Restriction map for
pF1KSDA1686
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII Aor51HI AscI
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BsaHI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstUI BstZ17I CelII CfoI ClaI
CpoI DraI Eco47III EcoNI EcoRV
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I KasI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflFI PmaCI
PmeI PmlI PshAI Psp1406I RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SmaI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AflIII 2284 ACGTGT A^C[GA][CT]GT
AgeI 504 ACCGGT A^CCGGT
AhdI 1777 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1777 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2300 CTCGAG C^[CT]CG[GA]G
BglI 419 GCCCTGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1152 AGATCT A^GATCT
BpmI 19 CTGGAG CTGGAG(16/14)
BsaAI 2283 TACGTG [CT]AC^GT[GA]
BsaBI 1251 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmAI 1878 GAGAC GTCTC(1/5)
BsmBI 1878 GAGACG CGTCTC(1/5)
BsoBI 2300 CTCGAG C^[CT]CG[GA]G
BspMI 247 ACCTGC ACCTGC(4/8)
BsrBI 1460 GAGCGG CCGCTC(-3/-3)
BssSI 437 CACGAG CACGAG(-5/-1)
BstEII 141 GGTCACC G^GT[ACGT]ACC
BstPI 141 GGTCACC G^GT[ACGT]ACC
DraIII 1494 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1803 GACTTTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 69 CGGCCG C^GGCCG
Eam1105I 1777 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 69 CGGCCG C^GGCCG
Eco52I 69 CGGCCG C^GGCCG
EcoO65I 141 GGTCACC G^GT[ACGT]ACC
EcoT22I 2209 ATGCAT ATGCA^T
FauI 1355 CCCGC CCCGC(4/6)
HgaI 1524 GCGTC GACGC(5/10)
HpaI 2610 GTTAAC GTT^AAC
MamI 1251 GATTAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 285 CAATTG C^AATTG
MunI 285 CAATTG C^AATTG
NcoI 1586 CCATGG C^CATGG
NdeI 3182 CATATG CA^TATG
NsiI 2209 ATGCAT ATGCA^T
PaeR7I 2300 CTCGAG C^TCGAG
PinAI 504 ACCGGT A^CCGGT
PpuMI 1452 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1416 CTGCAG CTGCA^G
PvuI 1203 CGATCG CGAT^CG
SspI 1594 AATATT AAT^ATT
StuI 1402 AGGCCT AGG^CCT
XbaI 3406 TCTAGA T^CTAGA
XhoI 2300 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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Alw44I 1090 GTGCAC G^TGCAC
2438 GTGCAC
AlwNI 812 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
2657 CAGAACCTG
ApaI 1183 GGGCCC GGGCC^C
1745 GGGCCC
ApaLI 1090 GTGCAC G^TGCAC
2438 GTGCAC
AseI 688 ATTAAT AT^TAAT
1021 ATTAAT
AsnI 688 ATTAAT AT^TAAT
1021 ATTAAT
Asp700I 1220 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3106 GAAATTGTTC
BsaWI 145 ACCGGA [AT]^CCGG[AT]
504 ACCGGT
BsiEI 69 CGGCCG CG[GA][CT]^CG
1203 CGATCG
BspHI 1275 TCATGA T^CATGA
1437 TCATGA
BstAPI 1072 GCATGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2175 GCAAGCTTTGC
BstXI 1580 CCAAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2000 CCATATATTTGG
DsaI 1586 CCATGG C^C[GA][CT]GG
1638 CCACGG
EaeI 69 CGGCCG [CT]^GGCC[GA]
349 CGGCCA
HincII 2610 GTTAAC GT[CT]^[GA]AC
3355 GTTGAC
HindII 2610 GTTAAC GT[CT]^[GA]AC
3355 GTTGAC
PflMI 1344 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3135 CCAAAATGTGG
PshBI 688 ATTAAT AT^TAAT
1021 ATTAAT
RcaI 1275 TCATGA T^CATGA
1437 TCATGA
SapI 2881 GAAGAGC GCTCTTC(1/4)
3322 GAAGAGC
SgrAI 126 CACCGGCG C[GA]^CCGG[CT]G
151 CACCGGCG
Van91I 1344 CCAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3135 CCAAAATGTGG
XmnI 1220 GAACAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3106 GAAATTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AspHI AvaII BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr10I Cfr13I
DdeI DpnII DraII EarI Eco81I
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII