Restriction map for
pF1KSDA1852
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# NO CUT ENZYMES ( 129 enzymes) #
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Acc65I AccI AccII AccIII AciI
AclI AflII AflIII AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI AvaI
AviII AvrII BalI BamHI BbeI
BbvCI BcgI BcnI BfaI BfrI
BglII BlnI BsaBI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoRV FauI
FseI FspI HaeII HgaI HhaI
HinP1I HpaI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1218 GACGTC GACGT^C
AcyI 1218 GACGTC G[GA]^CG[CT]C
AhdI 279 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 322 CCTGAGG CC^T[ACGT]AGG
AspEI 279 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 31 GGTGCC G^G[CT][GA]CC
BbrPI 115 CACGTG CAC^GTG
BbsI 592 GTCTTC GAAGAC(2/6)
BciVI 102 GTATCC GTATCC(6/5)
BclI 915 TGATCA T^GATCA
BglI 79 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 592 GTCTTC GAAGAC(2/6)
BsaAI 115 CACGTG [CT]AC^GT[GA]
BsaHI 1218 GACGTC G[GA]^CG[CT]C
BsaI 277 GAGACC GGTCTC(1/5)
BsaWI 2093 ACCGGA [AT]^CCGG[AT]
BsmAI 277 GAGAC GTCTC(1/5)
BspMI 290 ACCTGC ACCTGC(4/8)
BsrDI 2085 CATTGC GCAATG(2/0)
BstYI 2059 AGATCC [GA]^GATC[CT]
Bsu36I 322 CCTGAGG CC^T[ACGT]AGG
Cac8I 372 GCAGGC GC[ACGT]^[ACGT]GC
DraI 1618 TTTAAA TTT^AAA
DraII 250 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 200 TGGCCG [CT]^GGCC[GA]
Eam1105I 279 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 322 CCTGAGG CC^T[ACGT]AGG
EcoNI 129 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 250 AGGACCC [GA]G^G[ACGT]CC[CT]
FbaI 915 TGATCA T^GATCA
HapII 2094 CCGG C^CGG
Hin1I 1218 GACGTC G[GA]^CG[CT]C
HincII 67 GTTGAC GT[CT]^[GA]AC
HindII 67 GTTGAC GT[CT]^[GA]AC
HindIII 1640 AAGCTT A^AGCTT
HpaII 2094 CCGG C^CGG
MflI 2059 AGATCC [GA]^GATC[CT]
MspI 2094 CCGG C^CGG
NdeI 1588 CATATG CA^TATG
PmaCI 115 CACGTG CAC^GTG
PmlI 115 CACGTG CAC^GTG
PpuMI 250 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1184 CTGCAG CTGCA^G
SapI 352 GAAGAGC GCTCTTC(1/4)
ScaI 609 AGTACT AGT^ACT
TfiI 1314 GAATC G^A[AT]TC
XcmI 73 CCATGGGCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 2059 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BlpI 1319 GCTCAGC GC^T[ACGT]AGC
1823 GCTCAGC
Bpu1102I 1319 GCTCAGC GC^T[ACGT]AGC
1823 GCTCAGC
BseRI 133 GAGGAG GAGGAG(10/8)
141 GAGGAG
BsmFI 1216 GGGAC GGGAC(10/14)
1649 GGGAC
Bsp1407I 1416 TGTACA T^GTACA
2172 TGTACA
BsrGI 1416 TGTACA T^GTACA
2172 TGTACA
BstEII 286 GGTCACC G^GT[ACGT]ACC
774 GGTTACC
BstPI 286 GGTCACC G^GT[ACGT]ACC
774 GGTTACC
CelII 1319 GCTCAGC GC^T[ACGT]AGC
1823 GCTCAGC
EcoO65I 286 GGTCACC G^GT[ACGT]ACC
774 GGTTACC
EcoT22I 1597 ATGCAT ATGCA^T
2269 ATGCAT
MwoI 79 GCCTCCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2288 GCTTTATATGC
NcoI 73 CCATGG C^CATGG
468 CCATGG
NsiI 1597 ATGCAT ATGCA^T
2269 ATGCAT
NspI 376 GCATGT [GA]CATG^[CT]
726 ACATGC
SfaNI 270 GATGC GCATC(5/9)
877 GATGC
SspBI 1416 TGTACA T^GTACA
2172 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BanII BbvI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
DsaI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MvaI NdeII NlaIII NlaIV PleI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfcI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XmnI