Restriction map for
pF1KSDA1609
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BamHI BbsI
BbvCI BcgI BciVI BfaI BfrI
BglII BlnI BpuAI BsaBI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MluI MroI MseI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspI StuI
SwaI TfiI Tru9I Tth111I XbaI
XcmI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 217 GTCGAC GT^[AC][GT]AC
AccII 1328 CGCG CG^CG
AcyI 797 GGCGCC G[GA]^CG[CT]C
Asp700I 166 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 797 GGCGCC GGCGC^C
BclI 72 TGATCA T^GATCA
BglI 549 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1309 CTGGAG CTGGAG(16/14)
BsaHI 797 GGCGCC G[GA]^CG[CT]C
BsaI 1235 GAGACC GGTCTC(1/5)
BsgI 484 GTGCAG GTGCAG(16/14)
BsmBI 668 CGTCTC CGTCTC(1/5)
BsmI 1323 GCATTC GAATGC(1/-1)
BspHI 323 TCATGA T^CATGA
BspLU11I 1034 ACATGT A^CATGT
BspMI 486 GCAGGT ACCTGC(4/8)
BstEII 186 GGTCACC G^GT[ACGT]ACC
BstPI 186 GGTCACC G^GT[ACGT]ACC
BstUI 1328 CGCG CG^CG
BstXI 840 CCAGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 393 GGATCT [GA]^GATC[CT]
EcoO65I 186 GGTCACC G^GT[ACGT]ACC
FauI 1176 CCCGC CCCGC(4/6)
FbaI 72 TGATCA T^GATCA
HaeII 797 GGCGCC [GA]GCGC^[CT]
HgaI 699 GCGTC GACGC(5/10)
Hin1I 797 GGCGCC G[GA]^CG[CT]C
HincII 217 GTCGAC GT[CT]^[GA]AC
HindII 217 GTCGAC GT[CT]^[GA]AC
HinfI 318 GAGTC G^A[ACGT]TC
KasI 797 GGCGCC G^GCGCC
MfeI 76 CAATTG C^AATTG
MflI 393 GGATCT [GA]^GATC[CT]
MunI 76 CAATTG C^AATTG
MvnI 1328 CGCG CG^CG
NarI 797 GGCGCC GG^CGCC
PaeR7I 886 CTCGAG C^TCGAG
PflMI 552 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 318 GAGTC GAGTC(4/5)
RcaI 323 TCATGA T^CATGA
SacI 817 GAGCTC GAGCT^C
SalI 217 GTCGAC G^TCGAC
SapI 169 GCTCTTC GCTCTTC(1/4)
SfcI 148 CTACAG C^T[GA][CT]AG
SfoI 797 GGCGCC GGC^GCC
SmaI 479 CCCGGG CCC^GGG
SmlI 886 CTCGAG C^T[CT][GA]AG
SphI 206 GCATGC GCATG^C
Van91I 552 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 886 CTCGAG C^TCGAG
XhoII 393 GGATCT [GA]^GATC[CT]
XmaCI 479 CCCGGG C^CCGGG
XmaI 479 CCCGGG C^CCGGG
XmnI 166 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AflIII 254 ACGTGT A^C[GA][CT]GT
1034 ACATGT
Alw44I 412 GTGCAC G^TGCAC
1158 GTGCAC
AlwI 1062 GATCC GGATC(4/5)
393 GGATC
AocI 52 CCTGAGG CC^T[ACGT]AGG
1291 CCTGAGG
ApaLI 412 GTGCAC G^TGCAC
1158 GTGCAC
BanI 788 GGCACC G^G[CT][GA]CC
797 GGCGCC
BbrPI 415 CACGTG CAC^GTG
1155 CACGTG
BlpI 420 GCTAAGC GC^T[ACGT]AGC
499 GCTCAGC
Bpu1102I 420 GCTAAGC GC^T[ACGT]AGC
499 GCTCAGC
BseRI 310 GAGGAG GAGGAG(10/8)
1246 GAGGAG
Bsp1407I 755 TGTACA T^GTACA
1004 TGTACA
BsrGI 755 TGTACA T^GTACA
1004 TGTACA
Bsu36I 52 CCTGAGG CC^T[ACGT]AGG
1291 CCTGAGG
CelII 420 GCTAAGC GC^T[ACGT]AGC
499 GCTCAGC
CfoI 798 GCGC GCG^C
1329 GCGC
DsaI 822 CCATGG C^C[GA][CT]GG
1047 CCATGG
EarI 170 CTCTTC CTCTTC(1/4)
315 GAAGAG
Eco81I 52 CCTGAGG CC^T[ACGT]AGG
1291 CCTGAGG
HhaI 798 GCGC GCG^C
1329 GCGC
HinP1I 798 GCGC G^CGC
1329 GCGC
Ksp632I 170 CTCTTC CTCTTC(1/4)
315 GAAGAG
MaeIII 187 GTCAC ^GT[ACGT]AC
572 GTGAC
MslI 324 CATGATTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CATGGCTGTG
NcoI 822 CCATGG C^CATGG
1047 CCATGG
PmaCI 415 CACGTG CAC^GTG
1155 CACGTG
PmlI 415 CACGTG CAC^GTG
1155 CACGTG
SspBI 755 TGTACA T^GTACA
1004 TGTACA
Tsp45I 187 GTCAC ^GT[GC]AC
572 GTGAC
Tsp509I 77 AATT ^AATT
1096 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BmyI BsaAI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MaeII MboI MboII
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I