Restriction map for
pF1KSDA1552
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvrII BalI BbrPI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StyI
SwaI Tru9I Tsp509I Tth111I XbaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflIII 2077 ACGTGT A^C[GA][CT]GT
Aor51HI 773 AGCGCT AGC^GCT
Asp700I 2047 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 166 GGATCC G^GATCC
BbeI 548 GGCGCC GGCGC^C
BfaI 286 CTAG C^TAG
BsaWI 1701 ACCGGA [AT]^CCGG[AT]
BsiEI 1589 CGGCCG CG[GA][CT]^CG
BsmAI 1042 GAGAC GTCTC(1/5)
BsmBI 1042 GAGACG CGTCTC(1/5)
Bsp1407I 392 TGTACA T^GTACA
BsrBI 2011 GAGCGG CCGCTC(-3/-3)
BsrGI 392 TGTACA T^GTACA
EagI 1589 CGGCCG C^GGCCG
EclXI 1589 CGGCCG C^GGCCG
Eco47III 773 AGCGCT AGC^GCT
Eco52I 1589 CGGCCG C^GGCCG
FseI 45 GGCCGGCC GGCCGG^CC
KasI 548 GGCGCC G^GCGCC
MaeI 286 CTAG C^TAG
NarI 548 GGCGCC GG^CGCC
NspI 444 ACATGC [GA]CATG^[CT]
PaeR7I 808 CTCGAG C^TCGAG
PflMI 1224 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1048 CTGCAG CTGCA^G
PvuII 2062 CAGCTG CAG^CTG
SfiI 616 GGCCCTGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 548 GGCGCC GGC^GCC
SmaI 1242 CCCGGG CCC^GGG
SspBI 392 TGTACA T^GTACA
TfiI 2052 GATTC G^A[AT]TC
Van91I 1224 CCAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 808 CTCGAG C^TCGAG
XmaCI 1242 CCCGGG C^CCGGG
XmaI 1242 CCCGGG C^CCGGG
XmnI 2047 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AatII 79 GACGTC GACGT^C
2119 GACGTC
Alw44I 184 GTGCAC G^TGCAC
1828 GTGCAC
ApaI 615 GGGCCC GGGCC^C
654 GGGCCC
ApaLI 184 GTGCAC G^TGCAC
1828 GTGCAC
AviII 1682 TGCGCA TGC^GCA
1955 TGCGCA
BanI 548 GGCGCC G^G[CT][GA]CC
732 GGCACC
BglI 617 GCCCTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1505 GCCCAGGAGGC
BsgI 922 CTGCAC GTGCAG(16/14)
306 GTGCAG
BssSI 850 CACGAG CACGAG(-5/-1)
1831 CACGAG
EaeI 48 CGGCCA [CT]^GGCC[GA]
1589 CGGCCG
EcoNI 579 CCTGCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
717 CCTGGAGGAGG
FspI 1682 TGCGCA TGC^GCA
1955 TGCGCA
HaeII 548 GGCGCC [GA]GCGC^[CT]
773 AGCGCT
SfcI 1048 CTGCAG C^T[GA][CT]AG
1845 CTACAG
SmlI 808 CTCGAG C^T[CT][GA]AG
1998 CTTGAG
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# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI Eco81I EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I ScrFI SfaNI StuI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII