Restriction map for
pF1KSDA1537
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsoBI BspEI BspLU11I BspMI BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstZ17I Bsu36I Cfr10I
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoT14I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 51 CCGC CCGC(-3/-1)
AlwNI 1549 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 168 GGGCCC GGGCC^C
Asp700I 1333 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 168 GGGCCC G[GA]GC[CT]^C
BcnI 228 CCGGG CC^[GC]GG
BglII 1335 AGATCT A^GATCT
BlpI 1494 GCTTAGC GC^T[ACGT]AGC
BmyI 168 GGGCCC G[AGT]GC[ACT]^C
Bpu1102I 1494 GCTTAGC GC^T[ACGT]AGC
BsiYI 51 CCGCACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 51 CCGCACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1551 GGGAC GGGAC(10/14)
BsmI 816 GCATTC GAATGC(1/-1)
Bsp1286I 168 GGGCCC G[AGT]GC[ACT]^C
Bsp1407I 842 TGTACA T^GTACA
BspDI 430 ATCGAT AT^CGAT
BspHI 1455 TCATGA T^CATGA
BsrGI 842 TGTACA T^GTACA
BstAPI 1320 GCAGACTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 774 GGTTACC G^GT[ACGT]ACC
BstPI 774 GGTTACC G^GT[ACGT]ACC
CelII 1494 GCTTAGC GC^T[ACGT]AGC
CfoI 1177 GCGC GCG^C
ClaI 430 ATCGAT AT^CGAT
DraI 501 TTTAAA TTT^AAA
EcoO65I 774 GGTTACC G^GT[ACGT]ACC
EcoRI 1606 GAATTC G^AATTC
EcoRV 967 GATATC GAT^ATC
EcoT22I 1733 ATGCAT ATGCA^T
HapII 228 CCGG C^CGG
HhaI 1177 GCGC GCG^C
HinP1I 1177 GCGC G^CGC
HindIII 1492 AAGCTT A^AGCTT
HpaII 228 CCGG C^CGG
HphI 1699 TCACC GGTGA(8/7)
MaeII 318 ACGT A^CGT
MslI 1035 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 228 CCGG C^CGG
NciI 228 CCGGG CC^[GC]GG
NheI 906 GCTAGC G^CTAGC
NsiI 1733 ATGCAT ATGCA^T
RcaI 1455 TCATGA T^CATGA
SapI 1471 GCTCTTC GCTCTTC(1/4)
SexAI 237 ACCTGGT A^CC[AT]GGT
SmlI 345 CTTGAG C^T[CT][GA]AG
SspBI 842 TGTACA T^GTACA
StuI 996 AGGCCT AGG^CCT
TspRI 1633 CACTG CA[GC]TG[ACGT][ACGT]^
XmnI 1333 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 1606 GAATTC [GA]^AATT[CT]
1652 AAATTT
AlwI 231 GGATC GGATC(4/5)
339 GGATC
ApoI 1606 GAATTC [GA]^AATT[CT]
1652 AAATTT
BbsI 246 GAAGAC GAAGAC(2/6)
1519 GAAGAC
BpuAI 246 GAAGAC GAAGAC(2/6)
1519 GAAGAC
BsmAI 1378 GAGAC GTCTC(1/5)
1223 GTCTC
Cfr13I 168 GGGCC G^G[ACGT]CC
169 GGCCC
DraII 167 GGGGCCC [GA]G^G[ACGT]CC[CT]
168 GGGCCCT
EaeI 48 TGGCCG [CT]^GGCC[GA]
305 TGGCCG
EarI 1472 CTCTTC CTCTTC(1/4)
667 GAAGAG
EcoO109I 167 GGGGCCC [GA]G^G[ACGT]CC[CT]
168 GGGCCCT
Ksp632I 1472 CTCTTC CTCTTC(1/4)
667 GAAGAG
MspA1I 1144 CAGCTG C[AC]G^C[GT]G
1273 CAGCTG
NlaIV 167 GGGGCC GG[ACGT]^[ACGT]CC
168 GGGCCC
PstI 1130 CTGCAG CTGCA^G
1243 CTGCAG
PvuII 1144 CAGCTG CAG^CTG
1273 CAGCTG
Sau96I 168 GGGCC G^G[ACGT]CC
169 GGCCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AfaI AluI BbvI BfaI BpmI
BsaBI BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TthHB8I
XcmI XhoII