Restriction map for
pF1KSDA1701
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AvaI AvaII AviII BamHI BbeI
BbrPI BbsI BcgI BciVI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraII DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI HhaI HinP1I HpaI KasI
KpnI KspI MluI MroI MslI
MvnI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SspI SwaI TaqI TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AatII 887 GACGTC GACGT^C
AcsI 970 GAATTT [GA]^AATT[CT]
AcyI 887 GACGTC G[GA]^CG[CT]C
AfaI 317 GTAC GT^AC
AflII 994 CTTAAG C^TTAAG
AlwNI 488 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1361 CCTCAGG CC^T[ACGT]AGG
ApoI 970 GAATTT [GA]^AATT[CT]
AseI 613 ATTAAT AT^TAAT
AsnI 613 ATTAAT AT^TAAT
AspHI 218 GAGCTC G[AT]GC[AT]^C
AspI 141 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 481 CCTAGG C^CTAGG
BcnI 1258 CCGGG CC^[GC]GG
BfrI 994 CTTAAG C^TTAAG
BlnI 481 CCTAGG C^CTAGG
BsaBI 695 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 887 GACGTC G[GA]^CG[CT]C
BsiHKAI 218 GAGCTC G[AT]GC[AT]^C
BsmBI 205 GAGACG CGTCTC(1/5)
BsmI 949 GAATGC GAATGC(1/-1)
BspMI 456 ACCTGC ACCTGC(4/8)
BsrDI 1039 GCAATG GCAATG(2/0)
BstAPI 1030 GCACGGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 700 AGATCC [GA]^GATC[CT]
Bsu36I 1361 CCTCAGG CC^T[ACGT]AGG
DraI 1404 TTTAAA TTT^AAA
DsaI 251 CCATGG C^C[GA][CT]GG
Eco81I 1361 CCTCAGG CC^T[ACGT]AGG
EcoNI 481 CCTAGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 1258 CCGG C^CGG
Hin1I 887 GACGTC G[GA]^CG[CT]C
HincII 999 GTCAAC GT[CT]^[GA]AC
HindII 999 GTCAAC GT[CT]^[GA]AC
HindIII 1113 AAGCTT A^AGCTT
HpaII 1258 CCGG C^CGG
MaeII 888 ACGT A^CGT
MamI 695 GATTTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1562 CAATTG C^AATTG
MflI 700 AGATCC [GA]^GATC[CT]
MspI 1258 CCGG C^CGG
MunI 1562 CAATTG C^AATTG
NciI 1258 CCGGG CC^[GC]GG
NcoI 251 CCATGG C^CATGG
NdeI 1219 CATATG CA^TATG
PflFI 141 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 632 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 613 ATTAAT AT^TAAT
PstI 1427 CTGCAG CTGCA^G
PvuII 683 CAGCTG CAG^CTG
RsaI 317 GTAC GT^AC
SacI 218 GAGCTC GAGCT^C
SpeI 550 ACTAGT A^CTAGT
StuI 103 AGGCCT AGG^CCT
Tsp45I 1099 GTGAC ^GT[GC]AC
TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 141 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 632 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 700 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 298 ACATGT A^C[GA][CT]GT
1209 ACATGT
BalI 1520 TGGCCA TGG^CCA
1601 TGGCCA
BanI 427 GGTGCC G^G[CT][GA]CC
708 GGCACC
BanII 26 GAGCCC G[GA]GC[CT]^C
218 GAGCTC
BclI 1442 TGATCA T^GATCA
1575 TGATCA
BpmI 680 CTCCAG CTGGAG(16/14)
71 CTGGAG
BsmAI 205 GAGAC GTCTC(1/5)
1436 GAGAC
BsmFI 7 GGGAC GGGAC(10/14)
1260 GGGAC
BspLU11I 298 ACATGT A^CATGT
1209 ACATGT
BstF5I 864 CATCC GGATG(2/0)
1182 GGATG
EaeI 1520 TGGCCA [CT]^GGCC[GA]
1601 TGGCCA
FbaI 1442 TGATCA T^GATCA
1575 TGATCA
FokI 864 CATCC GGATG(9/13)
1182 GGATG
HphI 1059 TCACC GGTGA(8/7)
1255 TCACC
MluNI 1520 TGGCCA TGG^CCA
1601 TGGCCA
MscI 1520 TGGCCA TGG^CCA
1601 TGGCCA
MspA1I 683 CAGCTG C[AC]G^C[GT]G
752 CCGCTG
NspI 298 ACATGT [GA]CATG^[CT]
1209 ACATGT
PleI 237 GACTC GAGTC(4/5)
283 GAGTC
SfcI 107 CTGTAG C^T[GA][CT]AG
1427 CTGCAG
SmlI 994 CTTAAG C^T[CT][GA]AG
1581 CTTGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BbvCI BbvI
BfaI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HinfI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp509I