################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## AatII AclI AhdI Alw44I ApaLI AseI AsnI AspEI BbrPI BsaBI BsiWI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I HpaI MamI MfeI MluI MunI NdeI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII ScaI SfuI SnaBI SpeI SspBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 20 enzymes) # ################################### AflII 7435 CTTAAG C^TTAAG AgeI 2288 ACCGGT A^CCGGT AscI 94 GGCGCGCC GG^CGCGCC AspI 7105 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1939 CCTAGG C^CTAGG BfrI 7435 CTTAAG C^TTAAG BglII 5525 AGATCT A^GATCT BlnI 1939 CCTAGG C^CTAGG BsaAI 8821 TACGTG [CT]AC^GT[GA] BspLU11I 3915 ACATGT A^CATGT BssHII 95 GCGCGC G^CGCGC DraI 3663 TTTAAA TTT^AAA EcoRV 5541 GATATC GAT^ATC EcoT22I 2421 ATGCAT ATGCA^T NheI 8814 GCTAGC G^CTAGC NsiI 2421 ATGCAT ATGCA^T PflFI 7105 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2288 ACCGGT A^CCGGT SgrAI 8326 CGCCGGCG C[GA]^CCGG[CT]G Tth111I 7105 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 4204 GTCGAC GT^[AC][GT]AC 8214 GTCGAC AcsI 7834 GAATTC [GA]^AATT[CT] 8512 GAATTC AflIII 446 ACACGT A^C[GA][CT]GT 3915 ACATGT ApoI 7834 GAATTC [GA]^AATT[CT] 8512 GAATTC AviII 6392 TGCGCA TGC^GCA 7466 TGCGCA BciVI 3494 GTATCC GTATCC(6/5) 7016 GTATCC BclI 1596 TGATCA T^GATCA 5312 TGATCA BsrDI 9087 CATTGC GCAATG(2/0) 5713 GCAATG DrdI 3510 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 6835 GACCAGGGGGTC EcoRI 7834 GAATTC G^AATTC 8512 GAATTC FbaI 1596 TGATCA T^GATCA 5312 TGATCA FseI 50 GGCCGGCC GGCCGG^CC 818 GGCCGGCC FspI 6392 TGCGCA TGC^GCA 7466 TGCGCA HindIII 3889 AAGCTT A^AGCTT 6515 AAGCTT MseI 3664 TTAA T^TAA 7436 TTAA NruI 1031 TCGCGA TCG^CGA 3127 TCGCGA PaeR7I 723 CTCGAG C^TCGAG 8168 CTCGAG PshAI 721 GACTCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1315 GACGAGGGTC SacI 4171 GAGCTC GAGCT^C 4834 GAGCTC SalI 4204 GTCGAC G^TCGAC 8214 GTCGAC SbfI 2793 CCTGCAGG CCTGCA^GG 2837 CCTGCAGG SphI 4271 GCATGC GCATG^C 8996 GCATGC Sse8387I 2793 CCTGCAGG CCTGCA^GG 2837 CCTGCAGG Tru9I 3664 TTAA T^TAA 7436 TTAA XhoI 723 CTCGAG C^TCGAG 8168 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 168 enzymes) # ######################################### Acc65I AccII AccIII AciI AcyI AfaI AluI AlwI AlwNI AocI Aor51HI ApaI Asp700I Asp718I AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvCI BbvI BcgI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BssSI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NotI NspI PflMI PleI PpuMI PstI PvuII RsaI SacII SapI Sau3AI Sau96I ScrFI SexAI SfaNI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI