Restriction map for
pF1KSDA1862
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII Acc65I AccI AccII AclI
AflII AflIII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbrPI BclI BfrI
BglII BlnI BsaAI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MseI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
ScaI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Tru9I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AcsI 599 GAATTT [GA]^AATT[CT]
AcyI 2519 GGCGCC G[GA]^CG[CT]C
AhdI 1249 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 2457 AGCGCT AGC^GCT
ApoI 599 GAATTT [GA]^AATT[CT]
AspEI 1249 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 436 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 2519 GGCGCC GGCGC^C
BciVI 934 GGATAC GTATCC(6/5)
BsaHI 2519 GGCGCC G[GA]^CG[CT]C
BsmI 1178 GCATTC GAATGC(1/-1)
BstEII 2692 GGTGACC G^GT[ACGT]ACC
BstPI 2692 GGTGACC G^GT[ACGT]ACC
DraIII 1289 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1249 GACTCTAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2457 AGCGCT AGC^GCT
EcoO65I 2692 GGTGACC G^GT[ACGT]ACC
Hin1I 2519 GGCGCC G[GA]^CG[CT]C
KasI 2519 GGCGCC G^GCGCC
MslI 109 CACGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2519 GGCGCC GG^CGCC
PaeR7I 1935 CTCGAG C^TCGAG
PflFI 436 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 18 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 2910 CCTGCAGG CCTGCA^GG
SfiI 1547 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2519 GGCGCC GGC^GCC
Sse8387I 2910 CCTGCAGG CCTGCA^GG
TaqI 1936 TCGA T^CGA
Tth111I 436 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1936 TCGA T^CGA
XhoI 1935 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccIII 2313 TCCGGA T^CCGGA
2328 TCCGGA
AfaI 2124 GTAC GT^AC
2147 GTAC
BamHI 2157 GGATCC G^GATCC
2981 GGATCC
BfaI 710 CTAG C^TAG
1253 CTAG
BlpI 40 GCTGAGC GC^T[ACGT]AGC
348 GCTCAGC
Bpu1102I 40 GCTGAGC GC^T[ACGT]AGC
348 GCTCAGC
BsaI 24 GGTCTC GGTCTC(1/5)
1256 GGTCTC
BsaWI 2313 TCCGGA [AT]^CCGG[AT]
2328 TCCGGA
BseAI 2313 TCCGGA T^CCGGA
2328 TCCGGA
BsmBI 1657 CGTCTC CGTCTC(1/5)
16 GAGACG
BspEI 2313 TCCGGA T^CCGGA
2328 TCCGGA
BssSI 2640 CACGAG CACGAG(-5/-1)
411 CTCGTG
CelII 40 GCTGAGC GC^T[ACGT]AGC
348 GCTCAGC
DsaI 481 CCGTGG C^C[GA][CT]GG
2572 CCACGG
EcoT14I 2526 CCAAGG C^C[AT][AT]GG
2996 CCAAGG
HindIII 690 AAGCTT A^AGCTT
2549 AAGCTT
MaeI 710 CTAG C^TAG
1253 CTAG
MroI 2313 TCCGGA T^CCGGA
2328 TCCGGA
PflMI 1601 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2050 CCATGTCCTGG
RsaI 2124 GTAC GT^AC
2147 GTAC
SacI 832 GAGCTC GAGCT^C
1981 GAGCTC
StyI 2526 CCAAGG C^C[AT][AT]GG
2996 CCAAGG
TfiI 897 GAATC G^A[AT]TC
2505 GAATC
Tsp509I 600 AATT ^AATT
1513 AATT
Van91I 1601 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2050 CCATGTCCTGG
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
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AciI AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcgI BcnI BglI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EarI Eco81I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
PstI PvuII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
TseI Tsp45I TspRI XcmI XhoII