Restriction map for
pF1KSDA1859
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BclI
BfrI BglII BlnI BsiWI BsmI
Bsp1407I BspDI BspLU11I BsrBI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI Eam1105I
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI MfeI MluI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Aor51HI 1923 AGCGCT AGC^GCT
AspHI 1963 GAGCTC G[AT]GC[AT]^C
AspI 67 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2191 GGCGCC GGCGC^C
BbrPI 2082 CACGTG CAC^GTG
BcgI 1872 CGAGGAACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 435 GGATAC GTATCC(6/5)
BpmI 1113 CTGGAG CTGGAG(16/14)
BsiEI 299 CGGCCG CG[GA][CT]^CG
BsiHKAI 1963 GAGCTC G[AT]GC[AT]^C
BsmBI 295 GAGACG CGTCTC(1/5)
BspHI 1538 TCATGA T^CATGA
BsrDI 263 CATTGC GCAATG(2/0)
BssHII 1305 GCGCGC G^CGCGC
BssSI 951 CACGAG CACGAG(-5/-1)
DraIII 403 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1097 CCGCGG C^C[GA][CT]GG
EagI 299 CGGCCG C^GGCCG
EarI 82 GAAGAG CTCTTC(1/4)
EclXI 299 CGGCCG C^GGCCG
Eco47III 1923 AGCGCT AGC^GCT
Eco52I 299 CGGCCG C^GGCCG
KasI 2191 GGCGCC G^GCGCC
Ksp632I 82 GAAGAG CTCTTC(1/4)
KspI 1097 CCGCGG CCGC^GG
MseI 1277 TTAA T^TAA
NaeI 720 GCCGGC GCC^GGC
NarI 2191 GGCGCC GG^CGCC
NgoMIV 720 GCCGGC G^CCGGC
NheI 397 GCTAGC G^CTAGC
PflFI 67 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2082 CACGTG CAC^GTG
PmlI 2082 CACGTG CAC^GTG
PstI 1237 CTGCAG CTGCA^G
RcaI 1538 TCATGA T^CATGA
SacI 1963 GAGCTC GAGCT^C
SacII 1097 CCGCGG CCGC^GG
SfiI 939 GGCCAAGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2191 GGCGCC GGC^GCC
SmaI 1802 CCCGGG CCC^GGG
SmlI 1194 CTCAAG C^T[CT][GA]AG
StuI 192 AGGCCT AGG^CCT
Tru9I 1277 TTAA T^TAA
Tth111I 67 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1802 CCCGGG C^CCGGG
XmaI 1802 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccIII 276 TCCGGA T^CCGGA
2165 TCCGGA
AcsI 112 GAATTC [GA]^AATT[CT]
1717 GAATTC
ApoI 112 GAATTC [GA]^AATT[CT]
1717 GAATTC
BbsI 46 GAAGAC GAAGAC(2/6)
703 GTCTTC
BbvCI 1463 CCTCAGC CCTCAGC(-5/-2)
4 GCTGAGG
BlpI 991 GCTCAGC GC^T[ACGT]AGC
1003 GCTGAGC
Bpu1102I 991 GCTCAGC GC^T[ACGT]AGC
1003 GCTGAGC
BpuAI 46 GAAGAC GAAGAC(2/6)
703 GTCTTC
BsaAI 1201 CACGTA [CT]AC^GT[GA]
2082 CACGTG
BsaBI 1293 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1905 GATGAATATC
BsaWI 276 TCCGGA [AT]^CCGG[AT]
2165 TCCGGA
BseAI 276 TCCGGA T^CCGGA
2165 TCCGGA
BsgI 1449 CTGCAC GTGCAG(16/14)
974 GTGCAG
BspEI 276 TCCGGA T^CCGGA
2165 TCCGGA
BsrFI 668 ACCGGC [GA]^CCGG[CT]
720 GCCGGC
CelII 991 GCTCAGC GC^T[ACGT]AGC
1003 GCTGAGC
Cfr10I 668 ACCGGC [GA]^CCGG[CT]
720 GCCGGC
EcoRI 112 GAATTC G^AATTC
1717 GAATTC
HindIII 12 AAGCTT A^AGCTT
180 AAGCTT
MaeIII 523 GTCAC ^GT[ACGT]AC
1228 GTGAC
MamI 1293 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1905 GATGAATATC
MroI 276 TCCGGA T^CCGGA
2165 TCCGGA
MslI 1051 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1533 CATCTTCATG
NspI 1313 ACATGC [GA]CATG^[CT]
1878 ACATGC
PflMI 246 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
402 CCACCGGGTGG
SfcI 36 CTACAG C^T[GA][CT]AG
1237 CTGCAG
Tsp45I 523 GTCAC ^GT[GC]AC
1228 GTGAC
Tsp509I 113 AATT ^AATT
1718 AATT
Van91I 246 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
402 CCACCGGGTGG
XcmI 819 CCAGACGGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
935 CCAAGGCCAAGATGG
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# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaI AvaII
BalI BamHI BanI BanII BbvI
BcnI BfaI BglI BmyI BsaHI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DrdI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TfiI TseI TspRI TthHB8I
XhoII