Restriction map for
pF1KSDA1456
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI Fnu4HI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI ItaI
KasI KpnI KspI MfeI MluI
MroI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI TseI Tth111I XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 780 AAATTT [GA]^AATT[CT]
AlwI 928 GATCC GGATC(4/5)
ApaI 224 GGGCCC GGGCC^C
ApoI 780 AAATTT [GA]^AATT[CT]
Asp700I 869 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 371 GGTCC G^G[AT]CC
BalI 119 TGGCCA TGG^CCA
BcnI 136 CCCGG CC^[GC]GG
BglII 499 AGATCT A^GATCT
BpmI 495 CTCCAG CTGGAG(16/14)
BsaBI 838 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 743 GAGACC GGTCTC(1/5)
BseRI 762 GAGGAG GAGGAG(10/8)
BsmAI 743 GAGAC GTCTC(1/5)
BsmFI 372 GTCCC GGGAC(10/14)
BspMI 393 ACCTGC ACCTGC(4/8)
BstAPI 198 GCAAGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 497 CCAGATCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 823 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 966 GCGC GCG^C
DraIII 376 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 602 GACACTCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 214 CCATGG C^C[GA][CT]GG
EaeI 119 TGGCCA [CT]^GGCC[GA]
EcoT14I 214 CCATGG C^C[AT][AT]GG
HapII 137 CCGG C^CGG
HhaI 966 GCGC GCG^C
HinP1I 966 GCGC G^CGC
HpaII 137 CCGG C^CGG
HphI 624 TCACC GGTGA(8/7)
MamI 838 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 119 TGGCCA TGG^CCA
MscI 119 TGGCCA TGG^CCA
MslI 733 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 137 CCGG C^CGG
MwoI 198 GCAAGACGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 136 CCCGG CC^[GC]GG
NcoI 214 CCATGG C^CATGG
PflMI 375 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 240 GCTCTTC GCTCTTC(1/4)
SmlI 1009 CTCAAG C^T[CT][GA]AG
StyI 214 CCATGG C^C[AT][AT]GG
SwaI 734 ATTTAAAT ATTT^AAAT
Tsp45I 8 GTGAC ^GT[GC]AC
Van91I 375 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 612 TCTAGA T^CTAGA
XmnI 869 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BciVI 283 GGATAC GTATCC(6/5)
1034 GTATCC
BclI 621 TGATCA T^GATCA
1053 TGATCA
BsaJI 122 CCAGGG C^C[ACGT][ACGT]GG
214 CCATGG
BsrDI 1074 CATTGC GCAATG(2/0)
166 GCAATG
BstYI 499 AGATCT [GA]^GATC[CT]
927 AGATCC
DraI 346 TTTAAA TTT^AAA
735 TTTAAA
DraII 223 AGGGCCC [GA]G^G[ACGT]CC[CT]
224 GGGCCCT
EarI 241 CTCTTC CTCTTC(1/4)
270 GAAGAG
EcoO109I 223 AGGGCCC [GA]G^G[ACGT]CC[CT]
224 GGGCCCT
FbaI 621 TGATCA T^GATCA
1053 TGATCA
Ksp632I 241 CTCTTC CTCTTC(1/4)
270 GAAGAG
MaeII 158 ACGT A^CGT
203 ACGT
MflI 499 AGATCT [GA]^GATC[CT]
927 AGATCC
NlaIV 224 GGGCCC GG[ACGT]^[ACGT]CC
371 GGTCCC
PstI 878 CTGCAG CTGCA^G
999 CTGCAG
SacI 994 GAGCTC GAGCT^C
1027 GAGCTC
SfaNI 46 GATGC GCATC(5/9)
820 GATGC
SfcI 878 CTGCAG C^T[GA][CT]AG
999 CTGCAG
SspI 421 AATATT AAT^ATT
861 AATATT
TspRI 277 CAGTG CA[GC]TG[ACGT][ACGT]^
579 CACTG
XhoII 499 AGATCT [GA]^GATC[CT]
927 AGATCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AspHI BanII BfaI BmyI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII FokI HaeIII HinfI
MaeI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII PleI
Sau3AI Sau96I ScrFI TaqI TfiI
Tru9I Tsp509I TthHB8I