGCCG C^GGCCG
1329 CGGCCG
EcoNI 2577 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2784 CCTGCACCAGG
EcoRI 1039 GAATTC G^AATTC
1265 GAATTC
MamI 2827 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2908 GATGGGCATC
MflI 821 AGATCC [GA]^GATC[CT]
1687 AGATCT
MluNI 1944 TGGCCA TGG^CCA
2691 TGGCCA
MscI 1944 TGGCCA TGG^CCA
2691 TGGCCA
NcoI 2819 CCATGG C^CATGG
2843 CCATGG
PflMI 1393 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2694 CCAGCAGGTGG
SphI 462 GCATGC GCATG^C
494 GCATGC
Van91I 1393 CCACTGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2694 CCAGCAGGTGG
XhoII 821 AGATCC [GA]^GATC[CT]
1687 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaAI BsaHI BsaJI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I