Restriction map for
pF1KSDA1848
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbeI BbvCI BcgI BciVI
BfrI BlnI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI HincII HindII HindIII
HpaI KasI KpnI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 1117 GTCTAC GT^[AC][GT]AC
AcsI 76 AAATTT [GA]^AATT[CT]
AfaI 288 GTAC GT^AC
AflIII 559 ACGTGT A^C[GA][CT]GT
AlwNI 356 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 76 AAATTT [GA]^AATT[CT]
Asp700I 118 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 431 TGCGCA TGC^GCA
BclI 344 TGATCA T^GATCA
BfaI 596 CTAG C^TAG
BglII 164 AGATCT A^GATCT
BlpI 1206 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1206 GCTCAGC GC^T[ACGT]AGC
BsgI 58 GTGCAG GTGCAG(16/14)
BsmBI 718 CGTCTC CGTCTC(1/5)
BspMI 758 ACCTGC ACCTGC(4/8)
BssSI 772 CACGAG CACGAG(-5/-1)
BstEII 261 GGTCACC G^GT[ACGT]ACC
BstF5I 507 GGATG GGATG(2/0)
BstPI 261 GGTCACC G^GT[ACGT]ACC
BstXI 305 CCAGTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 164 AGATCT [GA]^GATC[CT]
CelII 1206 GCTCAGC GC^T[ACGT]AGC
DrdI 241 GACAGGAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 380 CCGCGG C^C[GA][CT]GG
EarI 1009 GAAGAG CTCTTC(1/4)
EcoNI 1005 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 261 GGTCACC G^GT[ACGT]ACC
EcoT14I 536 CCAAGG C^C[AT][AT]GG
FbaI 344 TGATCA T^GATCA
FokI 507 GGATG GGATG(9/13)
FspI 431 TGCGCA TGC^GCA
HaeII 625 AGCGCC [GA]GCGC^[CT]
HinfI 734 GAATC G^A[ACGT]TC
Ksp632I 1009 GAAGAG CTCTTC(1/4)
KspI 380 CCGCGG CCGC^GG
MaeI 596 CTAG C^TAG
MflI 164 AGATCT [GA]^GATC[CT]
NspI 821 ACATGC [GA]CATG^[CT]
PvuII 841 CAGCTG CAG^CTG
RsaI 288 GTAC GT^AC
SacII 380 CCGCGG CCGC^GG
SfiI 969 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 992 AGGCCT AGG^CCT
StyI 536 CCAAGG C^C[AT][AT]GG
TaqI 466 TCGA T^CGA
TfiI 734 GAATC G^A[AT]TC
TthHB8I 466 TCGA T^CGA
XhoII 164 AGATCT [GA]^GATC[CT]
XmnI 118 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 24 GGCGTC G[GA]^CG[CT]C
494 GGCGTC
BalI 81 TGGCCA TGG^CCA
968 TGGCCA
BbrPI 558 CACGTG CAC^GTG
748 CACGTG
BpmI 442 CTGGAG CTGGAG(16/14)
727 CTGGAG
BsaAI 558 CACGTG [CT]AC^GT[GA]
748 CACGTG
BsaHI 24 GGCGTC G[GA]^CG[CT]C
494 GGCGTC
BsrFI 491 ACCGGC [GA]^CCGG[CT]
698 ACCGGC
Cfr10I 491 ACCGGC [GA]^CCGG[CT]
698 ACCGGC
DpnII 165 GATC ^GATC
345 GATC
Hin1I 24 GGCGTC G[GA]^CG[CT]C
494 GGCGTC
MaeII 559 ACGT A^CGT
749 ACGT
MboI 165 GATC ^GATC
345 GATC
MluNI 81 TGGCCA TGG^CCA
968 TGGCCA
MscI 81 TGGCCA TGG^CCA
968 TGGCCA
MslI 744 CACTCACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
876 CACCTTCGTG
MspA1I 380 CCGCGG C[AC]G^C[GT]G
841 CAGCTG
NdeII 165 GATC ^GATC
345 GATC
PflMI 146 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
819 CCACATGCTGG
PmaCI 558 CACGTG CAC^GTG
748 CACGTG
PmlI 558 CACGTG CAC^GTG
748 CACGTG
PstI 190 CTGCAG CTGCA^G
542 CTGCAG
SacI 670 GAGCTC GAGCT^C
1105 GAGCTC
Sau3AI 165 GATC ^GATC
345 GATC
SmaI 50 CCCGGG CCC^GGG
131 CCCGGG
SmlI 168 CTTGAG C^T[CT][GA]AG
255 CTCAAG
Tsp509I 77 AATT ^AATT
607 AATT
Van91I 146 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
819 CCACATGCTGG
XcmI 203 CCAGTGCCCGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
944 CCACTATGCCTGTGG
XmaCI 50 CCCGGG C^CCGGG
131 CCCGGG
XmaI 50 CCCGGG C^CCGGG
131 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BglI BmyI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeIII MboII MnlI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PpuMI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI