Restriction map for
pF1KSDA1665
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BanI BbeI
BbrPI BbsI BbvCI BciVI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiWI BsmI BsoBI BspDI
BspHI BspLU11I BsrBI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MseI MspA1I
MunI MvnI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tru9I Tsp509I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccIII 32 TCCGGA T^CCGGA
AflIII 476 ACACGT A^C[GA][CT]GT
Alw44I 289 GTGCAC G^TGCAC
ApaI 490 GGGCCC GGGCC^C
ApaLI 289 GTGCAC G^TGCAC
AspI 25 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 602 GGACC G^G[AT]CC
BalI 101 TGGCCA TGG^CCA
BanII 490 GGGCCC G[GA]GC[CT]^C
BcgI 69 CGACTCTATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 450 CCGGG CC^[GC]GG
BlpI 497 GCTCAGC GC^T[ACGT]AGC
BpmI 160 CTGGAG CTGGAG(16/14)
Bpu1102I 497 GCTCAGC GC^T[ACGT]AGC
BsaWI 32 TCCGGA [AT]^CCGG[AT]
BseAI 32 TCCGGA T^CCGGA
Bsp1407I 116 TGTACA T^GTACA
BspEI 32 TCCGGA T^CCGGA
BspMI 372 GCAGGT ACCTGC(4/8)
BsrFI 430 ACCGGC [GA]^CCGG[CT]
BsrGI 116 TGTACA T^GTACA
Cac8I 348 GCCAGC GC[ACGT]^[ACGT]GC
CelII 497 GCTCAGC GC^T[ACGT]AGC
CfoI 191 GCGC GCG^C
Cfr10I 430 ACCGGC [GA]^CCGG[CT]
DdeI 498 CTCAG C^T[ACGT]AG
DraII 489 AGGGCCC [GA]G^G[ACGT]CC[CT]
EaeI 101 TGGCCA [CT]^GGCC[GA]
EcoO109I 489 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 148 GATATC GAT^ATC
EcoT14I 43 CCTTGG C^C[AT][AT]GG
HhaI 191 GCGC GCG^C
HinP1I 191 GCGC G^CGC
HincII 472 GTTGAC GT[CT]^[GA]AC
HindII 472 GTTGAC GT[CT]^[GA]AC
HphI 152 TCACC GGTGA(8/7)
MaeIII 336 GTCAC ^GT[ACGT]AC
MluNI 101 TGGCCA TGG^CCA
MroI 32 TCCGGA T^CCGGA
MscI 101 TGGCCA TGG^CCA
MslI 552 CACGCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 450 CCGGG CC^[GC]GG
NlaIII 423 CATG CATG^
PflFI 25 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
SgrAI 429 CACCGGCG C[GA]^CCGG[CT]G
SspBI 116 TGTACA T^GTACA
StuI 579 AGGCCT AGG^CCT
StyI 43 CCTTGG C^C[AT][AT]GG
TfiI 249 GATTC G^A[AT]TC
Tsp45I 336 GTCAC ^GT[GC]AC
Tth111I 25 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 444 CCGC CCGC(-3/-1)
367 GCGG
AspHI 289 GTGCAC G[AT]GC[AT]^C
407 GAGCAC
BamHI 35 GGATCC G^GATCC
562 GGATCC
BfaI 241 CTAG C^TAG
301 CTAG
BsiHKAI 289 GTGCAC G[AT]GC[AT]^C
407 GAGCAC
BsmAI 394 GAGAC GTCTC(1/5)
295 GTCTC
BsmBI 294 CGTCTC CGTCTC(1/5)
394 GAGACG
BsmFI 126 GGGAC GGGAC(10/14)
480 GTCCC
BsrI 159 ACTGG ACTGG(1/-1)
518 CCAGT
MaeI 241 CTAG C^TAG
301 CTAG
MboII 261 GAAGA GAAGA(8/7)
308 TCTTC
PstI 273 CTGCAG CTGCA^G
340 CTGCAG
SfcI 273 CTGCAG C^T[GA][CT]AG
340 CTGCAG
TaqI 311 TCGA T^CGA
359 TCGA
TthHB8I 311 TCGA T^CGA
359 TCGA
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AfaI AluI AlwI BbvI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI BstYI Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MaeII
MboI MflI MnlI MspI MvaI
NdeII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TseI TspRI
XhoII