Restriction map for
pF1KSDA1835
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AgeI AocI ApoI AscI Asp718I
AviII BamHI BbrPI BcgI BciVI
BclI BglII BsaAI BsaBI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI DraI EagI EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI KpnI
MamI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI SalI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 1297 GACGTC GACGT^C
AccII 865 CGCG CG^CG
AflII 354 CTTAAG C^TTAAG
AhdI 498 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1552 GTGCAC G^TGCAC
AlwNI 1744 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 239 AGCGCT AGC^GCT
ApaLI 1552 GTGCAC G^TGCAC
AseI 805 ATTAAT AT^TAAT
AsnI 805 ATTAAT AT^TAAT
AspEI 498 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1579 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1396 CCCGAG C^[CT]CG[GA]G
AvrII 1344 CCTAGG C^CTAGG
BcnI 56 CCGGG CC^[GC]GG
BfrI 354 CTTAAG C^TTAAG
BglI 19 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1344 CCTAGG C^CTAGG
BsaWI 509 ACCGGA [AT]^CCGG[AT]
BsmBI 1299 CGTCTC CGTCTC(1/5)
BsmI 367 GCATTC GAATGC(1/-1)
BsoBI 1396 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1751 TGTACA T^GTACA
BspLU11I 257 ACATGT A^CATGT
BspMI 792 GCAGGT ACCTGC(4/8)
BsrGI 1751 TGTACA T^GTACA
BssSI 1549 CTCGTG CACGAG(-5/-1)
BstUI 865 CGCG CG^CG
CpoI 277 CGGACCG CG^G[AT]CCG
DraIII 1630 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1380 GACTGACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 498 GACCGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 239 AGCGCT AGC^GCT
HincII 700 GTCAAC GT[CT]^[GA]AC
HindII 700 GTCAAC GT[CT]^[GA]AC
HindIII 605 AAGCTT A^AGCTT
KspI 864 CCGCGG CCGC^GG
MslI 247 CACTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 865 CGCG CG^CG
NciI 56 CCGGG CC^[GC]GG
PflFI 1579 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 646 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 622 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 805 ATTAAT AT^TAAT
RsrII 277 CGGACCG CG^G[AT]CCG
SacII 864 CCGCGG CCGC^GG
SapI 1186 GAAGAGC GCTCTTC(1/4)
SbfI 429 CCTGCAGG CCTGCA^GG
Sse8387I 429 CCTGCAGG CCTGCA^GG
SspBI 1751 TGTACA T^GTACA
StuI 212 AGGCCT AGG^CCT
TaqI 395 TCGA T^CGA
Tsp509I 808 AATT ^AATT
Tth111I 1579 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 395 TCGA T^CGA
Van91I 646 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 1605 GTAC GT^AC
1752 GTAC
AflIII 257 ACATGT A^C[GA][CT]GT
1385 ACACGT
ApaI 373 GGGCCC GGGCC^C
1348 GGGCCC
Asp700I 1015 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1497 GAAGGCTTTC
BalI 761 TGGCCA TGG^CCA
1049 TGGCCA
BanI 586 GGCGCC G^G[CT][GA]CC
754 GGCGCC
BbeI 586 GGCGCC GGCGC^C
754 GGCGCC
BbvCI 1192 CCTCAGC CCTCAGC(-5/-2)
940 GCTGAGG
BfaI 307 CTAG C^TAG
1345 CTAG
BsaI 772 GAGACC GGTCTC(1/5)
1407 GGTCTC
BstAPI 103 GCAGAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1477 GCAGTAGATGC
BstYI 284 AGATCC [GA]^GATC[CT]
479 AGATCC
EcoNI 627 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
681 CCTGGCCCAGG
FauI 1727 CCCGC CCCGC(4/6)
714 GCGGG
HapII 56 CCGG C^CGG
510 CCGG
HpaII 56 CCGG C^CGG
510 CCGG
KasI 586 GGCGCC G^GCGCC
754 GGCGCC
MaeI 307 CTAG C^TAG
1345 CTAG
MaeII 1298 ACGT A^CGT
1387 ACGT
MflI 284 AGATCC [GA]^GATC[CT]
479 AGATCC
MluNI 761 TGGCCA TGG^CCA
1049 TGGCCA
MscI 761 TGGCCA TGG^CCA
1049 TGGCCA
MspI 56 CCGG C^CGG
510 CCGG
NarI 586 GGCGCC GG^CGCC
754 GGCGCC
NcoI 764 CCATGG C^CATGG
947 CCATGG
NspI 257 ACATGT [GA]CATG^[CT]
641 ACATGC
PleI 45 GACTC GAGTC(4/5)
317 GACTC
RsaI 1605 GTAC GT^AC
1752 GTAC
SacI 411 GAGCTC GAGCT^C
1356 GAGCTC
SfoI 586 GGCGCC GGC^GCC
754 GGCGCC
TfiI 1239 GATTC G^A[AT]TC
1711 GAATC
XcmI 41 CCAAGACTCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1697 CCAACAGCGCCCTGG
XhoII 284 AGATCC [GA]^GATC[CT]
479 AGATCC
XmnI 1015 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1497 GAAGGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AluI AlwI AspHI
AvaII BanII BbsI BbvI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HinfI HphI ItaI Ksp632I
MaeIII MboI MboII MnlI MseI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
Tru9I TseI Tsp45I TspRI