################################## # NO CUT ENZYMES ( 105 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BbsI BciVI BclI BfrI BlnI BpuAI BsaBI BsaHI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRV EcoT22I FbaI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NarI NdeI NotI NruI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 1501 GTCTAC GT^[AC][GT]AC AflIII 1390 ACGTGT A^C[GA][CT]GT AlwI 1836 GATCC GGATC(4/5) AocI 1930 CCTGAGG CC^T[ACGT]AGG ApaI 2009 GGGCCC GGGCC^C AspI 1243 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1389 CACGTG CAC^GTG BcgI 2103 CGAGGCCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2135 AGATCT A^GATCT BlpI 2262 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2262 GCTGAGC GC^T[ACGT]AGC BsaAI 1389 CACGTG [CT]AC^GT[GA] BsaWI 1694 ACCGGA [AT]^CCGG[AT] BsmI 1319 GCATTC GAATGC(1/-1) BsrBI 967 GAGCGG CCGCTC(-3/-3) Bsu36I 1930 CCTGAGG CC^T[ACGT]AGG CelII 2262 GCTGAGC GC^T[ACGT]AGC DsaI 656 CCATGG C^C[GA][CT]GG EagI 137 CGGCCG C^GGCCG EclXI 137 CGGCCG C^GGCCG Eco52I 137 CGGCCG C^GGCCG Eco81I 1930 CCTGAGG CC^T[ACGT]AGG EcoNI 63 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 327 GAATTC G^AATTC FseI 1189 GGCCGGCC GGCCGG^CC HaeII 2321 AGCGCC [GA]GCGC^[CT] HgaI 1605 GACGC GACGC(5/10) MslI 2014 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 656 CCATGG C^CATGG NheI 2157 GCTAGC G^CTAGC PflFI 1243 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1389 CACGTG CAC^GTG PmlI 1389 CACGTG CAC^GTG PvuI 1354 CGATCG CGAT^CG SapI 34 GAAGAGC GCTCTTC(1/4) SbfI 1890 CCTGCAGG CCTGCA^GG SphI 1523 GCATGC GCATG^C Sse8387I 1890 CCTGCAGG CCTGCA^GG StuI 1934 AGGCCT AGG^CCT TfiI 282 GAATC G^A[AT]TC Tth111I 1243 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 313 CCAGAGACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcsI 327 GAATTC [GA]^AATT[CT] 438 AAATTT AfaI 1065 GTAC GT^AC 1692 GTAC ApoI 327 GAATTC [GA]^AATT[CT] 438 AAATTT BglI 1181 GCCGGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2002 GCCCTGCGGGC BseRI 1684 GAGGAG GAGGAG(10/8) 2389 GAGGAG BsiEI 137 CGGCCG CG[GA][CT]^CG 1354 CGATCG BsrFI 803 GCCGGC [GA]^CCGG[CT] 1190 GCCGGC BstYI 1835 AGATCC [GA]^GATC[CT] 2135 AGATCT Cfr10I 803 GCCGGC [GA]^CCGG[CT] 1190 GCCGGC MaeII 1390 ACGT A^CGT 1625 ACGT MflI 1835 AGATCC [GA]^GATC[CT] 2135 AGATCT NaeI 803 GCCGGC GCC^GGC 1190 GCCGGC NgoMIV 803 GCCGGC G^CCGGC 1190 GCCGGC PaeR7I 740 CTCGAG C^TCGAG 767 CTCGAG RsaI 1065 GTAC GT^AC 1692 GTAC XhoI 740 CTCGAG C^TCGAG 767 CTCGAG XhoII 1835 AGATCC [GA]^GATC[CT] 2135 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstXI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I