Restriction map for
pF1KSDA1498
##################################
# NO CUT ENZYMES ( 49 enzymes) #
##################################
Acc65I AgeI AocI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BamHI BbrPI BlnI BspHI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I CpoI
Eco81I EcoRI EcoRV EcoT22I FspI
KpnI NdeI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI RcaI RsrII SalI
ScaI SfiI SfuI SnaBI SpeI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AccI 6349 GTCTAC GT^[AC][GT]AC
AclI 3371 AACGTT AA^CGTT
AflII 8701 CTTAAG C^TTAAG
AflIII 7130 ACGCGT A^C[GA][CT]GT
Aor51HI 319 AGCGCT AGC^GCT
BclI 7239 TGATCA T^GATCA
BfrI 8701 CTTAAG C^TTAAG
BsaBI 3030 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 6979 CGTACG C^GTACG
BspDI 5479 ATCGAT AT^CGAT
BssHII 8663 GCGCGC G^CGCGC
ClaI 5479 ATCGAT AT^CGAT
DraI 6147 TTTAAA TTT^AAA
DrdI 8982 GACCACGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 319 AGCGCT AGC^GCT
FbaI 7239 TGATCA T^GATCA
FseI 741 GGCCGGCC GGCCGG^CC
HindIII 5351 AAGCTT A^AGCTT
HpaI 6871 GTTAAC GTT^AAC
MamI 3030 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 7130 ACGCGT A^CGCGT
NotI 5193 GCGGCCGC GC^GGCCGC
PshAI 6372 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3371 AACGTT AA^CGTT
PvuI 8483 CGATCG CGAT^CG
SbfI 6258 CCTGCAGG CCTGCA^GG
SgrAI 4520 CGCCGGTG C[GA]^CCGG[CT]G
SphI 7520 GCATGC GCATG^C
Sse8387I 6258 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccIII 1074 TCCGGA T^CCGGA
4434 TCCGGA
AcsI 2604 AAATTT [GA]^AATT[CT]
6629 AAATTC
ApoI 2604 AAATTT [GA]^AATT[CT]
6629 AAATTC
BbeI 2866 GGCGCC GGCGC^C
4711 GGCGCC
BcgI 5481 CGATGAAAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
136 GCAAGCCCGTCG
BciVI 2196 GGATAC GTATCC(6/5)
4059 GTATCC
BsaAI 4039 TACGTG [CT]AC^GT[GA]
6499 TACGTG
BseAI 1074 TCCGGA T^CCGGA
4434 TCCGGA
BsmBI 1384 GAGACG CGTCTC(1/5)
4516 GAGACG
Bsp1407I 5207 TGTACA T^GTACA
7028 TGTACA
BspEI 1074 TCCGGA T^CCGGA
4434 TCCGGA
BsrGI 5207 TGTACA T^GTACA
7028 TGTACA
BstEII 1465 GGTCACC G^GT[ACGT]ACC
8979 GGTGACC
BstPI 1465 GGTCACC G^GT[ACGT]ACC
8979 GGTGACC
DraIII 565 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
3781 CACAGGGTG
EcoNI 3093 CCTGCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5592 CCTTGTAGAGG
EcoO65I 1465 GGTCACC G^GT[ACGT]ACC
8979 GGTGACC
KasI 2866 GGCGCC G^GCGCC
4711 GGCGCC
MfeI 2756 CAATTG C^AATTG
6131 CAATTG
MroI 1074 TCCGGA T^CCGGA
4434 TCCGGA
MunI 2756 CAATTG C^AATTG
6131 CAATTG
NarI 2866 GGCGCC GG^CGCC
4711 GGCGCC
NheI 2710 GCTAGC G^CTAGC
6102 GCTAGC
SexAI 2228 ACCAGGT A^CC[AT]GGT
7727 ACCTGGT
SfoI 2866 GGCGCC GGC^GCC
4711 GGCGCC
SspBI 5207 TGTACA T^GTACA
7028 TGTACA
SspI 2539 AATATT AAT^ATT
6661 AATATT
XcmI 698 CCATCCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
8291 CCAACCTCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 152 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AhdI AluI Alw44I AlwI AlwNI
ApaI ApaLI AspEI AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SacI SacII SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XhoII
XmaCI XmaI