Restriction map for
pF1KSDA1547
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstBI BstZ17I
Bsu36I CelII ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI Tth111I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 1680 GACGTC GACGT^C
AccI 1465 GTCTAC GT^[AC][GT]AC
AccII 328 CGCG CG^CG
AlwI 83 GGATC GGATC(4/5)
AviII 1034 TGCGCA TGC^GCA
BalI 1709 TGGCCA TGG^CCA
BbsI 159 GAAGAC GAAGAC(2/6)
BciVI 3 GTATCC GTATCC(6/5)
BpuAI 159 GAAGAC GAAGAC(2/6)
BsaI 448 GGTCTC GGTCTC(1/5)
BseRI 688 GAGGAG GAGGAG(10/8)
BsiEI 1198 CGATCG CG[GA][CT]^CG
BsrBI 1569 CCGCTC CCGCTC(-3/-3)
BsrDI 1761 CATTGC GCAATG(2/0)
BssHII 327 GCGCGC G^CGCGC
BstEII 339 GGTCACC G^GT[ACGT]ACC
BstPI 339 GGTCACC G^GT[ACGT]ACC
BstUI 328 CGCG CG^CG
BstXI 1812 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 52 TTTAAA TTT^AAA
DrdI 1723 GACGCCAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1416 CCACGG C^C[GA][CT]GG
EcoNI 1902 CCTGCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 339 GGTCACC G^GT[ACGT]ACC
EcoRI 94 GAATTC G^AATTC
FspI 1034 TGCGCA TGC^GCA
MluNI 1709 TGGCCA TGG^CCA
MscI 1709 TGGCCA TGG^CCA
MseI 53 TTAA T^TAA
MvnI 328 CGCG CG^CG
PleI 70 GAGTC GAGTC(4/5)
PvuI 1198 CGATCG CGAT^CG
StuI 1260 AGGCCT AGG^CCT
SwaI 51 ATTTAAAT ATTT^AAAT
Tru9I 53 TTAA T^TAA
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 55 AAATTC [GA]^AATT[CT]
94 GAATTC
ApoI 55 AAATTC [GA]^AATT[CT]
94 GAATTC
BcgI 1598 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2099 GCAAGTGGTTCG
BglI 862 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1508 GCCAGCCAGGC
BglII 1493 AGATCT A^GATCT
1946 AGATCT
BsmAI 249 GAGAC GTCTC(1/5)
449 GTCTC
BsrFI 1020 GCCGGC [GA]^CCGG[CT]
1132 GCCGGC
BssSI 1455 CACGAG CACGAG(-5/-1)
2059 CACGAG
BstYI 1493 AGATCT [GA]^GATC[CT]
1946 AGATCT
Cfr10I 1020 GCCGGC [GA]^CCGG[CT]
1132 GCCGGC
EaeI 1194 TGGCCG [CT]^GGCC[GA]
1709 TGGCCA
EarI 873 CTCTTC CTCTTC(1/4)
246 GAAGAG
EcoT14I 909 CCTTGG C^C[AT][AT]GG
964 CCAAGG
Ksp632I 873 CTCTTC CTCTTC(1/4)
246 GAAGAG
MflI 1493 AGATCT [GA]^GATC[CT]
1946 AGATCT
NaeI 1020 GCCGGC GCC^GGC
1132 GCCGGC
NgoMIV 1020 GCCGGC G^CCGGC
1132 GCCGGC
PflMI 1785 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1856 CCAAACTGTGG
PshAI 907 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1788 GACCCTGGTC
PstI 1178 CTGCAG CTGCA^G
1746 CTGCAG
SmaI 37 CCCGGG CCC^GGG
779 CCCGGG
StyI 909 CCTTGG C^C[AT][AT]GG
964 CCAAGG
TaqI 566 TCGA T^CGA
1015 TCGA
TthHB8I 566 TCGA T^CGA
1015 TCGA
Van91I 1785 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1856 CCAAACTGTGG
XcmI 1529 CCATCTCCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1634 CCATCTGGGACCTGG
XhoII 1493 AGATCT [GA]^GATC[CT]
1946 AGATCT
XmaCI 37 CCCGGG C^CCGGG
779 CCCGGG
XmaI 37 CCCGGG C^CCGGG
779 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BmyI BpmI
BsaHI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MaeII MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MwoI NarI NciI
NdeII NlaIII NlaIV PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI TfiI TseI
Tsp45I Tsp509I TspRI