Restriction map for
pF1KSDA1778
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AgeI AlwI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BamHI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DrdI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 723 GACGTC GACGT^C
AflIII 19 ACACGT A^C[GA][CT]GT
AhdI 844 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 436 GTGCAC G^TGCAC
Aor51HI 791 AGCGCT AGC^GCT
ApaI 573 GGGCCC GGGCC^C
ApaLI 436 GTGCAC G^TGCAC
AspEI 844 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 731 TGGCCA TGG^CCA
BbrPI 20 CACGTG CAC^GTG
BbsI 961 GTCTTC GAAGAC(2/6)
BfaI 545 CTAG C^TAG
BglI 420 GCCCTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 935 AGATCT A^GATCT
BpuAI 961 GTCTTC GAAGAC(2/6)
BseRI 63 CTCCTC GAGGAG(10/8)
BsiEI 777 CGACCG CG[GA][CT]^CG
BsrBI 88 CCGCTC CCGCTC(-3/-3)
BsrI 594 CCAGT ACTGG(1/-1)
BssHII 700 GCGCGC G^CGCGC
BstAPI 297 GCAGCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 273 CATCC GGATG(2/0)
BstYI 935 AGATCT [GA]^GATC[CT]
DpnII 936 GATC ^GATC
DraII 770 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 868 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 731 TGGCCA [CT]^GGCC[GA]
Eam1105I 844 GACAAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 791 AGCGCT AGC^GCT
EcoNI 828 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 770 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 734 CCAAGG C^C[AT][AT]GG
FokI 273 CATCC GGATG(9/13)
MaeI 545 CTAG C^TAG
MboI 936 GATC ^GATC
MflI 935 AGATCT [GA]^GATC[CT]
MluNI 731 TGGCCA TGG^CCA
MscI 731 TGGCCA TGG^CCA
MslI 150 CACGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 936 GATC ^GATC
NheI 544 GCTAGC G^CTAGC
NspI 185 GCATGT [GA]CATG^[CT]
PmaCI 20 CACGTG CAC^GTG
PmlI 20 CACGTG CAC^GTG
PpuMI 770 GGGACCC [GA]G^G[AT]CC[CT]
SacI 370 GAGCTC GAGCT^C
SapI 90 GCTCTTC GCTCTTC(1/4)
Sau3AI 936 GATC ^GATC
ScaI 353 AGTACT AGT^ACT
SfaNI 704 GCATC GCATC(5/9)
SmlI 814 CTCAAG C^T[CT][GA]AG
StuI 836 AGGCCT AGG^CCT
StyI 734 CCAAGG C^C[AT][AT]GG
TspRI 57 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 530 CCACGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 935 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AlwNI 208 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
595 CAGTCGCTG
AspHI 370 GAGCTC G[AT]GC[AT]^C
436 GTGCAC
BpmI 535 CTGGAG CTGGAG(16/14)
829 CTGGAG
BsaAI 20 CACGTG [CT]AC^GT[GA]
133 TACGTG
BsgI 147 CTGCAC GTGCAG(16/14)
879 GTGCAG
BsiHKAI 370 GAGCTC G[AT]GC[AT]^C
436 GTGCAC
BspMI 284 ACCTGC ACCTGC(4/8)
895 ACCTGC
BsrFI 332 GCCGGC [GA]^CCGG[CT]
611 GCCGGC
BssSI 301 CTCGTG CACGAG(-5/-1)
373 CTCGTG
Cfr10I 332 GCCGGC [GA]^CCGG[CT]
611 GCCGGC
EarI 91 CTCTTC CTCTTC(1/4)
262 CTCTTC
HgaI 667 GACGC GACGC(5/10)
679 GACGC
HinfI 169 GACTC G^A[ACGT]TC
856 GAGTC
Ksp632I 91 CTCTTC CTCTTC(1/4)
262 CTCTTC
KspI 77 CCGCGG CCGC^GG
642 CCGCGG
MaeIII 136 GTGAC ^GT[ACGT]AC
458 GTGAC
NaeI 332 GCCGGC GCC^GGC
611 GCCGGC
NgoMIV 332 GCCGGC G^CCGGC
611 GCCGGC
NlaIII 186 CATG CATG^
870 CATG
PflMI 594 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
867 CCACATGGTGG
PleI 169 GACTC GAGTC(4/5)
856 GAGTC
PstI 337 CTGCAG CTGCA^G
966 CTGCAG
SacII 77 CCGCGG CCGC^GG
642 CCGCGG
SfcI 337 CTGCAG C^T[GA][CT]AG
966 CTGCAG
SmaI 218 CCCGGG CCC^GGG
408 CCCGGG
TaqI 359 TCGA T^CGA
842 TCGA
Tsp45I 136 GTGAC ^GT[GC]AC
458 GTGAC
TthHB8I 359 TCGA T^CGA
842 TCGA
Van91I 594 CCAGTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
867 CCACATGGTGG
XmaCI 218 CCCGGG C^CCGGG
408 CCCGGG
XmaI 218 CCCGGG C^CCGGG
408 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbeI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DsaI EcoRII FauI Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI KasI
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIV RsaI Sau96I ScrFI SfoI
TseI