Restriction map for
pF1KSDA1587
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# NO CUT ENZYMES ( 97 enzymes) #
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AccIII AclI AflII AflIII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AspI BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BpuAI BsaAI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I ClaI DraI
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoRV EcoT22I FbaI FseI
HincII HindII HpaI KasI MamI
MfeI MluI MroI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SwaI Tth111I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 130 GACGTC GACGT^C
AccI 2479 GTAGAC GT^[AC][GT]AC
AcyI 130 GACGTC G[GA]^CG[CT]C
Alw44I 372 GTGCAC G^TGCAC
Aor51HI 2642 AGCGCT AGC^GCT
ApaLI 372 GTGCAC G^TGCAC
AviII 57 TGCGCA TGC^GCA
AvrII 1306 CCTAGG C^CTAGG
BglI 1792 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1306 CCTAGG C^CTAGG
BlpI 1486 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1486 GCTGAGC GC^T[ACGT]AGC
BsaHI 130 GACGTC G[GA]^CG[CT]C
BsaI 103 GGTCTC GGTCTC(1/5)
BsaWI 1268 ACCGGA [AT]^CCGG[AT]
Bsp1407I 2441 TGTACA T^GTACA
BsrBI 637 CCGCTC CCGCTC(-3/-3)
BsrDI 2806 GCAATG GCAATG(2/0)
BsrFI 780 GCCGGC [GA]^CCGG[CT]
BsrGI 2441 TGTACA T^GTACA
BstAPI 1497 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1311 GGTAACC G^GT[ACGT]ACC
BstPI 1311 GGTAACC G^GT[ACGT]ACC
CelII 1486 GCTGAGC GC^T[ACGT]AGC
Cfr10I 780 GCCGGC [GA]^CCGG[CT]
CpoI 1194 CGGACCG CG^G[AT]CCG
DraIII 608 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1717 GAAGAG CTCTTC(1/4)
Eco47III 2642 AGCGCT AGC^GCT
EcoO65I 1311 GGTAACC G^GT[ACGT]ACC
FspI 57 TGCGCA TGC^GCA
HgaI 384 GACGC GACGC(5/10)
Hin1I 130 GACGTC G[GA]^CG[CT]C
HindIII 2526 AAGCTT A^AGCTT
Ksp632I 1717 GAAGAG CTCTTC(1/4)
KspI 861 CCGCGG CCGC^GG
NaeI 780 GCCGGC GCC^GGC
NgoMIV 780 GCCGGC G^CCGGC
NspI 2613 GCATGT [GA]CATG^[CT]
PshAI 1877 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 69 CAGCTG CAG^CTG
RsrII 1194 CGGACCG CG^G[AT]CCG
SacII 861 CCGCGG CCGC^GG
SexAI 609 ACCTGGT A^CC[AT]GGT
SmaI 1436 CCCGGG CCC^GGG
SpeI 2403 ACTAGT A^CTAGT
SspBI 2441 TGTACA T^GTACA
SspI 1770 AATATT AAT^ATT
Tsp45I 382 GTGAC ^GT[GC]AC
XmaCI 1436 CCCGGG C^CCGGG
XmaI 1436 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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Acc65I 474 GGTACC G^GTACC
2160 GGTACC
AocI 1348 CCTAAGG CC^T[ACGT]AGG
1657 CCTGAGG
Asp718I 474 GGTACC G^GTACC
2160 GGTACC
AvaI 1233 CCCGAG C^[CT]CG[GA]G
1436 CCCGGG
BbvCI 217 GCTGAGG CCTCAGC(-5/-2)
2098 GCTGAGG
BseRI 1012 GAGGAG GAGGAG(10/8)
2470 GAGGAG
BsoBI 1233 CCCGAG C^[CT]CG[GA]G
1436 CCCGGG
BspMI 2623 ACCTGC ACCTGC(4/8)
2517 GCAGGT
BstF5I 2165 CATCC GGATG(2/0)
1826 GGATG
Bsu36I 1348 CCTAAGG CC^T[ACGT]AGG
1657 CCTGAGG
Eco81I 1348 CCTAAGG CC^T[ACGT]AGG
1657 CCTGAGG
FokI 2165 CATCC GGATG(9/13)
1826 GGATG
HaeII 265 AGCGCC [GA]GCGC^[CT]
2642 AGCGCT
KpnI 474 GGTACC GGTAC^C
2160 GGTACC
MaeII 131 ACGT A^CGT
1481 ACGT
MseI 1565 TTAA T^TAA
1681 TTAA
SmlI 261 CTCAAG C^T[CT][GA]AG
2634 CTTGAG
TaqI 946 TCGA T^CGA
1376 TCGA
Tru9I 1565 TTAA T^TAA
1681 TTAA
TthHB8I 946 TCGA T^CGA
1376 TCGA
XcmI 1316 CCAAGGCCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1409 CCATTAGTATTATGG
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TfiI
TseI Tsp509I TspRI XhoII