Restriction map for
pF1KSDA1813
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
BamHI BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BpuAI BsaAI
BsaHI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeII MluI
MseI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Van91I
XbaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1481 GTCTAC GT^[AC][GT]AC
AccIII 284 TCCGGA T^CCGGA
Aor51HI 926 AGCGCT AGC^GCT
AspI 288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 2 TGGCCA TGG^CCA
BbvCI 1594 GCTGAGG CCTCAGC(-5/-2)
BglII 1346 AGATCT A^GATCT
BsaBI 420 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 284 TCCGGA T^CCGGA
BsiEI 1601 CGGCCG CG[GA][CT]^CG
BspEI 284 TCCGGA T^CCGGA
EagI 1601 CGGCCG C^GGCCG
EclXI 1601 CGGCCG C^GGCCG
Eco47III 926 AGCGCT AGC^GCT
Eco52I 1601 CGGCCG C^GGCCG
EcoNI 1113 CCTGCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 103 GGCCGGCC GGCCGG^CC
KspI 360 CCGCGG CCGC^GG
MamI 420 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1207 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MroI 284 TCCGGA T^CCGGA
MscI 2 TGGCCA TGG^CCA
MslI 1863 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1207 CAATTG C^AATTG
NaeI 104 GCCGGC GCC^GGC
NcoI 747 CCATGG C^CATGG
NgoMIV 104 GCCGGC G^CCGGC
PaeR7I 464 CTCGAG C^TCGAG
PflFI 288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 360 CCGCGG CCGC^GG
SfiI 1956 GGCCGAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 465 TCGA T^CGA
Tsp509I 1208 AATT ^AATT
Tth111I 288 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 465 TCGA T^CGA
XhoI 464 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 890 GTAC GT^AC
1872 GTAC
AvrII 235 CCTAGG C^CTAGG
852 CCTAGG
BanI 703 GGTGCC G^G[CT][GA]CC
734 GGCACC
BlnI 235 CCTAGG C^CTAGG
852 CCTAGG
BlpI 1558 GCTGAGC GC^T[ACGT]AGC
1911 GCTCAGC
Bpu1102I 1558 GCTGAGC GC^T[ACGT]AGC
1911 GCTCAGC
BsaI 1001 GAGACC GGTCTC(1/5)
1312 GAGACC
BsaWI 284 TCCGGA [AT]^CCGG[AT]
697 ACCGGA
BstXI 171 CCAGCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
747 CCATGGCTCTGG
BstYI 419 AGATCC [GA]^GATC[CT]
1346 AGATCT
CelII 1558 GCTGAGC GC^T[ACGT]AGC
1911 GCTCAGC
EarI 573 CTCTTC CTCTTC(1/4)
585 CTCTTC
HgaI 537 GACGC GACGC(5/10)
645 GACGC
Ksp632I 573 CTCTTC CTCTTC(1/4)
585 CTCTTC
MaeIII 253 GTCAC ^GT[ACGT]AC
294 GTCAC
MflI 419 AGATCC [GA]^GATC[CT]
1346 AGATCT
PpuMI 495 GGGTCCT [GA]G^G[AT]CC[CT]
788 GGGTCCC
RsaI 890 GTAC GT^AC
1872 GTAC
SmaI 115 CCCGGG CCC^GGG
1931 CCCGGG
SmlI 464 CTCGAG C^T[CT][GA]AG
1306 CTTGAG
Tsp45I 253 GTCAC ^GT[GC]AC
294 GTCAC
XcmI 141 CCACGGCCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
807 CCACTCAGACAGTGG
XhoII 419 AGATCC [GA]^GATC[CT]
1346 AGATCT
XmaCI 115 CCCGGG C^CCGGG
1931 CCCGGG
XmaI 115 CCCGGG C^CCGGG
1931 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI TspRI