Restriction map for
pF1KSDA1723
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# NO CUT ENZYMES ( 76 enzymes) #
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AatII Acc65I AclI AgeI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII AvrII BamHI BbeI
BlnI BsiWI BsmI Bsp1407I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I RsrII
SacII SalI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SspBI
SspI StuI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 4180 GTAGAC GT^[AC][GT]AC
AccIII 3305 TCCGGA T^CCGGA
AcyI 1502 GGCGTC G[GA]^CG[CT]C
AocI 2550 CCTCAGG CC^T[ACGT]AGG
ApaI 2086 GGGCCC GGGCC^C
BbrPI 2734 CACGTG CAC^GTG
BciVI 633 GGATAC GTATCC(6/5)
BsaAI 2734 CACGTG [CT]AC^GT[GA]
BsaBI 4348 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1502 GGCGTC G[GA]^CG[CT]C
BseAI 3305 TCCGGA T^CCGGA
BsmBI 2592 GAGACG CGTCTC(1/5)
BspDI 1435 ATCGAT AT^CGAT
BspEI 3305 TCCGGA T^CCGGA
BspHI 3182 TCATGA T^CATGA
BssSI 1226 CACGAG CACGAG(-5/-1)
Bsu36I 2550 CCTCAGG CC^T[ACGT]AGG
ClaI 1435 ATCGAT AT^CGAT
Eco81I 2550 CCTCAGG CC^T[ACGT]AGG
EcoRI 3726 GAATTC G^AATTC
Hin1I 1502 GGCGTC G[GA]^CG[CT]C
MamI 4348 GATCACGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 670 CAATTG C^AATTG
MroI 3305 TCCGGA T^CCGGA
MunI 670 CAATTG C^AATTG
PaeR7I 4271 CTCGAG C^TCGAG
PmaCI 2734 CACGTG CAC^GTG
PmlI 2734 CACGTG CAC^GTG
PshAI 187 GACTGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4353 CGATCG CGAT^CG
RcaI 3182 TCATGA T^CATGA
SbfI 3546 CCTGCAGG CCTGCA^GG
SphI 944 GCATGC GCATG^C
Sse8387I 3546 CCTGCAGG CCTGCA^GG
XcmI 3806 CCACTCAAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4271 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AflII 594 CTTAAG C^TTAAG
4461 CTTAAG
AflIII 36 ACATGT A^C[GA][CT]GT
4445 ACATGT
AhdI 1246 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1989 GACGCGCAGTC
AspEI 1246 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1989 GACGCGCAGTC
BalI 974 TGGCCA TGG^CCA
2538 TGGCCA
BbsI 1239 GTCTTC GAAGAC(2/6)
1773 GTCTTC
BclI 1095 TGATCA T^GATCA
2075 TGATCA
BfrI 594 CTTAAG C^TTAAG
4461 CTTAAG
BpuAI 1239 GTCTTC GAAGAC(2/6)
1773 GTCTTC
BsiEI 2868 CGACCG CG[GA][CT]^CG
4353 CGATCG
BspLU11I 36 ACATGT A^CATGT
4445 ACATGT
BstAPI 3288 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3603 GCAGACCCTGC
Eam1105I 1246 GACACTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1989 GACGCGCAGTC
EcoNI 1710 CCTCCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3731 CCTCTCCCAGG
FauI 2084 GCGGG CCCGC(4/6)
2310 GCGGG
FbaI 1095 TGATCA T^GATCA
2075 TGATCA
MluNI 974 TGGCCA TGG^CCA
2538 TGGCCA
MscI 974 TGGCCA TGG^CCA
2538 TGGCCA
NcoI 1061 CCATGG C^CATGG
2180 CCATGG
NdeI 415 CATATG CA^TATG
3823 CATATG
SacI 2031 GAGCTC GAGCT^C
3018 GAGCTC
SapI 2923 GAAGAGC GCTCTTC(1/4)
3333 GCTCTTC
SexAI 1682 ACCTGGT A^CC[AT]GGT
2977 ACCAGGT
SpeI 102 ACTAGT A^CTAGT
318 ACTAGT
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# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
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AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AspI AvaI AvaII BanI BanII
BbvCI BbvI BcgI BcnI BfaI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XhoII XmnI