Restriction map for
pF1KSDA1538
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
AhdI Alw44I AlwI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BcgI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BseAI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BssHII BstBI
BstEII BstPI BstYI ClaI CpoI
DraI Eam1105I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HpaI KspI MaeI MamI
MfeI MflI MluI MroI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SspI StuI SwaI
TaqI Tru9I TthHB8I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 91 GACGTC GACGT^C
AcsI 2620 GAATTT [GA]^AATT[CT]
AocI 2983 CCTAAGG CC^T[ACGT]AGG
Aor51HI 2416 AGCGCT AGC^GCT
ApoI 2620 GAATTT [GA]^AATT[CT]
AspI 957 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BsaWI 2714 ACCGGA [AT]^CCGG[AT]
BsgI 2614 GTGCAG GTGCAG(16/14)
Bsp1407I 987 TGTACA T^GTACA
BsrGI 987 TGTACA T^GTACA
BssSI 993 CTCGTG CACGAG(-5/-1)
Bst1107I 2602 GTATAC GTA^TAC
BstAPI 2929 GCACCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2602 GTATAC GTA^TAC
Bsu36I 2983 CCTAAGG CC^T[ACGT]AGG
DpnII 1809 GATC ^GATC
DraIII 892 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 21 GACGGACCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2416 AGCGCT AGC^GCT
Eco81I 2983 CCTAAGG CC^T[ACGT]AGG
EcoNI 531 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MboI 1809 GATC ^GATC
MslI 979 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 2078 GCCGGC GCC^GGC
NdeII 1809 GATC ^GATC
NgoMIV 2078 GCCGGC G^CCGGC
NspI 2358 GCATGC [GA]CATG^[CT]
PflFI 957 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI 1809 GATC ^GATC
SbfI 832 CCTGCAGG CCTGCA^GG
SphI 2358 GCATGC GCATG^C
Sse8387I 832 CCTGCAGG CCTGCA^GG
SspBI 987 TGTACA T^GTACA
Tth111I 957 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 625 CCAGCTGGGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 555 GGTACC G^GTACC
1091 GGTACC
AccI 792 GTCTAC GT^[AC][GT]AC
2602 GTATAC
AccII 785 CGCG CG^CG
933 CGCG
Asp718I 555 GGTACC G^GTACC
1091 GGTACC
BalI 578 TGGCCA TGG^CCA
1649 TGGCCA
BbeI 217 GGCGCC GGCGC^C
500 GGCGCC
BbrPI 892 CACGTG CAC^GTG
916 CACGTG
BlpI 1881 GCTGAGC GC^T[ACGT]AGC
2365 GCTGAGC
Bpu1102I 1881 GCTGAGC GC^T[ACGT]AGC
2365 GCTGAGC
BsaI 1114 GAGACC GGTCTC(1/5)
2527 GAGACC
BsiEI 2135 CGGCCG CG[GA][CT]^CG
2646 CGGCCG
BsrFI 1335 ACCGGC [GA]^CCGG[CT]
2078 GCCGGC
BstUI 785 CGCG CG^CG
933 CGCG
BstXI 1715 CCAAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2083 CCACCACGTTGG
CelII 1881 GCTGAGC GC^T[ACGT]AGC
2365 GCTGAGC
Cfr10I 1335 ACCGGC [GA]^CCGG[CT]
2078 GCCGGC
EagI 2135 CGGCCG C^GGCCG
2646 CGGCCG
EclXI 2135 CGGCCG C^GGCCG
2646 CGGCCG
Eco52I 2135 CGGCCG C^GGCCG
2646 CGGCCG
HindIII 723 AAGCTT A^AGCTT
2519 AAGCTT
KasI 217 GGCGCC G^GCGCC
500 GGCGCC
KpnI 555 GGTACC GGTAC^C
1091 GGTACC
MluNI 578 TGGCCA TGG^CCA
1649 TGGCCA
MscI 578 TGGCCA TGG^CCA
1649 TGGCCA
MvnI 785 CGCG CG^CG
933 CGCG
NarI 217 GGCGCC GG^CGCC
500 GGCGCC
PleI 1698 GACTC GAGTC(4/5)
389 GAGTC
PmaCI 892 CACGTG CAC^GTG
916 CACGTG
PmlI 892 CACGTG CAC^GTG
916 CACGTG
PshAI 1116 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1864 GACCTGCGTC
SfoI 217 GGCGCC GGC^GCC
500 GGCGCC
SmlI 1234 CTCAAG C^T[CT][GA]AG
2921 CTCAAG
Tsp509I 2621 AATT ^AATT
2976 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MwoI NciI NcoI NlaIII NlaIV
PflMI PpuMI PstI PvuII RsaI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TfiI TseI Tsp45I TspRI
Van91I XmaCI XmaI