Restriction map for
pF1KSDA1490
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII AvrII BbeI BbrPI
BbsI BciVI BclI BfrI BglII
BlnI BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CfoI ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SmaI SnaBI SpeI
Sse8387I SspBI StuI SwaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AfaI 1648 GTAC GT^AC
AlwNI 5 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 57 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1063 GCTGAGG CCTCAGC(-5/-2)
BcgI 702 CGACTATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1679 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 583 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 583 GCTGAGC GC^T[ACGT]AGC
BsaAI 361 TACGTG [CT]AC^GT[GA]
BsgI 671 CTGCAC GTGCAG(16/14)
BsmBI 74 CGTCTC CGTCTC(1/5)
BssSI 300 CACGAG CACGAG(-5/-1)
BstAPI 36 GCACATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 583 GCTGAGC GC^T[ACGT]AGC
DsaI 2042 CCATGG C^C[GA][CT]GG
EcoNI 111 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 97 GCGGG CCCGC(4/6)
HindIII 919 AAGCTT A^AGCTT
MaeII 362 ACGT A^CGT
MfeI 1760 CAATTG C^AATTG
MslI 1624 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1760 CAATTG C^AATTG
NcoI 2042 CCATGG C^CATGG
PflMI 1589 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 1061 CAGCTG CAG^CTG
RsaI 1648 GTAC GT^AC
ScaI 1647 AGTACT AGT^ACT
SgrAI 84 CACCGGCG C[GA]^CCGG[CT]G
SmlI 968 CTCAAG C^T[CT][GA]AG
SphI 1184 GCATGC GCATG^C
Van91I 1589 CCAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1290 TCTAGA T^CTAGA
XmnI 57 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AcsI 559 GAATTC [GA]^AATT[CT]
947 GAATTC
AflIII 1883 ACATGT A^C[GA][CT]GT
1930 ACATGT
ApoI 559 GAATTC [GA]^AATT[CT]
947 GAATTC
AspI 793 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1628 GACATGGTC
BalI 1085 TGGCCA TGG^CCA
1925 TGGCCA
BamHI 1457 GGATCC G^GATCC
1719 GGATCC
BcnI 93 CCCGG CC^[GC]GG
1991 CCCGG
BpmI 543 CTCCAG CTGGAG(16/14)
1689 CTGGAG
BsmFI 147 GGGAC GGGAC(10/14)
791 GGGAC
BspHI 914 TCATGA T^CATGA
1962 TCATGA
BspLU11I 1883 ACATGT A^CATGT
1930 ACATGT
BspMI 669 ACCTGC ACCTGC(4/8)
2212 GCAGGT
BsrDI 1172 GCAATG GCAATG(2/0)
1297 GCAATG
BsrFI 85 ACCGGC [GA]^CCGG[CT]
508 ACCGGC
BstYI 1457 GGATCC [GA]^GATC[CT]
1719 GGATCC
Cfr10I 85 ACCGGC [GA]^CCGG[CT]
508 ACCGGC
EcoRI 559 GAATTC G^AATTC
947 GAATTC
EcoT14I 2042 CCATGG C^C[AT][AT]GG
2091 CCTTGG
HincII 1617 GTCAAC GT[CT]^[GA]AC
1730 GTCAAC
HindII 1617 GTCAAC GT[CT]^[GA]AC
1730 GTCAAC
HphI 478 GGTGA GGTGA(8/7)
2095 GGTGA
MflI 1457 GGATCC [GA]^GATC[CT]
1719 GGATCC
MluNI 1085 TGGCCA TGG^CCA
1925 TGGCCA
MscI 1085 TGGCCA TGG^CCA
1925 TGGCCA
NciI 93 CCCGG CC^[GC]GG
1991 CCCGG
PflFI 793 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1628 GACATGGTC
PpuMI 147 GGGACCC [GA]G^G[AT]CC[CT]
791 GGGACCT
RcaI 914 TCATGA T^CATGA
1962 TCATGA
SacI 439 GAGCTC GAGCT^C
1069 GAGCTC
SspI 1853 AATATT AAT^ATT
2200 AATATT
StyI 2042 CCATGG C^C[AT][AT]GG
2091 CCTTGG
TfiI 1287 GATTC G^A[AT]TC
2152 GAATC
Tth111I 793 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1628 GACATGGTC
XhoII 1457 GGATCC [GA]^GATC[CT]
1719 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BanI BanII BbvI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT22I Fnu4HI FokI HaeIII HapII
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I