Restriction map for
pF1KSDA1810
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccII AccIII
AcsI AflII AflIII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
AvrII BamHI BbrPI BcgI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
KpnI KspI MamI MfeI MflI
MluI MroI MseI MunI MvnI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfuI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI TfiI Tru9I
Tsp509I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AclI 236 AACGTT AA^CGTT
Alw44I 556 GTGCAC G^TGCAC
AlwI 894 GGATC GGATC(4/5)
AocI 1621 CCTGAGG CC^T[ACGT]AGG
ApaLI 556 GTGCAC G^TGCAC
BalI 596 TGGCCA TGG^CCA
BbeI 1658 GGCGCC GGCGC^C
BbsI 160 GTCTTC GAAGAC(2/6)
BciVI 672 GTATCC GTATCC(6/5)
BclI 548 TGATCA T^GATCA
BfaI 1523 CTAG C^TAG
BpuAI 160 GTCTTC GAAGAC(2/6)
BsrBI 1330 GAGCGG CCGCTC(-3/-3)
BsrDI 703 GCAATG GCAATG(2/0)
BsrFI 1655 GCCGGC [GA]^CCGG[CT]
BstXI 587 CCATGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1621 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1655 GCCGGC [GA]^CCGG[CT]
DraIII 430 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1621 CCTGAGG CC^T[ACGT]AGG
FbaI 548 TGATCA T^GATCA
HgaI 1387 GACGC GACGC(5/10)
HincII 1462 GTCAAC GT[CT]^[GA]AC
HindII 1462 GTCAAC GT[CT]^[GA]AC
KasI 1658 GGCGCC G^GCGCC
MaeI 1523 CTAG C^TAG
MaeII 237 ACGT A^CGT
MluNI 596 TGGCCA TGG^CCA
MscI 596 TGGCCA TGG^CCA
NaeI 1655 GCCGGC GCC^GGC
NarI 1658 GGCGCC GG^CGCC
NcoI 1007 CCATGG C^CATGG
NgoMIV 1655 GCCGGC G^CCGGC
Psp1406I 236 AACGTT AA^CGTT
SfiI 1439 GGCCCCCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1658 GGCGCC GGC^GCC
SgrAI 1654 CGCCGGCG C[GA]^CCGG[CT]G
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1387 GACGCC G[GA]^CG[CT]C
1658 GGCGCC
ApaI 1011 GGGCCC GGGCC^C
1438 GGGCCC
AspI 108 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
709 GACAATGTC
AvaII 363 GGACC G^G[AT]CC
438 GGACC
BbvCI 937 CCTCAGC CCTCAGC(-5/-2)
358 GCTGAGG
BsaHI 1387 GACGCC G[GA]^CG[CT]C
1658 GGCGCC
BsgI 427 CTGCAC GTGCAG(16/14)
1076 GTGCAG
BsmFI 461 GGGAC GGGAC(10/14)
1027 GTCCC
BspHI 536 TCATGA T^CATGA
545 TCATGA
BspMI 820 ACCTGC ACCTGC(4/8)
829 ACCTGC
BstAPI 719 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1356 GCAGAGCCTGC
DsaI 434 CCGTGG C^C[GA][CT]GG
1007 CCATGG
EaeI 26 CGGCCA [CT]^GGCC[GA]
596 TGGCCA
EcoT14I 1007 CCATGG C^C[AT][AT]GG
1433 CCTTGG
HaeII 36 GGCGCT [GA]GCGC^[CT]
1658 GGCGCC
Hin1I 1387 GACGCC G[GA]^CG[CT]C
1658 GGCGCC
HinfI 1597 GAGTC G^A[ACGT]TC
1678 GAGTC
NspI 479 ACATGC [GA]CATG^[CT]
986 GCATGT
PflFI 108 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
709 GACAATGTC
PleI 1597 GAGTC GAGTC(4/5)
1678 GAGTC
PvuII 786 CAGCTG CAG^CTG
1115 CAGCTG
RcaI 536 TCATGA T^CATGA
545 TCATGA
StuI 146 AGGCCT AGG^CCT
1582 AGGCCT
StyI 1007 CCATGG C^C[AT][AT]GG
1433 CCTTGG
Tth111I 108 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
709 GACAATGTC
XcmI 116 CCAGCCCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
827 CCACCTGCCGTATGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SspBI TseI Tsp45I
TspRI Van91I