Restriction map for
pF1KSDA1736
##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccII AclI AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I AviII BbeI BbrPI
BcgI BclI BfrI BsaAI BsaBI
BsiWI BspDI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
ClaI CpoI DraI DrdI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII KasI KspI MamI
MluI MvnI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 3529 GGTACC G^GTACC
AccI 497 GTCGAC GT^[AC][GT]AC
AccIII 29 TCCGGA T^CCGGA
AcsI 1976 GAATTT [GA]^AATT[CT]
AcyI 708 GACGCC G[GA]^CG[CT]C
AflIII 2841 ACATGT A^C[GA][CT]GT
Aor51HI 3604 AGCGCT AGC^GCT
ApoI 1976 GAATTT [GA]^AATT[CT]
Asp718I 3529 GGTACC G^GTACC
AspI 979 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3691 CCTAGG C^CTAGG
BciVI 32 GGATAC GTATCC(6/5)
BlnI 3691 CCTAGG C^CTAGG
BsaHI 708 GACGCC G[GA]^CG[CT]C
BsaWI 29 TCCGGA [AT]^CCGG[AT]
BseAI 29 TCCGGA T^CCGGA
BsiEI 1099 CGGCCG CG[GA][CT]^CG
BspEI 29 TCCGGA T^CCGGA
BspHI 2283 TCATGA T^CATGA
BspLU11I 2841 ACATGT A^CATGT
BspMI 1800 GCAGGT ACCTGC(4/8)
BstAPI 932 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 2737 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1099 CGGCCG C^GGCCG
EclXI 1099 CGGCCG C^GGCCG
Eco47III 3604 AGCGCT AGC^GCT
Eco52I 1099 CGGCCG C^GGCCG
Hin1I 708 GACGCC G[GA]^CG[CT]C
KpnI 3529 GGTACC GGTAC^C
MfeI 593 CAATTG C^AATTG
MroI 29 TCCGGA T^CCGGA
MunI 593 CAATTG C^AATTG
NaeI 1135 GCCGGC GCC^GGC
NgoMIV 1135 GCCGGC G^CCGGC
PflFI 979 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 490 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2283 TCATGA T^CATGA
SalI 497 GTCGAC G^TCGAC
SapI 749 GCTCTTC GCTCTTC(1/4)
Tth111I 979 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AhdI 872 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2225 GACGCTTTGTC
AocI 2038 CCTGAGG CC^T[ACGT]AGG
3206 CCTCAGG
AspEI 872 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2225 GACGCTTTGTC
BbvCI 823 CCTCAGC CCTCAGC(-5/-2)
3607 GCTGAGG
BglI 421 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2552 GCCAGAGGGGC
BglII 1996 AGATCT A^GATCT
2483 AGATCT
BsaI 2782 GAGACC GGTCTC(1/5)
2912 GAGACC
BsmBI 1509 GAGACG CGTCTC(1/5)
2980 GAGACG
Bsp1407I 1029 TGTACA T^GTACA
1385 TGTACA
BsrGI 1029 TGTACA T^GTACA
1385 TGTACA
BstXI 906 CCAGCACCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1886 CCAGCAGGCTGG
Bsu36I 2038 CCTGAGG CC^T[ACGT]AGG
3206 CCTCAGG
Eam1105I 872 GACAGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2225 GACGCTTTGTC
Eco81I 2038 CCTGAGG CC^T[ACGT]AGG
3206 CCTCAGG
HaeII 839 AGCGCC [GA]GCGC^[CT]
3604 AGCGCT
HpaI 598 GTTAAC GTT^AAC
2323 GTTAAC
MaeII 2289 ACGT A^CGT
2807 ACGT
NspI 734 ACATGC [GA]CATG^[CT]
2841 ACATGT
PaeR7I 950 CTCGAG C^TCGAG
1855 CTCGAG
SexAI 2915 ACCTGGT A^CC[AT]GGT
3411 ACCTGGT
SmlI 950 CTCGAG C^T[CT][GA]AG
1855 CTCGAG
SspBI 1029 TGTACA T^GTACA
1385 TGTACA
XhoI 950 CTCGAG C^TCGAG
1855 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrBI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII