Restriction map for
pF1KSDA1809
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI AocI Aor51HI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BbvCI BclI
BglII BlnI BsaAI BsaWI BseAI
BsiWI BsmBI BsmI BspDI BspEI
BsrBI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI DraI DraIII
Eam1105I Eco47III Eco81I EcoO65I EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KasI KpnI MfeI
MroI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
SalI SapI SbfI ScaI SfcI
SfiI SfoI SfuI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 1633 GACGTC GACGT^C
ApaI 2184 GGGCCC GGGCC^C
AscI 1563 GGCGCGCC GG^CGCGCC
BanI 1351 GGCACC G^G[CT][GA]CC
BcgI 1904 GCATCAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 635 GTATCC GTATCC(6/5)
BglI 1193 GCCACACGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 734 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1321 GGTCTC GGTCTC(1/5)
Bsp1407I 514 TGTACA T^GTACA
BspHI 126 TCATGA T^CATGA
BspLU11I 511 ACATGT A^CATGT
BsrDI 212 GCAATG GCAATG(2/0)
BsrGI 514 TGTACA T^GTACA
BssSI 1573 CACGAG CACGAG(-5/-1)
CpoI 1781 CGGACCG CG^G[AT]CCG
DrdI 1302 GACGGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1937 CGGCCG C^GGCCG
EclXI 1937 CGGCCG C^GGCCG
Eco52I 1937 CGGCCG C^GGCCG
EcoRI 46 GAATTC G^AATTC
FseI 1000 GGCCGGCC GGCCGG^CC
MamI 734 GATCAGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1235 ACGCGT A^CGCGT
PpuMI 1691 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1315 GACGACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 126 TCATGA T^CATGA
RsrII 1781 CGGACCG CG^G[AT]CCG
SgrAI 1183 CACCGGCG C[GA]^CCGG[CT]G
SspBI 514 TGTACA T^GTACA
StuI 1679 AGGCCT AGG^CCT
Tsp45I 313 GTCAC ^GT[GC]AC
XbaI 349 TCTAGA T^CTAGA
XcmI 1355 CCAGCGACTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 36 enzymes) #
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AcyI 1068 GACGCC G[GA]^CG[CT]C
1633 GACGTC
AflII 33 CTTAAG C^TTAAG
2311 CTTAAG
Alw44I 1180 GTGCAC G^TGCAC
1239 GTGCAC
AlwNI 219 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1690 CAGGTCCTG
ApaLI 1180 GTGCAC G^TGCAC
1239 GTGCAC
Asp700I 67 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1917 GAAGGAGTTC
BalI 866 TGGCCA TGG^CCA
1400 TGGCCA
BbsI 1272 GAAGAC GAAGAC(2/6)
1759 GAAGAC
BfrI 33 CTTAAG C^TTAAG
2311 CTTAAG
BpuAI 1272 GAAGAC GAAGAC(2/6)
1759 GAAGAC
BsaHI 1068 GACGCC G[GA]^CG[CT]C
1633 GACGTC
BsiEI 1595 CGGTCG CG[GA][CT]^CG
1937 CGGCCG
BspMI 716 ACCTGC ACCTGC(4/8)
1928 ACCTGC
BsrI 398 ACTGG ACTGG(1/-1)
539 CCAGT
BssHII 1382 GCGCGC G^CGCGC
1564 GCGCGC
BstYI 2010 AGATCC [GA]^GATC[CT]
2126 GGATCT
EcoNI 887 CCTTTGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1722 CCTGGCCGAGG
EcoT14I 681 CCATGG C^C[AT][AT]GG
935 CCATGG
Hin1I 1068 GACGCC G[GA]^CG[CT]C
1633 GACGTC
KspI 625 CCGCGG CCGC^GG
1812 CCGCGG
MaeIII 313 GTCAC ^GT[ACGT]AC
1023 GTTAC
MflI 2010 AGATCC [GA]^GATC[CT]
2126 GGATCT
MluNI 866 TGGCCA TGG^CCA
1400 TGGCCA
MscI 866 TGGCCA TGG^CCA
1400 TGGCCA
MslI 877 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2170 CATATTAGTG
NcoI 681 CCATGG C^CATGG
935 CCATGG
NspI 511 ACATGT [GA]CATG^[CT]
1280 ACATGC
PaeR7I 1912 CTCGAG C^TCGAG
2301 CTCGAG
PflMI 539 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1689 CCAGGTCCTGG
SacII 625 CCGCGG CCGC^GG
1812 CCGCGG
SexAI 1172 ACCTGGT A^CC[AT]GGT
1688 ACCAGGT
StyI 681 CCATGG C^C[AT][AT]GG
935 CCATGG
Van91I 539 CCAGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1689 CCAGGTCCTGG
XhoI 1912 CTCGAG C^TCGAG
2301 CTCGAG
XhoII 2010 AGATCC [GA]^GATC[CT]
2126 GGATCT
XmnI 67 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1917 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AspHI AvaI
AvaII BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SmaI SmlI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmaCI XmaI