Restriction map for
pF1KSDA1617
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BamHI BcgI BciVI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiEI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRI
EcoT22I FbaI FseI FspI HpaI
KpnI MamI MluI MroI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AhdI 2236 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 399 GTGCAC G^TGCAC
ApaLI 399 GTGCAC G^TGCAC
AspEI 2236 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2349 GGCGCC GGCGC^C
BbrPI 294 CACGTG CAC^GTG
BbsI 2115 GTCTTC GAAGAC(2/6)
BfaI 1897 CTAG C^TAG
BglI 2515 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2009 AGATCT A^GATCT
BlpI 68 GCTCAGC GC^T[ACGT]AGC
BpmI 2449 CTGGAG CTGGAG(16/14)
Bpu1102I 68 GCTCAGC GC^T[ACGT]AGC
BpuAI 2115 GTCTTC GAAGAC(2/6)
BsiWI 1845 CGTACG C^GTACG
BsmBI 1792 GAGACG CGTCTC(1/5)
BsmI 206 GCATTC GAATGC(1/-1)
BspHI 1521 TCATGA T^CATGA
BsrBI 2292 GAGCGG CCGCTC(-3/-3)
BstEII 2475 GGTCACC G^GT[ACGT]ACC
BstPI 2475 GGTCACC G^GT[ACGT]ACC
CelII 68 GCTCAGC GC^T[ACGT]AGC
DraIII 385 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2236 GACACCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 2475 GGTCACC G^GT[ACGT]ACC
EcoRV 1932 GATATC GAT^ATC
HaeII 2349 GGCGCC [GA]GCGC^[CT]
HincII 1564 GTCAAC GT[CT]^[GA]AC
HindII 1564 GTCAAC GT[CT]^[GA]AC
KasI 2349 GGCGCC G^GCGCC
MaeI 1897 CTAG C^TAG
MfeI 1486 CAATTG C^AATTG
MunI 1486 CAATTG C^AATTG
NarI 2349 GGCGCC GG^CGCC
PmaCI 294 CACGTG CAC^GTG
PmlI 294 CACGTG CAC^GTG
PpuMI 522 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 1333 CTGCAG CTGCA^G
RcaI 1521 TCATGA T^CATGA
SacI 2215 GAGCTC GAGCT^C
SapI 1590 GCTCTTC GCTCTTC(1/4)
ScaI 605 AGTACT AGT^ACT
SfoI 2349 GGCGCC GGC^GCC
SspI 143 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 32 enzymes) #
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AcyI 2173 GACGCC G[GA]^CG[CT]C
2349 GGCGCC
AflIII 192 ACACGT A^C[GA][CT]GT
266 ACACGT
AlwI 1768 GATCC GGATC(4/5)
870 GGATC
AlwNI 304 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1499 CAGTGCCTG
AspI 364 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1858 GACCAAGTC
AvaI 2142 CTCGGG C^[CT]CG[GA]G
2401 CCCGAG
BbvCI 1585 CCTCAGC CCTCAGC(-5/-2)
2363 CCTCAGC
BsaHI 2173 GACGCC G[GA]^CG[CT]C
2349 GGCGCC
BsaI 86 GAGACC GGTCTC(1/5)
2203 GAGACC
BsmFI 1665 GGGAC GGGAC(10/14)
2407 GGGAC
BsoBI 2142 CTCGGG C^[CT]CG[GA]G
2401 CCCGAG
BstAPI 163 GCAAGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
303 GCAGCTGCTGC
BstF5I 908 GGATG GGATG(2/0)
2223 GGATG
BstYI 1767 AGATCC [GA]^GATC[CT]
2009 AGATCT
EcoNI 526 CCTCTGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1111 CCTCCCAAAGG
FokI 908 GGATG GGATG(9/13)
2223 GGATG
Hin1I 2173 GACGCC G[GA]^CG[CT]C
2349 GGCGCC
HindIII 111 AAGCTT A^AGCTT
913 AAGCTT
KspI 2135 CCGCGG CCGC^GG
2343 CCGCGG
MflI 1767 AGATCC [GA]^GATC[CT]
2009 AGATCT
MslI 2091 CATTTTCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2478 CACCGACGTG
NaeI 2312 GCCGGC GCC^GGC
2352 GCCGGC
NcoI 1373 CCATGG C^CATGG
2177 CCATGG
NgoMIV 2312 GCCGGC G^CCGGC
2352 GCCGGC
PflFI 364 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1858 GACCAAGTC
PvuII 304 CAGCTG CAG^CTG
1756 CAGCTG
SacII 2135 CCGCGG CCGC^GG
2343 CCGCGG
SfaNI 423 GATGC GCATC(5/9)
832 GATGC
SfcI 545 CTATAG C^T[GA][CT]AG
1333 CTGCAG
SmlI 108 CTCAAG C^T[CT][GA]AG
916 CTTGAG
Tth111I 364 GACGTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1858 GACCAAGTC
XhoII 1767 AGATCC [GA]^GATC[CT]
2009 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaII BalI BanI
BanII BbvI BcnI BmyI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrFI BsrI
BssKI BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I