Restriction map for
pF1KSDA1486
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I AlwI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BssHII Bst1107I BstAPI
BstBI BstXI BstYI BstZ17I ClaI
CpoI DraI DraIII Eam1105I Eco47III
EcoNI EcoRI EcoT22I FbaI FseI
HpaI KasI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SwaI Tru9I Tth111I
Van91I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1932 GGTACC G^GTACC
AflIII 1103 ACGTGT A^C[GA][CT]GT
ApaI 646 GGGCCC GGGCC^C
Asp718I 1932 GGTACC G^GTACC
AviII 1574 TGCGCA TGC^GCA
BcgI 611 GCACATCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 259 TACGTG [CT]AC^GT[GA]
BsaI 707 GAGACC GGTCTC(1/5)
BsgI 361 CTGCAC GTGCAG(16/14)
BsiEI 1560 CGGCCG CG[GA][CT]^CG
Bsp1407I 731 TGTACA T^GTACA
BspHI 287 TCATGA T^CATGA
BsrBI 495 GAGCGG CCGCTC(-3/-3)
BsrDI 1033 CATTGC GCAATG(2/0)
BsrGI 731 TGTACA T^GTACA
BssSI 1521 CACGAG CACGAG(-5/-1)
BstEII 1764 GGTCACC G^GT[ACGT]ACC
BstPI 1764 GGTCACC G^GT[ACGT]ACC
DrdI 516 GACTGAAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1560 CGGCCG C^GGCCG
EclXI 1560 CGGCCG C^GGCCG
Eco52I 1560 CGGCCG C^GGCCG
EcoO65I 1764 GGTCACC G^GT[ACGT]ACC
EcoRV 3 GATATC GAT^ATC
FspI 1574 TGCGCA TGC^GCA
HaeII 1595 GGCGCT [GA]GCGC^[CT]
HindIII 1084 AAGCTT A^AGCTT
KpnI 1932 GGTACC GGTAC^C
NaeI 1592 GCCGGC GCC^GGC
NgoMIV 1592 GCCGGC G^CCGGC
PaeR7I 128 CTCGAG C^TCGAG
PshAI 1433 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 287 TCATGA T^CATGA
SacI 1401 GAGCTC GAGCT^C
SfaNI 1784 GATGC GCATC(5/9)
SmaI 1494 CCCGGG CCC^GGG
SspBI 731 TGTACA T^GTACA
SspI 554 AATATT AAT^ATT
Tsp509I 1847 AATT ^AATT
XhoI 128 CTCGAG C^TCGAG
XmaCI 1494 CCCGGG C^CCGGG
XmaI 1494 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AatII 657 GACGTC GACGT^C
1866 GACGTC
AlwNI 355 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
390 CAGCAGCTG
BanI 900 GGTGCC G^G[CT][GA]CC
1932 GGTACC
BfaI 163 CTAG C^TAG
1777 CTAG
BlpI 450 GCTGAGC GC^T[ACGT]AGC
606 GCTGAGC
Bpu1102I 450 GCTGAGC GC^T[ACGT]AGC
606 GCTGAGC
BsmI 111 GAATGC GAATGC(1/-1)
1380 GCATTC
BsrFI 1592 GCCGGC [GA]^CCGG[CT]
1853 GCCGGT
CelII 450 GCTGAGC GC^T[ACGT]AGC
606 GCTGAGC
Cfr10I 1592 GCCGGC [GA]^CCGG[CT]
1853 GCCGGT
DpnII 139 GATC ^GATC
350 GATC
EarI 1549 CTCTTC CTCTTC(1/4)
718 GAAGAG
HphI 1473 GGTGA GGTGA(8/7)
1766 TCACC
Ksp632I 1549 CTCTTC CTCTTC(1/4)
718 GAAGAG
MaeI 163 CTAG C^TAG
1777 CTAG
MboI 139 GATC ^GATC
350 GATC
NcoI 1278 CCATGG C^CATGG
1695 CCATGG
NdeII 139 GATC ^GATC
350 GATC
PstI 482 CTGCAG CTGCA^G
1610 CTGCAG
SalI 1772 GTCGAC G^TCGAC
1863 GTCGAC
Sau3AI 139 GATC ^GATC
350 GATC
SfcI 482 CTGCAG C^T[GA][CT]AG
1610 CTGCAG
TfiI 284 GATTC G^A[AT]TC
567 GATTC
XcmI 1269 CCAGTCTCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1520 CCACGAGCCCGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AocI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI Bsu36I Cac8I
CfoI Cfr13I DdeI DraII DsaI
EaeI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII ItaI KspI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV PleI PpuMI
PvuII RsaI SacII Sau96I ScrFI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I