Restriction map for
pF1KSDA1753
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
AccIII AclI AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI AspEI AspI AviII BamHI
BbrPI BciVI BclI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspHI BspLU11I BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SfuI SgrAI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I Van91I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 259 GACGTC GACGT^C
Acc65I 356 GGTACC G^GTACC
AflII 2308 CTTAAG C^TTAAG
AflIII 101 ACACGT A^C[GA][CT]GT
Asp718I 356 GGTACC G^GTACC
AvrII 1125 CCTAGG C^CTAGG
BalI 522 TGGCCA TGG^CCA
BfrI 2308 CTTAAG C^TTAAG
BglII 875 AGATCT A^GATCT
BlnI 1125 CCTAGG C^CTAGG
BsaI 800 GAGACC GGTCTC(1/5)
BsiEI 50 CGGCCG CG[GA][CT]^CG
BsmI 1491 GAATGC GAATGC(1/-1)
Bsp1407I 1031 TGTACA T^GTACA
BsrGI 1031 TGTACA T^GTACA
BssSI 394 CACGAG CACGAG(-5/-1)
BstXI 555 CCAGTCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 50 CGGCCG C^GGCCG
EarI 1419 CTCTTC CTCTTC(1/4)
EclXI 50 CGGCCG C^GGCCG
Eco52I 50 CGGCCG C^GGCCG
EcoNI 597 CCTCAGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 115 GAATTC G^AATTC
HindIII 1884 AAGCTT A^AGCTT
KpnI 356 GGTACC GGTAC^C
Ksp632I 1419 CTCTTC CTCTTC(1/4)
KspI 673 CCGCGG CCGC^GG
MluNI 522 TGGCCA TGG^CCA
MscI 522 TGGCCA TGG^CCA
MslI 784 CACGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 503 CCATGG C^CATGG
SacII 673 CCGCGG CCGC^GG
ScaI 836 AGTACT AGT^ACT
SexAI 1265 ACCAGGT A^CC[AT]GGT
SfiI 501 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 1083 GCATGC GCATG^C
SspBI 1031 TGTACA T^GTACA
TfiI 383 GAATC G^A[AT]TC
Tsp45I 974 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 262 GTCTAC GT^[AC][GT]AC
478 GTCTAC
AccII 674 CGCG CG^CG
2163 CGCG
AcsI 115 GAATTC [GA]^AATT[CT]
1290 AAATTT
AlwI 594 GATCC GGATC(4/5)
1828 GGATC
ApoI 115 GAATTC [GA]^AATT[CT]
1290 AAATTT
AspHI 1665 GAGCTC G[AT]GC[AT]^C
2365 GAGCTC
BcgI 31 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1637 GCATCACCATCG
BsaAI 102 CACGTA [CT]AC^GT[GA]
952 CACGTA
BsiHKAI 1665 GAGCTC G[AT]GC[AT]^C
2365 GAGCTC
BsmBI 761 CGTCTC CGTCTC(1/5)
824 GAGACG
BsrFI 215 GCCGGT [GA]^CCGG[CT]
725 ACCGGC
BstUI 674 CGCG CG^CG
2163 CGCG
Cfr10I 215 GCCGGT [GA]^CCGG[CT]
725 ACCGGC
DraIII 193 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
888 CACCAAGTG
HgaI 826 GACGC GACGC(5/10)
1478 GCGTC
MaeIII 368 GTTAC ^GT[ACGT]AC
974 GTGAC
MseI 1312 TTAA T^TAA
2309 TTAA
MvnI 674 CGCG CG^CG
2163 CGCG
PpuMI 930 AGGACCC [GA]G^G[AT]CC[CT]
1020 GGGTCCT
PvuII 918 CAGCTG CAG^CTG
995 CAGCTG
SacI 1665 GAGCTC GAGCT^C
2365 GAGCTC
SmlI 1410 CTCAAG C^T[CT][GA]AG
2308 CTTAAG
StuI 291 AGGCCT AGG^CCT
509 AGGCCT
Tru9I 1312 TTAA T^TAA
2309 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwNI
ApaI Asp700I AvaI AvaII BanI
BanII BbeI BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
StyI TaqI TseI Tsp509I TspRI
TthHB8I XhoII XmaCI XmaI XmnI