Restriction map for
pF1KSDA1581
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BfaI BfrI BglII
BlnI BpmI BsaAI BsaHI BsaI
BseAI BsiEI BsiWI BsmBI BspDI
BspEI BspLU11I BspMI BsrBI BsrFI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MroI
MspA1I MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1351 GTCTAC GT^[AC][GT]AC
AcsI 7 AAATTC [GA]^AATT[CT]
AflIII 1040 ACGTGT A^C[GA][CT]GT
Alw44I 1267 GTGCAC G^TGCAC
ApaLI 1267 GTGCAC G^TGCAC
ApoI 7 AAATTC [GA]^AATT[CT]
AvaI 788 CCCGGG C^[CT]CG[GA]G
BanI 51 GGTGCC G^G[CT][GA]CC
BbsI 1402 GAAGAC GAAGAC(2/6)
BglI 814 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 711 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 711 GCTGAGC GC^T[ACGT]AGC
BpuAI 1402 GAAGAC GAAGAC(2/6)
BsaBI 1059 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 242 GAGAC GTCTC(1/5)
BsoBI 788 CCCGGG C^[CT]CG[GA]G
Bsp1407I 590 TGTACA T^GTACA
BspHI 173 TCATGA T^CATGA
BsrGI 590 TGTACA T^GTACA
BstYI 1115 GGATCT [GA]^GATC[CT]
CelII 711 GCTGAGC GC^T[ACGT]AGC
FauI 1303 GCGGG CCCGC(4/6)
HaeII 15 GGCGCT [GA]GCGC^[CT]
HgaI 424 GACGC GACGC(5/10)
MaeII 1040 ACGT A^CGT
MamI 1059 GATAGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1115 GGATCT [GA]^GATC[CT]
MseI 457 TTAA T^TAA
MslI 1068 CATCGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1325 CCATGG C^CATGG
PstI 809 CTGCAG CTGCA^G
RcaI 173 TCATGA T^CATGA
SfcI 809 CTGCAG C^T[GA][CT]AG
SmaI 788 CCCGGG CCC^GGG
SspBI 590 TGTACA T^GTACA
StuI 95 AGGCCT AGG^CCT
Tru9I 457 TTAA T^TAA
XhoII 1115 GGATCT [GA]^GATC[CT]
XmaCI 788 CCCGGG C^CCGGG
XmaI 788 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 177 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1185 GAAAACCTTC
AspHI 939 GAGCAC G[AT]GC[AT]^C
1267 GTGCAC
BalI 146 TGGCCA TGG^CCA
743 TGGCCA
BanII 562 GAGCCC G[GA]GC[CT]^C
1290 GGGCTC
BciVI 328 GGATAC GTATCC(6/5)
897 GTATCC
BsaWI 41 TCCGGT [AT]^CCGG[AT]
1355 ACCGGA
BseRI 1435 GAGGAG GAGGAG(10/8)
1465 GAGGAG
BsgI 349 CTGCAC GTGCAG(16/14)
612 CTGCAC
BsiHKAI 939 GAGCAC G[AT]GC[AT]^C
1267 GTGCAC
BsmFI 791 GGGAC GGGAC(10/14)
191 GTCCC
BsmI 553 GAATGC GAATGC(1/-1)
449 GCATTC
BsrDI 111 CATTGC GCAATG(2/0)
427 GCAATG
BssSI 942 CACGAG CACGAG(-5/-1)
1243 CTCGTG
BstXI 865 CCATACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1238 CCAGCCTCGTGG
DsaI 1325 CCATGG C^C[GA][CT]GG
1391 CCACGG
EcoT14I 795 CCTTGG C^C[AT][AT]GG
1325 CCATGG
HincII 772 GTCAAC GT[CT]^[GA]AC
876 GTTGAC
HindII 772 GTCAAC GT[CT]^[GA]AC
876 GTTGAC
MluNI 146 TGGCCA TGG^CCA
743 TGGCCA
MscI 146 TGGCCA TGG^CCA
743 TGGCCA
NspI 860 ACATGC [GA]CATG^[CT]
1421 ACATGC
PpuMI 189 GGGTCCC [GA]G^G[AT]CC[CT]
791 GGGACCT
StyI 795 CCTTGG C^C[AT][AT]GG
1325 CCATGG
TfiI 755 GAATC G^A[AT]TC
1318 GAATC
Tsp509I 8 AATT ^AATT
738 AATT
XmnI 177 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1185 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BmyI BsaJI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII EaeI EcoO109I
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TseI
Tsp45I TspRI TthHB8I XcmI