Restriction map for
pF1KSDA1470
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrDI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SmlI SnaBI SphI
Sse8387I SspI StuI SwaI Tru9I
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Aor51HI 119 AGCGCT AGC^GCT
Asp700I 916 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 935 TGGCCA TGG^CCA
BglII 1163 AGATCT A^GATCT
BsaI 459 GGTCTC GGTCTC(1/5)
BsmBI 998 GAGACG CGTCTC(1/5)
Bsp1407I 749 TGTACA T^GTACA
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BsrGI 749 TGTACA T^GTACA
BssHII 201 GCGCGC G^CGCGC
BssSI 468 CTCGTG CACGAG(-5/-1)
BstXI 417 CCACAGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1163 AGATCT [GA]^GATC[CT]
DraIII 601 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1314 GAAGAG CTCTTC(1/4)
Eco47III 119 AGCGCT AGC^GCT
Ksp632I 1314 GAAGAG CTCTTC(1/4)
MflI 1163 AGATCT [GA]^GATC[CT]
MluNI 935 TGGCCA TGG^CCA
MscI 935 TGGCCA TGG^CCA
NcoI 1469 CCATGG C^CATGG
NspI 622 GCATGT [GA]CATG^[CT]
PpuMI 1274 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 453 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1314 GAAGAGC GCTCTTC(1/4)
SfaNI 1322 GCATC GCATC(5/9)
SfiI 1078 GGCCGGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 887 CCCGGG CCC^GGG
SpeI 918 ACTAGT A^CTAGT
SspBI 749 TGTACA T^GTACA
XcmI 1161 CCAGATCTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1163 AGATCT [GA]^GATC[CT]
XmaCI 887 CCCGGG C^CCGGG
XmaI 887 CCCGGG C^CCGGG
XmnI 916 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 719 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
967 CAGATTCTG
BanI 428 GGTGCC G^G[CT][GA]CC
836 GGTGCC
BfaI 578 CTAG C^TAG
919 CTAG
BpmI 169 CTGGAG CTGGAG(16/14)
1287 CTGGAG
BseRI 487 CTCCTC GAGGAG(10/8)
34 GAGGAG
BsgI 476 CTGCAC GTGCAG(16/14)
1270 GTGCAG
BsiEI 211 CGGCCG CG[GA][CT]^CG
242 CGGCCG
BspMI 1180 ACCTGC ACCTGC(4/8)
1461 GCAGGT
BsrFI 213 GCCGGC [GA]^CCGG[CT]
1079 GCCGGC
BsrI 338 ACTGG ACTGG(1/-1)
1383 ACTGG
BstF5I 1104 GGATG GGATG(2/0)
1443 GGATG
Cfr10I 213 GCCGGC [GA]^CCGG[CT]
1079 GCCGGC
DpnII 1164 GATC ^GATC
1530 GATC
EagI 211 CGGCCG C^GGCCG
242 CGGCCG
EclXI 211 CGGCCG C^GGCCG
242 CGGCCG
Eco52I 211 CGGCCG C^GGCCG
242 CGGCCG
EcoRI 790 GAATTC G^AATTC
1292 GAATTC
FokI 1104 GGATG GGATG(9/13)
1443 GGATG
HaeII 119 AGCGCT [GA]GCGC^[CT]
1012 GGCGCT
HgaI 721 GACGC GACGC(5/10)
281 GCGTC
MaeI 578 CTAG C^TAG
919 CTAG
MaeII 986 ACGT A^CGT
1001 ACGT
MboI 1164 GATC ^GATC
1530 GATC
MslI 418 CACAGATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1323 CATCATTGTG
NaeI 213 GCCGGC GCC^GGC
1079 GCCGGC
NdeII 1164 GATC ^GATC
1530 GATC
NgoMIV 213 GCCGGC G^CCGGC
1079 GCCGGC
SacI 42 GAGCTC GAGCT^C
172 GAGCTC
Sau3AI 1164 GATC ^GATC
1530 GATC
TfiI 969 GATTC G^A[AT]TC
1246 GAATC
Tsp45I 554 GTGAC ^GT[GC]AC
1365 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApaI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BglI
BmyI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PflMI
PleI PstI PvuII RsaI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp509I TspRI TthHB8I Van91I