################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AviII BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlpI Bpu1102I BsaAI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstPI Bsu36I CelII CfoI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FseI FspI HaeII HhaI HinP1I KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SacII ScaI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI SspBI SwaI XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 409 GGTACC G^GTACC AccII 760 CGCG CG^CG Asp718I 409 GGTACC G^GTACC AspI 2946 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1244 CTCGGG C^[CT]CG[GA]G AvrII 2926 CCTAGG C^CTAGG BanI 409 GGTACC G^G[CT][GA]CC BanII 1225 GAGCCC G[GA]GC[CT]^C BclI 2382 TGATCA T^GATCA BlnI 2926 CCTAGG C^CTAGG BmyI 1225 GAGCCC G[AGT]GC[ACT]^C BsaBI 1477 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1969 GGTCTC GGTCTC(1/5) BsaWI 123 TCCGGT [AT]^CCGG[AT] BsgI 1428 GTGCAG GTGCAG(16/14) BsoBI 1244 CTCGGG C^[CT]CG[GA]G Bsp1286I 1225 GAGCCC G[AGT]GC[ACT]^C BspLU11I 1877 ACATGT A^CATGT BsrFI 2420 ACCGGC [GA]^CCGG[CT] BssSI 1583 CTCGTG CACGAG(-5/-1) Bst1107I 1861 GTATAC GTA^TAC BstAPI 1840 GCATTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 760 CGCG CG^CG BstZ17I 1861 GTATAC GTA^TAC Cfr10I 2420 ACCGGC [GA]^CCGG[CT] EcoNI 2717 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1711 GAATTC G^AATTC EcoT22I 416 ATGCAT ATGCA^T FbaI 2382 TGATCA T^GATCA HpaI 1650 GTTAAC GTT^AAC KpnI 409 GGTACC GGTAC^C MamI 1477 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 760 CGCG CG^CG NdeI 771 CATATG CA^TATG NsiI 416 ATGCAT ATGCA^T NspI 1877 ACATGT [GA]CATG^[CT] PflFI 2946 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1242 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 178 CAGCTG CAG^CTG SalI 97 GTCGAC G^TCGAC SbfI 2166 CCTGCAGG CCTGCA^GG SgrAI 2419 CACCGGCG C[GA]^CCGG[CT]G Sse8387I 2166 CCTGCAGG CCTGCA^GG SspI 2005 AATATT AAT^ATT StuI 1096 AGGCCT AGG^CCT Tth111I 2946 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 137 GACGCC G[GA]^CG[CT]C 2946 GACGCC AflIII 1877 ACATGT A^C[GA][CT]GT 2327 ACACGT BalI 575 TGGCCA TGG^CCA 2202 TGGCCA BamHI 1615 GGATCC G^GATCC 2541 GGATCC BcnI 1155 CCCGG CC^[GC]GG 2586 CCGGG BglI 140 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 556 GCCACAGTGGC BsaHI 137 GACGCC G[GA]^CG[CT]C 2946 GACGCC BspDI 1147 ATCGAT AT^CGAT 2553 ATCGAT BspMI 2033 ACCTGC ACCTGC(4/8) 1849 GCAGGT ClaI 1147 ATCGAT AT^CGAT 2553 ATCGAT DraII 803 GGGTCCT [GA]G^G[ACGT]CC[CT] 2564 AGGTCCC DrdI 1450 GACAAAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1978 GACAGCCCTGTC EcoO109I 803 GGGTCCT [GA]G^G[ACGT]CC[CT] 2564 AGGTCCC HgaI 137 GACGC GACGC(5/10) 2946 GACGC Hin1I 137 GACGCC G[GA]^CG[CT]C 2946 GACGCC HincII 97 GTCGAC GT[CT]^[GA]AC 1650 GTTAAC HindII 97 GTCGAC GT[CT]^[GA]AC 1650 GTTAAC HindIII 1130 AAGCTT A^AGCTT 2368 AAGCTT MaeII 2329 ACGT A^CGT 2966 ACGT MluNI 575 TGGCCA TGG^CCA 2202 TGGCCA MscI 575 TGGCCA TGG^CCA 2202 TGGCCA MspA1I 178 CAGCTG C[AC]G^C[GT]G 799 CAGCGG NciI 1155 CCCGG CC^[GC]GG 2586 CCGGG PflMI 1093 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2059 CCACCCAATGG PpuMI 803 GGGTCCT [GA]G^G[AT]CC[CT] 2564 AGGTCCC SapI 2178 GAAGAGC GCTCTTC(1/4) 2122 GCTCTTC Tsp45I 1586 GTGAC ^GT[GC]AC 1630 GTGAC Van91I 1093 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2059 CCACCCAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BbsI BbvI BfaI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsmI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII