Restriction map for
pF1KSDA1688
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# NO CUT ENZYMES ( 71 enzymes) #
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AatII AclI AcsI AflII AocI
ApoI AscI AseI AsnI Asp700I
AvrII BamHI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaBI
BsaI BsiWI BspDI BspHI BspLU11I
Bst1107I BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI Eco81I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI MamI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SfiI SfuI SnaBI SpeI
SspI SwaI Tru9I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 2906 GGTACC G^GTACC
AccIII 1333 TCCGGA T^CCGGA
AflIII 227 ACACGT A^C[GA][CT]GT
AgeI 142 ACCGGT A^CCGGT
AhdI 1438 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2998 GTGCAC G^TGCAC
ApaLI 2998 GTGCAC G^TGCAC
Asp718I 2906 GGTACC G^GTACC
AspEI 1438 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2500 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2054 TGCGCA TGC^GCA
BbvCI 1305 GCTGAGG CCTCAGC(-5/-2)
BcgI 2814 CGAGGTGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1873 CTAG C^TAG
BseAI 1333 TCCGGA T^CCGGA
BsmI 2820 GAATGC GAATGC(1/-1)
Bsp1407I 2255 TGTACA T^GTACA
BspEI 1333 TCCGGA T^CCGGA
BsrDI 686 GCAATG GCAATG(2/0)
BsrGI 2255 TGTACA T^GTACA
BssSI 2938 CACGAG CACGAG(-5/-1)
DraIII 267 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1438 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 2054 TGCGCA TGC^GCA
HincII 40 GTCGAC GT[CT]^[GA]AC
HindII 40 GTCGAC GT[CT]^[GA]AC
KpnI 2906 GGTACC GGTAC^C
MaeI 1873 CTAG C^TAG
MroI 1333 TCCGGA T^CCGGA
PflFI 2500 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 142 ACCGGT A^CCGGT
SalI 40 GTCGAC G^TCGAC
SbfI 3051 CCTGCAGG CCTGCA^GG
ScaI 2546 AGTACT AGT^ACT
SexAI 134 ACCTGGT A^CC[AT]GGT
SgrAI 141 CACCGGTG C[GA]^CCGG[CT]G
SmlI 535 CTTGAG C^T[CT][GA]AG
Sse8387I 3051 CCTGCAGG CCTGCA^GG
SspBI 2255 TGTACA T^GTACA
TfiI 60 GATTC G^A[AT]TC
Tsp509I 998 AATT ^AATT
Tth111I 2500 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 407 CCAGCTCCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccI 40 GTCGAC GT^[AC][GT]AC
1195 GTCTAC
Aor51HI 1139 AGCGCT AGC^GCT
2702 AGCGCT
ApaI 1086 GGGCCC GGGCC^C
1533 GGGCCC
BbrPI 2212 CACGTG CAC^GTG
2293 CACGTG
BsaWI 142 ACCGGT [AT]^CCGG[AT]
1333 TCCGGA
BsmBI 1905 CGTCTC CGTCTC(1/5)
2071 GAGACG
BssHII 348 GCGCGC G^CGCGC
1230 GCGCGC
BstYI 1886 AGATCC [GA]^GATC[CT]
2621 AGATCC
DrdI 2872 GACCCCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3154 GACCCGCGCGTC
Eco47III 1139 AGCGCT AGC^GCT
2702 AGCGCT
MflI 1886 AGATCC [GA]^GATC[CT]
2621 AGATCC
PmaCI 2212 CACGTG CAC^GTG
2293 CACGTG
PmlI 2212 CACGTG CAC^GTG
2293 CACGTG
StuI 2225 AGGCCT AGG^CCT
2862 AGGCCT
XhoII 1886 AGATCC [GA]^GATC[CT]
2621 AGATCC
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# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
KspI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
SacII SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SphI
StyI TaqI TseI Tsp45I TspRI
TthHB8I Van91I XmaCI XmaI