Restriction map for
pF1KSDA1719
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# NO CUT ENZYMES ( 79 enzymes) #
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Acc65I AccI AcsI AflII AflIII
AgeI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AviII AvrII
BbeI BcgI BciVI BfrI BglII
BlnI BsaBI BsiWI Bsp1407I BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
Bsu36I CpoI DraI EarI Eco81I
EcoRI EcoRV EcoT22I FspI HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MseI MunI
NarI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI RsrII SacII SalI SapI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tsp509I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 442 GACGTC GACGT^C
AclI 2122 AACGTT AA^CGTT
AhdI 2635 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1348 GTGCAC G^TGCAC
Aor51HI 1979 AGCGCT AGC^GCT
ApaLI 1348 GTGCAC G^TGCAC
AspEI 2635 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BspDI 898 ATCGAT AT^CGAT
BspHI 2687 TCATGA T^CATGA
BsrDI 1539 CATTGC GCAATG(2/0)
BstEII 2823 GGTGACC G^GT[ACGT]ACC
BstPI 2823 GGTGACC G^GT[ACGT]ACC
ClaI 898 ATCGAT AT^CGAT
EagI 2140 CGGCCG C^GGCCG
Eam1105I 2635 GACCTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2140 CGGCCG C^GGCCG
Eco47III 1979 AGCGCT AGC^GCT
Eco52I 2140 CGGCCG C^GGCCG
EcoO65I 2823 GGTGACC G^GT[ACGT]ACC
FseI 2137 GGCCGGCC GGCCGG^CC
HaeII 1979 AGCGCT [GA]GCGC^[CT]
HincII 2971 GTCAAC GT[CT]^[GA]AC
HindII 2971 GTCAAC GT[CT]^[GA]AC
NcoI 1853 CCATGG C^CATGG
NheI 1311 GCTAGC G^CTAGC
NspI 2612 GCATGT [GA]CATG^[CT]
PaeR7I 2716 CTCGAG C^TCGAG
Psp1406I 2122 AACGTT AA^CGTT
PvuI 3097 CGATCG CGAT^CG
RcaI 2687 TCATGA T^CATGA
SacI 1299 GAGCTC GAGCT^C
SexAI 1892 ACCTGGT A^CC[AT]GGT
SfiI 1007 GGCCACTGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 2423 CCCGGG CCC^GGG
TfiI 611 GAATC G^A[AT]TC
XhoI 2716 CTCGAG C^TCGAG
XmaCI 2423 CCCGGG C^CCGGG
XmaI 2423 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AccII 364 CGCG CG^CG
1593 CGCG
AccIII 803 TCCGGA T^CCGGA
1910 TCCGGA
AfaI 2761 GTAC GT^AC
2984 GTAC
BamHI 302 GGATCC G^GATCC
1234 GGATCC
BbrPI 1669 CACGTG CAC^GTG
2092 CACGTG
BbsI 2632 GAAGAC GAAGAC(2/6)
1161 GTCTTC
BfaI 1312 CTAG C^TAG
2212 CTAG
BpuAI 2632 GAAGAC GAAGAC(2/6)
1161 GTCTTC
BsaWI 803 TCCGGA [AT]^CCGG[AT]
1910 TCCGGA
BseAI 803 TCCGGA T^CCGGA
1910 TCCGGA
BsmBI 444 CGTCTC CGTCTC(1/5)
2868 CGTCTC
BsmI 113 GCATTC GAATGC(1/-1)
2105 GCATTC
BspEI 803 TCCGGA T^CCGGA
1910 TCCGGA
BssSI 670 CACGAG CACGAG(-5/-1)
1456 CTCGTG
BstAPI 1341 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1869 GCAAATCCTGC
BstUI 364 CGCG CG^CG
1593 CGCG
DraIII 2818 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
2905 CACACTGTG
DrdI 759 GACGCCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2032 GACCCCATTGTC
MaeI 1312 CTAG C^TAG
2212 CTAG
MroI 803 TCCGGA T^CCGGA
1910 TCCGGA
MvnI 364 CGCG CG^CG
1593 CGCG
NaeI 2138 GCCGGC GCC^GGC
2322 GCCGGC
NgoMIV 2138 GCCGGC G^CCGGC
2322 GCCGGC
PflMI 1651 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2575 CCAGCCCCTGG
PmaCI 1669 CACGTG CAC^GTG
2092 CACGTG
PmlI 1669 CACGTG CAC^GTG
2092 CACGTG
PvuII 1051 CAGCTG CAG^CTG
2516 CAGCTG
RsaI 2761 GTAC GT^AC
2984 GTAC
SbfI 2166 CCTGCAGG CCTGCA^GG
2964 CCTGCAGG
Sse8387I 2166 CCTGCAGG CCTGCA^GG
2964 CCTGCAGG
Van91I 1651 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2575 CCAGCCCCTGG
XcmI 938 CCACCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2057 CCAAGCGTGGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BclI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PflFI PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TseI Tsp45I
TspRI Tth111I TthHB8I XhoII