Restriction map for
pF1KSDA1473
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI AlwI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaII
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr13I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MslI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI Sse8387I SspBI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Alw44I 277 GTGCAC G^TGCAC
ApaLI 277 GTGCAC G^TGCAC
AspHI 277 GTGCAC G[AT]GC[AT]^C
AvaI 149 CCCGAG C^[CT]CG[GA]G
BalI 160 TGGCCA TGG^CCA
BbsI 25 GTCTTC GAAGAC(2/6)
BbvI 40 GCTGC GCAGC(8/12)
BclI 59 TGATCA T^GATCA
BmyI 277 GTGCAC G[AGT]GC[ACT]^C
BpuAI 25 GTCTTC GAAGAC(2/6)
BsaJI 88 CCTTGG C^C[ACGT][ACGT]GG
BsgI 806 CTGCAC GTGCAG(16/14)
BsiHKAI 277 GTGCAC G[AT]GC[AT]^C
BsoBI 149 CCCGAG C^[CT]CG[GA]G
Bsp1286I 277 GTGCAC G[AGT]GC[ACT]^C
BsrDI 1548 CATTGC GCAATG(2/0)
BssKI 21 CCTGG ^CC[ACGT]GG
BstNI 21 CCTGG CC^[AT]GG
Cac8I 452 GCATGC GC[ACGT]^[ACGT]GC
DpnII 60 GATC ^GATC
DraI 1036 TTTAAA TTT^AAA
EaeI 160 TGGCCA [CT]^GGCC[GA]
EcoRII 21 CCTGG ^CC[AT]GG
EcoT14I 88 CCTTGG C^C[AT][AT]GG
FbaI 59 TGATCA T^GATCA
Fnu4HI 40 GCTGC GC^[ACGT]GC
ItaI 40 GCTGC GC^[ACGT]GC
MboI 60 GATC ^GATC
MluNI 160 TGGCCA TGG^CCA
MscI 160 TGGCCA TGG^CCA
MvaI 21 CCTGG CC^[AT]GG
NdeII 60 GATC ^GATC
NspI 452 GCATGC [GA]CATG^[CT]
Sau3AI 60 GATC ^GATC
ScrFI 21 CCTGG CC^[ACGT]GG
SexAI 20 ACCTGGT A^CC[AT]GGT
SfcI 12 CTACAG C^T[GA][CT]AG
SphI 452 GCATGC GCATG^C
SspI 333 AATATT AAT^ATT
StuI 947 AGGCCT AGG^CCT
StyI 88 CCTTGG C^C[AT][AT]GG
TseI 40 GCTGC G^C[AT]GC
TspRI 307 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 414 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
986 GAATTCATTC
BsaI 152 GAGACC GGTCTC(1/5)
538 GAGACC
BseRI 1464 CTCCTC GAGGAG(10/8)
682 GAGGAG
BsrFI 620 ACCGGC [GA]^CCGG[CT]
1124 GCCGGT
BstF5I 223 GGATG GGATG(2/0)
264 GGATG
Cfr10I 620 ACCGGC [GA]^CCGG[CT]
1124 GCCGGT
EarI 99 GAAGAG CTCTTC(1/4)
598 GAAGAG
FokI 223 GGATG GGATG(9/13)
264 GGATG
HaeIII 161 GGCC GG^CC
948 GGCC
HapII 621 CCGG C^CGG
1125 CCGG
HinfI 1407 GATTC G^A[ACGT]TC
1491 GATTC
HpaII 621 CCGG C^CGG
1125 CCGG
HphI 1585 GGTGA GGTGA(8/7)
62 TCACC
Ksp632I 99 GAAGAG CTCTTC(1/4)
598 GAAGAG
MspI 621 CCGG C^CGG
1125 CCGG
TfiI 1407 GATTC G^A[AT]TC
1491 GATTC
XmnI 414 GAAATCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
986 GAATTCATTC
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# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AcsI AluI ApoI BpmI BsiYI
BslI BsmAI BsrI DdeI EcoRI
HindIII MaeIII MboII MnlI MseI
NlaIII Tru9I Tsp45I Tsp509I