Restriction map for
pF1KSDA1614
##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
Acc65I AccI AccIII AcsI AflII
Alw44I ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AviII BbsI
BclI BfrI BpuAI BsaBI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRI EcoRV EcoT22I FbaI FspI
HincII HindII HindIII HpaI KpnI
MamI MfeI MluI MroI MseI
MslI MunI NdeI NheI NotI
NruI NsiI NspV PacI PmeI
PshBI PvuI RcaI RsrII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3117 GACGTC GACGT^C
AclI 925 AACGTT AA^CGTT
AflIII 2904 ACATGT A^C[GA][CT]GT
AgeI 3233 ACCGGT A^CCGGT
AhdI 1062 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1062 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2883 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2032 CCTAGG C^CTAGG
BamHI 1778 GGATCC G^GATCC
BbrPI 1345 CACGTG CAC^GTG
BcgI 1619 GCAGCTGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2522 GTATCC GTATCC(6/5)
BfaI 2033 CTAG C^TAG
BglII 756 AGATCT A^GATCT
BlnI 2032 CCTAGG C^CTAGG
BsaAI 1345 CACGTG [CT]AC^GT[GA]
BsaI 836 GAGACC GGTCTC(1/5)
BspLU11I 2904 ACATGT A^CATGT
BsrBI 1396 GAGCGG CCGCTC(-3/-3)
BstEII 2500 GGTGACC G^GT[ACGT]ACC
BstPI 2500 GGTGACC G^GT[ACGT]ACC
Eam1105I 1062 GACTGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2500 GGTGACC G^GT[ACGT]ACC
FseI 3287 GGCCGGCC GGCCGG^CC
MaeI 2033 CTAG C^TAG
NspI 2904 ACATGT [GA]CATG^[CT]
PaeR7I 1961 CTCGAG C^TCGAG
PflFI 2883 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 209 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3233 ACCGGT A^CCGGT
PmaCI 1345 CACGTG CAC^GTG
PmlI 1345 CACGTG CAC^GTG
PshAI 592 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 925 AACGTT AA^CGTT
SapI 3024 GCTCTTC GCTCTTC(1/4)
ScaI 533 AGTACT AGT^ACT
SexAI 3248 ACCTGGT A^CC[AT]GGT
SmaI 1226 CCCGGG CCC^GGG
SmlI 1961 CTCGAG C^T[CT][GA]AG
Tsp509I 2322 AATT ^AATT
Tth111I 2883 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 209 CCAGGGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1961 CTCGAG C^TCGAG
XmaCI 1226 CCCGGG C^CCGGG
XmaI 1226 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 166 CCTCAGG CC^T[ACGT]AGG
2599 CCTCAGG
Aor51HI 821 AGCGCT AGC^GCT
3410 AGCGCT
BalI 287 TGGCCA TGG^CCA
1209 TGGCCA
BglI 441 GCCTGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1202 GCCGAGATGGC
BsaWI 2730 ACCGGA [AT]^CCGG[AT]
3233 ACCGGT
BspMI 437 ACCTGC ACCTGC(4/8)
2027 ACCTGC
Bsu36I 166 CCTCAGG CC^T[ACGT]AGG
2599 CCTCAGG
DrdI 711 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3117 GACGTCACAGTC
EarI 3025 CTCTTC CTCTTC(1/4)
3485 CTCTTC
Eco47III 821 AGCGCT AGC^GCT
3410 AGCGCT
Eco81I 166 CCTCAGG CC^T[ACGT]AGG
2599 CCTCAGG
EcoNI 2173 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3366 CCTGGTGGAGG
Ksp632I 3025 CTCTTC CTCTTC(1/4)
3485 CTCTTC
KspI 32 CCGCGG CCGC^GG
1445 CCGCGG
MluNI 287 TGGCCA TGG^CCA
1209 TGGCCA
MscI 287 TGGCCA TGG^CCA
1209 TGGCCA
NaeI 1581 GCCGGC GCC^GGC
3288 GCCGGC
NcoI 639 CCATGG C^CATGG
3498 CCATGG
NgoMIV 1581 GCCGGC G^CCGGC
3288 GCCGGC
SacII 32 CCGCGG CCGC^GG
1445 CCGCGG
StuI 1610 AGGCCT AGG^CCT
1957 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbeI BbvCI
BbvI BcnI BlpI BmyI BpmI
Bpu1102I BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MaeII MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XcmI XhoII