Restriction map for
pF1KSDA1857
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI AlwI AscI AseI
AsnI Asp718I AspEI AvrII BamHI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaBI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrGI BssSI Bst1107I
BstBI BstYI BstZ17I CelII ClaI
CpoI DpnII DraI DrdI Eam1105I
EcoNI EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MroI MseI MunI
NdeI NdeII NheI NotI NruI
NsiI NspV PacI PaeR7I PleI
PmeI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
Sau3AI SbfI SexAI SfiI SfuI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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AcsI 963 GAATTT [GA]^AATT[CT]
AgeI 1256 ACCGGT A^CCGGT
Alw44I 1249 GTGCAC G^TGCAC
AlwNI 1378 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 167 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1418 AGCGCT AGC^GCT
ApaLI 1249 GTGCAC G^TGCAC
ApoI 963 GAATTT [GA]^AATT[CT]
Asp700I 960 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 142 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1516 CCCGGG C^[CT]CG[GA]G
BalI 308 TGGCCA TGG^CCA
BbeI 1340 GGCGCC GGCGC^C
BbrPI 628 CACGTG CAC^GTG
BsaAI 628 CACGTG [CT]AC^GT[GA]
BsaWI 1256 ACCGGT [AT]^CCGG[AT]
BsmI 1060 GAATGC GAATGC(1/-1)
BsoBI 1516 CCCGGG C^[CT]CG[GA]G
BstAPI 570 GCACCGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 167 CCTCAGG CC^T[ACGT]AGG
DraIII 571 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 429 CGGCCG C^GGCCG
EarI 19 CTCTTC CTCTTC(1/4)
EclXI 429 CGGCCG C^GGCCG
Eco47III 1418 AGCGCT AGC^GCT
Eco52I 429 CGGCCG C^GGCCG
Eco81I 167 CCTCAGG CC^T[ACGT]AGG
FseI 430 GGCCGGCC GGCCGG^CC
HinfI 216 GAATC G^A[ACGT]TC
KasI 1340 GGCGCC G^GCGCC
Ksp632I 19 CTCTTC CTCTTC(1/4)
MluNI 308 TGGCCA TGG^CCA
MscI 308 TGGCCA TGG^CCA
NarI 1340 GGCGCC GG^CGCC
NcoI 1011 CCATGG C^CATGG
PflFI 142 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 311 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1256 ACCGGT A^CCGGT
PmaCI 628 CACGTG CAC^GTG
PmlI 628 CACGTG CAC^GTG
SapI 18 GCTCTTC GCTCTTC(1/4)
SfoI 1340 GGCGCC GGC^GCC
SgrAI 1443 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1516 CCCGGG CCC^GGG
SmlI 727 CTCAAG C^T[CT][GA]AG
StuI 518 AGGCCT AGG^CCT
TfiI 216 GAATC G^A[AT]TC
Tsp509I 964 AATT ^AATT
Tth111I 142 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 311 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 251 CCAACCCGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1516 CCCGGG C^CCGGG
XmaI 1516 CCCGGG C^CCGGG
XmnI 960 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 244 GACGCC G[GA]^CG[CT]C
1340 GGCGCC
AviII 680 TGCGCA TGC^GCA
755 TGCGCA
BsaHI 244 GACGCC G[GA]^CG[CT]C
1340 GGCGCC
BsaI 185 GAGACC GGTCTC(1/5)
1267 GAGACC
BsmAI 185 GAGAC GTCTC(1/5)
1267 GAGAC
BspLU11I 178 ACATGT A^CATGT
551 ACATGT
BssHII 566 GCGCGC G^CGCGC
1511 GCGCGC
BstEII 75 GGTGACC G^GT[ACGT]ACC
1110 GGTGACC
BstF5I 557 CATCC GGATG(2/0)
1200 GGATG
BstPI 75 GGTGACC G^GT[ACGT]ACC
1110 GGTGACC
BstXI 347 CCAGCCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
437 CCACGGTCATGG
DdeI 168 CTCAG C^T[ACGT]AG
194 CTGAG
EcoO65I 75 GGTGACC G^GT[ACGT]ACC
1110 GGTGACC
FokI 557 CATCC GGATG(9/13)
1200 GGATG
FspI 680 TGCGCA TGC^GCA
755 TGCGCA
HgaI 244 GACGC GACGC(5/10)
1118 GCGTC
Hin1I 244 GACGCC G[GA]^CG[CT]C
1340 GGCGCC
PshAI 1113 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1489 GACGGCGGTC
PvuII 1122 CAGCTG CAG^CTG
1378 CAGCTG
ScaI 593 AGTACT AGT^ACT
818 AGTACT
SphI 758 GCATGC GCATG^C
873 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
ApaI AspHI AvaII BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
NspI PstI RsaI Sau96I ScrFI
SfaNI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I