Restriction map for
pF1KSDA1576
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# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AflIII AgeI AhdI
AlwNI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacII
SalI SbfI ScaI SexAI SfcI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 436 GTCTAC GT^[AC][GT]AC
AcyI 112 GACGCC G[GA]^CG[CT]C
Alw44I 556 GTGCAC G^TGCAC
AocI 196 CCTCAGG CC^T[ACGT]AGG
ApaLI 556 GTGCAC G^TGCAC
AspI 370 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1209 GGACC G^G[AT]CC
BanI 300 GGTGCC G^G[CT][GA]CC
BbsI 791 GTCTTC GAAGAC(2/6)
BcgI 468 CGAGGCTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 977 GCCGGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 592 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 592 GCTCAGC GC^T[ACGT]AGC
BpuAI 791 GTCTTC GAAGAC(2/6)
BsaAI 703 TACGTG [CT]AC^GT[GA]
BsaHI 112 GACGCC G[GA]^CG[CT]C
BsgI 417 CTGCAC GTGCAG(16/14)
BsiEI 1104 CGACCG CG[GA][CT]^CG
BsmBI 31 GAGACG CGTCTC(1/5)
BsmI 478 GCATTC GAATGC(1/-1)
BspMI 888 GCAGGT ACCTGC(4/8)
BsrBI 1237 GAGCGG CCGCTC(-3/-3)
BsrFI 69 GCCGGC [GA]^CCGG[CT]
BssHII 126 GCGCGC G^CGCGC
Bsu36I 196 CCTCAGG CC^T[ACGT]AGG
CelII 592 GCTCAGC GC^T[ACGT]AGC
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
DraI 954 TTTAAA TTT^AAA
Eco81I 196 CCTCAGG CC^T[ACGT]AGG
EcoT14I 5 CCAAGG C^C[AT][AT]GG
Hin1I 112 GACGCC G[GA]^CG[CT]C
MslI 420 CACACCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 69 GCCGGC GCC^GGC
NgoMIV 69 GCCGGC G^CCGGC
PflFI 370 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 208 GAGCTC GAGCT^C
SfiI 976 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 211 CTCAAG C^T[CT][GA]AG
SspI 271 AATATT AAT^ATT
StyI 5 CCAAGG C^C[AT][AT]GG
TaqI 47 TCGA T^CGA
Tth111I 370 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 47 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 216 GATCC GGATC(4/5)
444 GATCC
AvaI 106 CTCGGG C^[CT]CG[GA]G
190 CCCGAG
BalI 2 TGGCCA TGG^CCA
1163 TGGCCA
BpmI 845 CTGGAG CTGGAG(16/14)
1069 CTGGAG
BsoBI 106 CTCGGG C^[CT]CG[GA]G
190 CCCGAG
BssSI 1213 CACGAG CACGAG(-5/-1)
553 CTCGTG
BstYI 215 AGATCC [GA]^GATC[CT]
443 AGATCC
CfoI 126 GCGC GCG^C
128 GCGC
Cfr13I 984 GGGCC G^G[ACGT]CC
1209 GGACC
EaeI 2 TGGCCA [CT]^GGCC[GA]
1163 TGGCCA
FauI 940 GCGGG CCCGC(4/6)
982 GCGGG
HgaI 112 GACGC GACGC(5/10)
221 GCGTC
HhaI 126 GCGC GCG^C
128 GCGC
HinP1I 126 GCGC G^CGC
128 GCGC
HphI 900 GGTGA GGTGA(8/7)
1077 GGTGA
MaeII 617 ACGT A^CGT
704 ACGT
MflI 215 AGATCC [GA]^GATC[CT]
443 AGATCC
MluNI 2 TGGCCA TGG^CCA
1163 TGGCCA
MscI 2 TGGCCA TGG^CCA
1163 TGGCCA
PvuII 563 CAGCTG CAG^CTG
595 CAGCTG
SapI 171 GCTCTTC GCTCTTC(1/4)
963 GCTCTTC
Sau96I 984 GGGCC G^G[ACGT]CC
1209 GGACC
TfiI 722 GAATC G^A[AT]TC
1098 GATTC
Tsp509I 391 AATT ^AATT
1128 AATT
XcmI 5 CCAAGGAAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
287 CCAAGACTCCCCTGG
XhoII 215 AGATCC [GA]^GATC[CT]
443 AGATCC
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AluI AspHI BanII
BbvI BcnI BmyI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
Sau3AI ScrFI SfaNI Tru9I TseI
Tsp45I TspRI