Restriction map for
pF1KSDA1831
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# NO CUT ENZYMES ( 96 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbrPI BciVI BclI BfrI
BglII BlnI BsaBI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
DraI DraIII EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HincII
HindII HpaI KpnI MamI MfeI
MluI MroI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RcaI SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Tsp509I Tth111I XbaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 695 GACGTC GACGT^C
AhdI 610 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 893 AGCGCT AGC^GCT
AspEI 610 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 731 TGCGCA TGC^GCA
BalI 1223 TGGCCA TGG^CCA
BamHI 1366 GGATCC G^GATCC
BlpI 672 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 672 GCTCAGC GC^T[ACGT]AGC
BsaAI 43 TACGTG [CT]AC^GT[GA]
BsaI 361 GAGACC GGTCTC(1/5)
BsaWI 763 ACCGGA [AT]^CCGG[AT]
BsmBI 1577 GAGACG CGTCTC(1/5)
BsmI 1858 GCATTC GAATGC(1/-1)
BspMI 1244 ACCTGC ACCTGC(4/8)
BsrBI 686 GAGCGG CCGCTC(-3/-3)
BssHII 858 GCGCGC G^CGCGC
BstAPI 1248 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1811 CCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1366 GGATCC [GA]^GATC[CT]
CelII 672 GCTCAGC GC^T[ACGT]AGC
CpoI 765 CGGACCG CG^G[AT]CCG
DrdI 1081 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 610 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 893 AGCGCT AGC^GCT
FspI 731 TGCGCA TGC^GCA
HindIII 1478 AAGCTT A^AGCTT
KspI 249 CCGCGG CCGC^GG
MaeIII 144 GTCAC ^GT[ACGT]AC
MflI 1366 GGATCC [GA]^GATC[CT]
MluNI 1223 TGGCCA TGG^CCA
MscI 1223 TGGCCA TGG^CCA
MseI 1067 TTAA T^TAA
NaeI 643 GCCGGC GCC^GGC
NgoMIV 643 GCCGGC G^CCGGC
PaeR7I 1728 CTCGAG C^TCGAG
PflMI 1475 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1453 GACCAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 765 CGGACCG CG^G[AT]CCG
SacI 1762 GAGCTC GAGCT^C
SacII 249 CCGCGG CCGC^GG
SmlI 1728 CTCGAG C^T[CT][GA]AG
StuI 1789 AGGCCT AGG^CCT
Tru9I 1067 TTAA T^TAA
Tsp45I 144 GTCAC ^GT[GC]AC
Van91I 1475 CCAAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1728 CTCGAG C^TCGAG
XhoII 1366 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 33 enzymes) #
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Alw44I 87 GTGCAC G^TGCAC
1012 GTGCAC
AlwI 1367 GATCC GGATC(4/5)
1366 GGATC
AlwNI 117 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1240 CAGGACCTG
AocI 1486 CCTGAGG CC^T[ACGT]AGG
1713 CCTGAGG
ApaLI 87 GTGCAC G^TGCAC
1012 GTGCAC
BbeI 100 GGCGCC GGCGC^C
1096 GGCGCC
BbsI 154 GTCTTC GAAGAC(2/6)
1622 GTCTTC
BcgI 1785 CGAGAGGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
872 GCAGCGGCTTCG
BfaI 1258 CTAG C^TAG
1525 CTAG
BglI 965 GCCCCGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1204 GCCGTGGAGGC
BpmI 1467 CTCCAG CTGGAG(16/14)
1445 CTGGAG
BpuAI 154 GTCTTC GAAGAC(2/6)
1622 GTCTTC
BsiEI 641 CGGCCG CG[GA][CT]^CG
797 CGGCCG
Bsu36I 1486 CCTGAGG CC^T[ACGT]AGG
1713 CCTGAGG
EagI 641 CGGCCG C^GGCCG
797 CGGCCG
EarI 1648 GAAGAG CTCTTC(1/4)
1759 GAAGAG
EclXI 641 CGGCCG C^GGCCG
797 CGGCCG
Eco52I 641 CGGCCG C^GGCCG
797 CGGCCG
Eco81I 1486 CCTGAGG CC^T[ACGT]AGG
1713 CCTGAGG
HphI 741 GGTGA GGTGA(8/7)
1880 TCACC
KasI 100 GGCGCC G^GCGCC
1096 GGCGCC
Ksp632I 1648 GAAGAG CTCTTC(1/4)
1759 GAAGAG
MaeI 1258 CTAG C^TAG
1525 CTAG
MslI 834 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1698 CATCGCCATG
NarI 100 GGCGCC GG^CGCC
1096 GGCGCC
PleI 1288 GACTC GAGTC(4/5)
142 GAGTC
PstI 1246 CTGCAG CTGCA^G
1602 CTGCAG
SapI 1648 GAAGAGC GCTCTTC(1/4)
1759 GAAGAGC
SfaNI 1557 GATGC GCATC(5/9)
1741 GATGC
SfcI 1246 CTGCAG C^T[GA][CT]AG
1602 CTGCAG
SfoI 100 GGCGCC GGC^GCC
1096 GGCGCC
TfiI 490 GATTC G^A[AT]TC
650 GATTC
XcmI 1697 CCATCGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1811 CCAACCCCCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
ApaI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BmyI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
RsaI Sau3AI Sau96I ScrFI SmaI
TaqI TseI TspRI TthHB8I XmaCI
XmaI