Restriction map for
pF1KSDA1540
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MaeI MfeI MluI
MroI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 84 CGCG CG^CG
AlwNI 1008 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1105 CCTCAGG CC^T[ACGT]AGG
BclI 356 TGATCA T^GATCA
BpmI 1216 CTCCAG CTGGAG(16/14)
BsaBI 540 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 463 CTGCAC GTGCAG(16/14)
BsmBI 468 CGTCTC CGTCTC(1/5)
BsmI 302 GAATGC GAATGC(1/-1)
BspMI 1012 ACCTGC ACCTGC(4/8)
BsrDI 492 CATTGC GCAATG(2/0)
BstAPI 1007 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 84 CGCG CG^CG
BstXI 491 CCATTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 539 GGATCT [GA]^GATC[CT]
Bsu36I 1105 CCTCAGG CC^T[ACGT]AGG
DrdI 1230 GACAGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1105 CCTCAGG CC^T[ACGT]AGG
FbaI 356 TGATCA T^GATCA
HincII 331 GTCAAC GT[CT]^[GA]AC
HindII 331 GTCAAC GT[CT]^[GA]AC
HinfI 190 GAGTC G^A[ACGT]TC
MamI 540 GATCTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 539 GGATCT [GA]^GATC[CT]
MspA1I 692 CAGCTG C[AC]G^C[GT]G
MvnI 84 CGCG CG^CG
NcoI 584 CCATGG C^CATGG
PleI 190 GAGTC GAGTC(4/5)
PvuII 692 CAGCTG CAG^CTG
SexAI 509 ACCAGGT A^CC[AT]GGT
SfaNI 882 GATGC GCATC(5/9)
Tsp509I 626 AATT ^AATT
XcmI 1212 CCAACTCCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 539 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AluI 693 AGCT AG^CT
1064 AGCT
AlwI 501 GGATC GGATC(4/5)
539 GGATC
AvaII 3 GGTCC G^G[AT]CC
581 GGACC
BanII 52 GAGCCC G[GA]GC[CT]^C
1178 GGGCTC
BcnI 66 CCGGG CC^[GC]GG
536 CCCGG
BsmAI 26 GTCTC GTCTC(1/5)
469 GTCTC
BsmFI 4 GTCCC GGGAC(10/14)
192 GTCCC
BssSI 58 CACGAG CACGAG(-5/-1)
779 CTCGTG
DsaI 203 CCACGG C^C[GA][CT]GG
584 CCATGG
EarI 322 CTCTTC CTCTTC(1/4)
700 CTCTTC
EcoT14I 35 CCTTGG C^C[AT][AT]GG
584 CCATGG
HaeII 176 GGCGCT [GA]GCGC^[CT]
184 GGCGCT
HapII 66 CCGG C^CGG
537 CCGG
HpaII 66 CCGG C^CGG
537 CCGG
HphI 207 GGTGA GGTGA(8/7)
9 TCACC
Ksp632I 322 CTCTTC CTCTTC(1/4)
700 CTCTTC
MaeIII 823 GTGAC ^GT[ACGT]AC
1258 GTGAC
MspI 66 CCGG C^CGG
537 CCGG
NciI 66 CCGGG CC^[GC]GG
536 CCCGG
ScaI 452 AGTACT AGT^ACT
836 AGTACT
StyI 35 CCTTGG C^C[AT][AT]GG
584 CCATGG
Tsp45I 823 GTGAC ^GT[GC]AC
1258 GTGAC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI BalI BbsI BbvI
BmyI BpuAI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EaeI EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I ItaI MaeII
MboI MboII MluNI MnlI MscI
MslI MvaI MwoI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI TseI TspRI