Restriction map for
pF1KSDA1465
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AccIII AclI AcsI AflII AlwNI
ApoI AscI Asp700I AspI AvrII
BamHI BbvCI BciVI BclI BfrI
BglII BlnI BsaBI BseAI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
DrdI EarI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
Ksp632I MamI MfeI MroI MunI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PflFI
PmeI PshAI Psp1406I RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 217 GACGTC GACGT^C
Acc65I 1681 GGTACC G^GTACC
AccI 1054 GTCTAC GT^[AC][GT]AC
AgeI 855 ACCGGT A^CCGGT
AhdI 162 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1891 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1370 AGCGCT AGC^GCT
AseI 2206 ATTAAT AT^TAAT
AsnI 2206 ATTAAT AT^TAAT
Asp718I 1681 GGTACC G^GTACC
AspEI 162 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1726 CACGTG CAC^GTG
BfaI 40 CTAG C^TAG
BglI 1039 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 507 GCTTAGC GC^T[ACGT]AGC
BpmI 953 CTCCAG CTGGAG(16/14)
Bpu1102I 507 GCTTAGC GC^T[ACGT]AGC
BsaAI 1726 CACGTG [CT]AC^GT[GA]
BsaWI 855 ACCGGT [AT]^CCGG[AT]
BsiWI 478 CGTACG C^GTACG
BsmBI 1294 GAGACG CGTCTC(1/5)
BsmI 1277 GCATTC GAATGC(1/-1)
BsrBI 864 GAGCGG CCGCTC(-3/-3)
BstF5I 1473 GGATG GGATG(2/0)
Bsu36I 1891 CCTCAGG CC^T[ACGT]AGG
CelII 507 GCTTAGC GC^T[ACGT]AGC
DraIII 425 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1675 CGGCCG C^GGCCG
Eam1105I 162 GACGCTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1675 CGGCCG C^GGCCG
Eco47III 1370 AGCGCT AGC^GCT
Eco52I 1675 CGGCCG C^GGCCG
Eco81I 1891 CCTCAGG CC^T[ACGT]AGG
EcoNI 1263 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI 1473 GGATG GGATG(9/13)
HphI 93 TCACC GGTGA(8/7)
KpnI 1681 GGTACC GGTAC^C
KspI 1665 CCGCGG CCGC^GG
MaeI 40 CTAG C^TAG
MseI 2207 TTAA T^TAA
NaeI 969 GCCGGC GCC^GGC
NgoMIV 969 GCCGGC G^CCGGC
NlaIII 56 CATG CATG^
PaeR7I 1954 CTCGAG C^TCGAG
PinAI 855 ACCGGT A^CCGGT
PmaCI 1726 CACGTG CAC^GTG
PmlI 1726 CACGTG CAC^GTG
PshBI 2206 ATTAAT AT^TAAT
PvuI 2160 CGATCG CGAT^CG
SacII 1665 CCGCGG CCGC^GG
Tru9I 2207 TTAA T^TAA
XhoI 1954 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AflIII 1104 ACGCGT A^C[GA][CT]GT
1412 ACGCGT
Alw44I 689 GTGCAC G^TGCAC
1067 GTGCAC
ApaLI 689 GTGCAC G^TGCAC
1067 GTGCAC
BalI 1265 TGGCCA TGG^CCA
1808 TGGCCA
BcgI 618 CGACTCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1935 CGACCCGCGTGC
BsaI 1288 GAGACC GGTCTC(1/5)
2051 GAGACC
BsiEI 1675 CGGCCG CG[GA][CT]^CG
2160 CGATCG
BsrDI 624 CATTGC GCAATG(2/0)
810 GCAATG
BstAPI 424 GCACTCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
651 GCAGGGGGTGC
BstYI 824 AGATCC [GA]^GATC[CT]
1343 AGATCC
HincII 1648 GTCAAC GT[CT]^[GA]AC
2188 GTCAAC
HindII 1648 GTCAAC GT[CT]^[GA]AC
2188 GTCAAC
MflI 824 AGATCC [GA]^GATC[CT]
1343 AGATCC
MluI 1104 ACGCGT A^CGCGT
1412 ACGCGT
MluNI 1265 TGGCCA TGG^CCA
1808 TGGCCA
MscI 1265 TGGCCA TGG^CCA
1808 TGGCCA
MslI 594 CACCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
706 CATCTGAGTG
PflMI 1257 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1842 CCATCTGCTGG
PpuMI 1385 GGGACCC [GA]G^G[AT]CC[CT]
2063 GGGACCT
PvuII 376 CAGCTG CAG^CTG
1501 CAGCTG
SacI 1432 GAGCTC GAGCT^C
1756 GAGCTC
StuI 578 AGGCCT AGG^CCT
1260 AGGCCT
Van91I 1257 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1842 CCATCTGCTGG
XcmI 955 CCAGCACCCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2199 CCAGGAGATTAATGG
XhoII 824 AGATCC [GA]^GATC[CT]
1343 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
AviII BanI BanII BbeI BbsI
BbvI BcnI BmyI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssHII BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FspI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI KasI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmaI SmlI StyI TaqI
TseI Tsp45I TspRI TthHB8I XmaCI
XmaI