Restriction map for
pF1KSDA1722
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII AvrII BamHI
BbrPI BlnI BsiWI BsmBI BsmI
BspDI BspLU11I BsrDI BssHII BssSI
BstBI BstEII BstPI ClaI CpoI
Eam1105I Eco47III EcoO65I EcoT22I FseI
FspI KpnI MfeI MluI MunI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SalI ScaI SfiI SfuI SgrAI
SnaBI SpeI SwaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 3557 TCCGGA T^CCGGA
ApaI 3550 GGGCCC GGGCC^C
AspI 919 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1835 TGGCCA TGG^CCA
BbeI 4336 GGCGCC GGCGC^C
BciVI 1221 GGATAC GTATCC(6/5)
BglI 2593 GCCTCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2795 AGATCT A^GATCT
BlpI 1528 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1528 GCTAAGC GC^T[ACGT]AGC
BsaAI 2609 TACGTG [CT]AC^GT[GA]
BsaBI 2335 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3557 TCCGGA T^CCGGA
BspEI 3557 TCCGGA T^CCGGA
BspHI 4169 TCATGA T^CATGA
BspMI 2942 ACCTGC ACCTGC(4/8)
Bst1107I 1414 GTATAC GTA^TAC
BstZ17I 1414 GTATAC GTA^TAC
CelII 1528 GCTAAGC GC^T[ACGT]AGC
DraII 3549 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 274 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1738 CGGCCG C^GGCCG
EclXI 1738 CGGCCG C^GGCCG
Eco52I 1738 CGGCCG C^GGCCG
EcoO109I 3549 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRV 2962 GATATC GAT^ATC
HindIII 370 AAGCTT A^AGCTT
HpaI 2107 GTTAAC GTT^AAC
KasI 4336 GGCGCC G^GCGCC
KspI 3637 CCGCGG CCGC^GG
MamI 2335 GATGTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1835 TGGCCA TGG^CCA
MroI 3557 TCCGGA T^CCGGA
MscI 1835 TGGCCA TGG^CCA
NaeI 2060 GCCGGC GCC^GGC
NarI 4336 GGCGCC GG^CGCC
NdeI 2854 CATATG CA^TATG
NgoMIV 2060 GCCGGC G^CCGGC
PflFI 919 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 4169 TCATGA T^CATGA
SacI 1850 GAGCTC GAGCT^C
SacII 3637 CCGCGG CCGC^GG
SapI 3963 GAAGAGC GCTCTTC(1/4)
SbfI 2943 CCTGCAGG CCTGCA^GG
SexAI 2297 ACCTGGT A^CC[AT]GGT
SfoI 4336 GGCGCC GGC^GCC
SmaI 2912 CCCGGG CCC^GGG
SphI 4062 GCATGC GCATG^C
Sse8387I 2943 CCTGCAGG CCTGCA^GG
Tth111I 919 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2912 CCCGGG C^CCGGG
XmaI 2912 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 925 GTCTAC GT^[AC][GT]AC
1414 GTATAC
AccII 3638 CGCG CG^CG
4253 CGCG
AlwNI 1396 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
4456 CAGCCACTG
AseI 2097 ATTAAT AT^TAAT
2236 ATTAAT
AsnI 2097 ATTAAT AT^TAAT
2236 ATTAAT
BcgI 2862 CGATAATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
116 GCAACCGCTTCG
BclI 303 TGATCA T^GATCA
3897 TGATCA
BsiEI 1738 CGGCCG CG[GA][CT]^CG
4044 CGACCG
Bsp1407I 1858 TGTACA T^GTACA
3470 TGTACA
BsrFI 2060 GCCGGC [GA]^CCGG[CT]
2546 GCCGGT
BsrGI 1858 TGTACA T^GTACA
3470 TGTACA
BstAPI 4053 GCAGAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4455 GCAGCCACTGC
BstUI 3638 CGCG CG^CG
4253 CGCG
BstXI 581 CCATAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3186 CCATAGGGATGG
Cfr10I 2060 GCCGGC [GA]^CCGG[CT]
2546 GCCGGT
DrdI 3601 GACAATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3988 GACCCCCTGGTC
EcoNI 4068 CCTTAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4134 CCTAAACAAGG
EcoRI 29 GAATTC G^AATTC
1123 GAATTC
FbaI 303 TGATCA T^GATCA
3897 TGATCA
HaeII 2671 GGCGCT [GA]GCGC^[CT]
4336 GGCGCC
HincII 2107 GTTAAC GT[CT]^[GA]AC
2162 GTCAAC
HindII 2107 GTTAAC GT[CT]^[GA]AC
2162 GTCAAC
MvnI 3638 CGCG CG^CG
4253 CGCG
NcoI 1159 CCATGG C^CATGG
3269 CCATGG
NheI 2173 GCTAGC G^CTAGC
2314 GCTAGC
PshBI 2097 ATTAAT AT^TAAT
2236 ATTAAT
SspBI 1858 TGTACA T^GTACA
3470 TGTACA
SspI 1493 AATATT AAT^ATT
3649 AATATT
XbaI 1062 TCTAGA T^CTAGA
3426 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflII
AluI AlwI AocI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EaeI EarI Eco81I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI